| Entry |
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| Name |
isocitrate dehydrogenase (NADP+);
oxalosuccinate decarboxylase;
oxalsuccinic decarboxylase;
isocitrate (NADP) dehydrogenase;
isocitrate (nicotinamide adenine dinucleotide phosphate) dehydrogenase;
NADP-specific isocitrate dehydrogenase;
NADP-linked isocitrate dehydrogenase;
NADP-dependent isocitrate dehydrogenase;
NADP isocitric dehydrogenase;
isocitrate dehydrogenase (NADP-dependent);
NADP-dependent isocitric dehydrogenase;
triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase;
NADP+-linked isocitrate dehydrogenase;
IDH (ambiguous);
dual-cofactor-specific isocitrate dehydrogenase;
NADP+-ICDH;
NADP+-IDH;
IDP;
IDP1;
IDP2;
IDP3
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| Class |
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
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| Sysname |
isocitrate:NADP+ oxidoreductase (decarboxylating)
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| Reaction(IUBMB) |
isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+ (overall reaction) [RN: R00267];
(1a) isocitrate + NADP+ = oxalosuccinate + NADPH + H+ [RN: R01899];
(1b) oxalosuccinate = 2-oxoglutarate + CO2 [RN: R00268]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Requires Mn2+ or Mg2+ for activity. Unlike EC 1.1.1.41, isocitrate dehydrogenase (NAD+), oxalosuccinate can be used as a substrate. In eukaryotes, isocitrate dehydrogenase exists in two forms: an NAD+-linked enzyme found only in mitochondria and displaying allosteric properties, and a non-allosteric, NADP+-linked enzyme that is found in both mitochondria and cytoplasm [6]. The enzyme from some species can also use NAD+ but much more slowly [6,7].
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| History |
EC 1.1.1.42 created 1961, modified 2005
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| Pathway |
| ec00720 | Carbon fixation pathways in prokaryotes |
| ec01110 | Biosynthesis of secondary metabolites |
| ec01120 | Microbial metabolism in diverse environments |
| ec01130 | Biosynthesis of antibiotics |
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| Orthology |
| K00031 | isocitrate dehydrogenase |
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| Genes |
» show all
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| Reference |
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| Authors |
AGOSIN M, WEINBACH EC. |
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| Title |
Partial purification and characterization of the isocitric dehydrogenase from Trypanosoma cruzi. |
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| Journal |
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| Reference |
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| Authors |
DIXON M, MOYLE J. |
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| Title |
Purification of the isocitric enzyme (triphosphopyridine nucleotide-linked isocitric dehydrogenase-oxalosuccinic carboxylase). |
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| Journal |
Biochem J 63:548-52 (1956) |
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| Reference |
3 |
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| Authors |
Plaut, G.W.E. |
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| Title |
Isocitrate dehydrogenases. |
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| Journal |
In: Boyer, P.D., Lardy, H. and Myrback, K. (Eds.), The Enzymes, 2nd ed., vol. 7, Academic Press, New York, 1963, p. 105-126. |
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| Reference |
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| Authors |
SIEBERT G, DUBUC J, WARNER RC, PLAUT GW. |
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| Title |
The preparation of isocitric dehydrogenase from mammalian heart. |
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| Journal |
J Biol Chem 226:965-75 (1957) |
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| Reference |
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| Authors |
VICKERY HB. |
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| Title |
A suggested new nomenclature for the isomers of isocitric acid. |
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| Journal |
J Biol Chem 237:1739-41 (1962) |
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| Reference |
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| Authors |
Camacho ML, Brown RA, Bonete MJ, Danson MJ, Hough DW. |
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| Title |
Isocitrate dehydrogenases from Haloferax volcanii and Sulfolobus solfataricus: enzyme purification, characterisation and N-terminal sequence. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Steen IH, Lien T, Birkeland NK. |
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| Title |
Biochemical and phylogenetic characterization of isocitrate dehydrogenase from a hyperthermophilic archaeon, Archaeoglobus fulgidus. |
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| Journal |
Arch Microbiol 168:412-20 (1997) |
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| Sequence |
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| Reference |
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| Authors |
Koh HJ, Lee SM, Son BG, Lee SH, Ryoo ZY, Chang KT, Park JW, Park DC, Song BJ, Veech RL, Song H, Huh TL. |
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| Title |
Cytosolic NADP+-dependent isocitrate dehydrogenase plays a key role in lipid metabolism. |
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| Journal |
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| Reference |
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| Authors |
Ceccarelli C, Grodsky NB, Ariyaratne N, Colman RF, Bahnson BJ. |
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| Title |
Crystal structure of porcine mitochondrial NADP+-dependent isocitrate dehydrogenase complexed with Mn2+ and isocitrate. Insights into the enzyme mechanism. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 1.1.1.42 |
| ExPASy - ENZYME nomenclature database: | 1.1.1.42 |
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