Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy

isolithocholate:NADP+ 3-oxidoreductase

isolithocholate + NADP+ = 3-oxo-5beta-cholan-24-oate + NADPH + H+ [RN:R11146]

R11146
Reaction

isolithocholate [CPD:C17658];
NADP+ [CPD:C00006]

This bacterial enzyme is involved, along with EC 1.1.1.52, 3alpha-hydroxycholanate dehydrogenase (NAD+), or EC 1.1.1.392, 3alpha-hydroxycholanate dehydrogenase (NADP+), in the modification of secondary bile acids to form 3beta-bile acids (also known as iso-bile acids). The enzyme catalyses the reaction in the reduction direction in vivo. Also acts on related 3-oxo bile acids. cf. EC 1.1.1.391, 3beta-hydroxycholanate 3-dehydrogenase (NAD+).

EC 1.1.1.393 created 2016

1  [PMID:3458705]

Akao T, Akao T, Hattori M, Namba T, Kobashi K

3 beta-Hydroxysteroid dehydrogenase of Ruminococcus sp. from human intestinal bacteria.

J Biochem 99:1425-31 (1986)
DOI:10.1093/oxfordjournals.jbchem.a135612

2  [PMID:26192599]

Devlin AS, Fischbach MA

A biosynthetic pathway for a prominent class of microbiota-derived bile acids.

Nat Chem Biol 11:685-90 (2015)
DOI:10.1038/nchembio.1864

[ag:EDN78833]