KEGG   ENZYME: 1.1.1.393Help
Entry
EC 1.1.1.393                Enzyme                                 

Name
3beta-hydroxycholanate 3-dehydrogenase (NADP+);
3beta-hydroxysteroid dehydrogenase (ambiguous)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
isolithocholate:NADP+ 3-oxidoreductase
Reaction(IUBMB)
isolithocholate + NADP+ = 3-oxo-5beta-cholan-24-oate + NADPH + H+ [RN:R11146]
Reaction(KEGG)
Substrate
isolithocholate [CPD:C17658];
NADP+ [CPD:C00006]
Product
3-oxo-5beta-cholan-24-oate [CPD:C03070];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
This bacterial enzyme is involved, along with EC 1.1.1.52, 3alpha-hydroxycholanate dehydrogenase (NAD+), or EC 1.1.1.392, 3alpha-hydroxycholanate dehydrogenase (NADP+), in the modification of secondary bile acids to form 3beta-bile acids (also known as iso-bile acids). The enzyme catalyses the reaction in the reduction direction in vivo. Also acts on related 3-oxo bile acids. cf. EC 1.1.1.391, 3beta-hydroxycholanate 3-dehydrogenase (NAD+).
History
EC 1.1.1.393 created 2016
Pathway
ec00121  Secondary bile acid biosynthesis
Orthology
K22607  3beta-hydroxycholanate 3-dehydrogenase (NADP+)
Reference
1  [PMID:3458705]
  Authors
Akao T, Akao T, Hattori M, Namba T, Kobashi K
  Title
3 beta-Hydroxysteroid dehydrogenase of Ruminococcus sp. from human intestinal bacteria.
  Journal
J Biochem 99:1425-31 (1986)
Reference
2  [PMID:26192599]
  Authors
Devlin AS, Fischbach MA
  Title
A biosynthetic pathway for a prominent class of microbiota-derived bile acids.
  Journal
Nat Chem Biol 11:685-90 (2015)
DOI:10.1038/nchembio.1864
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.393
IUBMB Enzyme Nomenclature: 1.1.1.393
ExPASy - ENZYME nomenclature database: 1.1.1.393
BRENDA, the Enzyme Database: 1.1.1.393

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