KEGG   ENZYME: 1.1.1.418Help
Entry
EC 1.1.1.418                Enzyme                                 

Name
plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating);
3beta-HSD/D1 (gene name);
3beta-HSD/D2 (gene name);
3beta-hydroxysteroid dehydrogenases/C-4 decarboxylase (ambiguous)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3beta-hydroxysteroid-4alpha-carboxylate:NAD+ 3-oxidoreductase (decarboxylating)
Reaction(IUBMB)
a 3beta-hydroxysteroid-4alpha-carboxylate + NAD+ = a 3-oxosteroid + CO2 + NADH [RN:R12366]
Reaction(KEGG)
Substrate
3beta-hydroxysteroid-4alpha-carboxylate [CPD:C22111];
NAD+ [CPD:C00003]
Product
3-oxosteroid [CPD:C01876];
CO2 [CPD:C00011];
NADH [CPD:C00004]
Comment
The enzyme, found in plants, catalyses multiple reactions during plant sterol biosynthesis. Unlike the fungal/animal enzyme EC 1.1.1.170, 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating), the plant enzyme is specific for NAD+.
History
EC 1.1.1.418 created 2019
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K23558  plant 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase
Genes
AQU: 105314342
ATH: AT1G47290(3BETAHSD/D1) AT2G26260(3BETAHSD/D2) AT2G43420
ALY: ARALYDRAFT_473882(AT3BETAHSD/D1) ARALYDRAFT_481483(AT3BETAHSD/D2) ARALYDRAFT_483452
CRB: 17888910 17889700 17898596
CSAT: 104703154 104714089 104752257 104758028 104777665 104782858 104785013 104793273 109124732
EUS: EUTSA_v10001381mg EUTSA_v10001949mg EUTSA_v10011495mg
BRP: 103842157 103843901 103866715
BNA: 106368540 106371310 106390851 106400657 106402151 106414952 106421095
BOE: 106296378 106307642 106308495 106341839
RSZ: 108811832 108818777 108856331 108862277 108862423
LJA: Lj0g3v0192789.1(Lj0g3v0192789.1) Lj1g3v1798470.1(Lj1g3v1798470.1) Lj2g3v0856600.1(Lj2g3v0856600.1) Lj2g3v0856630.1(Lj2g3v0856630.1) Lj3g3v3325980.1(Lj3g3v3325980.1) Lj5g3v0855730.1(Lj5g3v0855730.1)
DOSA: Os03t0404800-01(Os03g0404800) Os03t0405000-01(Os03g0405000) Os09t0516500-00(Os09g0516500)
ATS: 109751859(LOC109751859) 109759047(LOC109759047) 109759249(LOC109759249) 109776475(LOC109776475) 109780969(LOC109780969) 109782739(LOC109782739)
PPP: 112282407
DFA: DFA_07193
 » show all
Taxonomy
Reference
1  [PMID:10356290]
  Authors
Rondet S, Taton M, Rahier A
  Title
Identification, characterization, and partial purification of 4 alpha-carboxysterol-C3-dehydrogenase/ C4-decarboxylase from Zea mays.
  Journal
Arch Biochem Biophys 366:249-60 (1999)
Reference
2  [PMID:16835224]
  Authors
Rahier A, Darnet S, Bouvier F, Camara B, Bard M
  Title
Molecular and enzymatic characterizations of novel bifunctional 3beta-hydroxysteroid dehydrogenases/C-4 decarboxylases from Arabidopsis thaliana.
  Journal
J Biol Chem 281:27264-77 (2006)
DOI:10.1074/jbc.M604431200
  Sequence
Reference
3  [PMID:19218365]
  Authors
Rahier A, Bergdoll M, Genot G, Bouvier F, Camara B
  Title
Homology modeling and site-directed mutagenesis reveal catalytic key amino acids  of 3beta-hydroxysteroid-dehydrogenase/C4-decarboxylase from Arabidopsis.
  Journal
Plant Physiol 149:1872-86 (2009)
DOI:10.1104/pp.108.132282
  Sequence
[ath:AT1G47290]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.418
IUBMB Enzyme Nomenclature: 1.1.1.418
ExPASy - ENZYME nomenclature database: 1.1.1.418
BRENDA, the Enzyme Database: 1.1.1.418
CAS: 71822-23-6

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