KEGG   ENZYME: 1.1.1.398Help
Entry
EC 1.1.1.398                Enzyme                                 

Name
2-glutathionyl-2-methylbut-3-en-1-ol dehydrogenase;
isoH (gene name);
4-hydroxy-3-glutathionyl-3-methylbut-1-ene dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
2-(glutathion-S-yl)-2-methylbut-3-en-1-ol:NAD+ oxidoreductase
Reaction(IUBMB)
2-(glutathion-S-yl)-2-methylbut-3-en-1-ol + 2 NAD+ + H2O = 2-(glutathion-S-yl)-2-methylbut-3-enoate + 2 NADH + 2 H+ (overall reaction);
(1a) 2-(glutathion-S-yl)-2-methylbut-3-en-1-ol + NAD+ = 2-(glutathion-S-yl)-2-methylbut-3-enal + NADH + H+;
(1b) 2-(glutathion-S-yl)-2-methylbut-3-enal + NAD+ + H2O = 2-(glutathion-S-yl)-2-methylbut-3-enoate + NADH + H+
Substrate
2-(glutathion-S-yl)-2-methylbut-3-en-1-ol [CPD:C21374];
NAD+ [CPD:C00003];
H2O [CPD:C00001];
2-(glutathion-S-yl)-2-methylbut-3-enal
Product
2-(glutathion-S-yl)-2-methylbut-3-enoate;
NADH [CPD:C00004];
H+ [CPD:C00080];
2-(glutathion-S-yl)-2-methylbut-3-enal
Comment
The enzyme, characterized from the bacterium Rhodococcus sp. AD45, is involved in isoprene degradation.
History
EC 1.1.1.398 created 2016
Orthology
K21101  2-glutathionyl-2-methylbut-3-en-1-ol dehydrogenase
Genes
ROA: Pd630_LPD03576
Taxonomy
Reference
1  [PMID:10094686]
  Authors
van Hylckama Vlieg JE, Kingma J, Kruizinga W, Janssen DB
  Title
Purification of a glutathione S-transferase and a glutathione conjugate-specific  dehydrogenase involved in isoprene metabolism in Rhodococcus sp. strain AD45.
  Journal
J Bacteriol 181:2094-101 (1999)
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.398
IUBMB Enzyme Nomenclature: 1.1.1.398
ExPASy - ENZYME nomenclature database: 1.1.1.398
BRENDA, the Enzyme Database: 1.1.1.398

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