KEGG   ENZYME: 1.1.1.382Help
Entry
EC 1.1.1.382                Enzyme                                 

Name
ketol-acid reductoisomerase (NAD+)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(2R)-2,3-dihydroxy-3-methylbutanoate:NAD+ oxidoreductase (isomerizing)
Reaction(IUBMB)
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+ [RN:R10916]
Reaction(KEGG)
Substrate
(2R)-2,3-dihydroxy-3-methylbutanoate [CPD:C04272];
NAD+ [CPD:C00003]
Product
(2S)-2-hydroxy-2-methyl-3-oxobutanoate [CPD:C06010];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
The enzyme, characterized from the bacteria Thermacetogenium phaeum and Desulfococcus oleovorans and from the archaeon Archaeoglobus fulgidus, is specific for NADH [cf. EC 1.1.1.86, ketol-acid reductoisomerase (NADP+) and EC 1.1.1.383, ketol-acid reductoisomerase [NAD(P)+]].
History
EC 1.1.1.382 created 2015
Reference
1  [PMID:25172159]
  Authors
Brinkmann-Chen S, Cahn JK, Arnold FH
  Title
Uncovering rare NADH-preferring ketol-acid reductoisomerases.
  Journal
Metab Eng 26C:17-22 (2014)
DOI:10.1016/j.ymben.2014.08.003
  Sequence
[dol:Dole_2039] [tpz:Tph_c19850] [afu:AF_1985]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.382
IUBMB Enzyme Nomenclature: 1.1.1.382
ExPASy - ENZYME nomenclature database: 1.1.1.382
BRENDA, the Enzyme Database: 1.1.1.382

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