KEGG   ENZYME: 1.1.1.391Help
Entry
EC 1.1.1.391                Enzyme                                 

Name
3beta-hydroxycholanate 3-dehydrogenase (NAD+);
3beta-hydroxysteroid dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
isolithocholate:NAD+ 3-oxidoreductase
Reaction(IUBMB)
isolithocholate + NAD+ = 3-oxo-5beta-cholan-24-oate + NADH + H+ [RN:R11144]
Reaction(KEGG)
Substrate
isolithocholate [CPD:C17658];
NAD+ [CPD:C00003]
Product
3-oxo-5beta-cholan-24-oate [CPD:C03070];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
This bacterial enzyme is involved, along with EC 1.1.1.52, 3alpha-hydroxycholanate dehydrogenase (NAD+), or EC 1.1.1.392, 3alpha-hydroxycholanate dehydrogenase (NADP+), in the modification of secondary bile acids to form 3beta-bile acids (also known as iso-bile acids). The enzyme catalyses the reaction in the reduction direction in vivo. Also acts on related 3-oxo bile acids. cf. EC 1.1.1.393, 3beta-hydroxycholanate 3-dehydrogenase (NADP+).
History
EC 1.1.1.391 created 2016
Pathway
ec00121  Secondary bile acid biosynthesis
Orthology
K22606  3beta-hydroxycholanate 3-dehydrogenase (NAD+)
Genes
ELE: Elen_0198 Elen_1325
GPA: GPA_22420
Taxonomy
Reference
1  [PMID:2752021]
  Authors
Edenharder R, Pfutzner A, Hammann R
  Title
Characterization of NAD-dependent 3 alpha- and 3 beta-hydroxysteroid dehydrogenase and of NADP-dependent 7 beta-hydroxysteroid dehydrogenase from Peptostreptococcus productus.
  Journal
Biochim Biophys Acta 1004:230-8 (1989)
DOI:10.1016/0005-2760(89)90272-5
Reference
2  [PMID:2764572]
  Authors
Edenharder R, Pfutzner M
  Title
Partial purification and characterization of an NAD-dependent 3 beta-hydroxysteroid dehydrogenase from Clostridium innocuum.
  Journal
Appl Environ Microbiol 55:1656-9 (1989)
Reference
3  [PMID:26192599]
  Authors
Devlin AS, Fischbach MA
  Title
A biosynthetic pathway for a prominent class of microbiota-derived bile acids.
  Journal
Nat Chem Biol 11:685-90 (2015)
DOI:10.1038/nchembio.1864
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.391
IUBMB Enzyme Nomenclature: 1.1.1.391
ExPASy - ENZYME nomenclature database: 1.1.1.391
BRENDA, the Enzyme Database: 1.1.1.391

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