KEGG   ENZYME: 1.1.2.10
Entry
EC 1.1.2.10                 Enzyme                                 

Name
lanthanide-dependent methanol dehydrogenase;
XoxF;
XoxF-MDH;
Ce-MDH;
La3+-dependent MDH;
Ce3+-induced methanol dehydrogenase;
cerium dependent MDH
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With a cytochrome as acceptor
Sysname
methanol:cytochrome cL oxidoreductase
Reaction(IUBMB)
methanol + 2 oxidized cytochrome cL = formaldehyde + 2 reduced cytochrome cL [RN:R01146]
Reaction(KEGG)
R01146
Substrate
methanol [CPD:C00132];
oxidized cytochrome cL [CPD:C18233]
Product
formaldehyde [CPD:C00067];
reduced cytochrome cL [CPD:C18234]
Comment
Isolated from the bacterium Methylacidiphilum fumariolicum and many Methylobacterium species. Requires La3+, Ce3+, Pr3+ or Nd3+. The higher lanthanides show decreasing activity with Sm3+, Eu3+ and Gd3+. The lanthanide is coordinated by the enzyme and pyrroloquinoline quinone. Shows little activity with Ca2+, the required cofactor of EC 1.1.2.7, methanol dehydrogenase (cytochrome c).
History
EC 1.1.2.10 created 2019
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K23995  lanthanide-dependent methanol dehydrogenase
Genes
XCC: XCC3482(mxaF)
XCB: XC_0679
XCA: xcc-b100_0716(mxaF1)
XCP: XCR_3815
XCV: XCV0708(mxaF)
XAX: XACM_0650(mxaF)
XAC: XAC0648(mxaF)
XCI: XCAW_03933(mxaF)
XCT: J151_00769
XCJ: J158_00766
XPH: XppCFBP6546_08380(XppCFBP6546P_08380)
DKO: I596_1053
MCA: MCA0299
METL: U737_19560
MAH: MEALZ_3497(xoxF)
NHL: Nhal_3866
GAI: IMCC3135_00470(moxF)
BXE: Bxe_B2427
BXB: DR64_4756
BPH: Bphy_6478
AMIM: MIM_c14460
VEI: Veis_4972
MPT: Mpe_A3393
LCH: Lcho_3106
RGE: RGE_40660(xoxF)
MBAC: BN1209_1575(xoxF)
MBAT: BN1208_1208(xoxF)
SME: SM_b20173
SMI: BN406_06484(xoxF)
SMER: DU99_32470
SFD: USDA257_c30950(xoxF1) USDA257_c54310(xoxF2)
RHT: NT26_3262(mxaF)
BJA: blr6213(mxaF')
BRA: BRADO5484(mxaF')
BBT: BBta_5968(mxaF')
BRS: S23_20820(mxaF')
AOL: S58_22240
BRO: BRAD285_1749(mxaF) BRAD285_2933(xoxF)
RPE: RPE_0851
SNO: Snov_1035
MEA: Mex_1p1740(xoxF) Mex_1p2757(xoxF)
MDI: METDI2492
MPO: Mpop_1761
MAQU: Maq22A_c05215(xoxF1) Maq22A_c27990(xoxF2)
BBAR: RHAL1_02998(xoxF)
FIL: BN1229_v1_0562(xoxF) BN1229_v1_2382(xoxF)
FIY: BN1229_v1_0564(xoxF) BN1229_v1_3533(xoxF)
MSC: BN69_1363
PLEO: OHA_1_00064(xoxF1) OHA_1_01165(moxF_1) OHA_1_03539(moxF_2) OHA_1_04184(moxF_4)
HDI: HDIA_2042(moxF) HDIA_2631
RSP: RSP_2578(xoxF)
RDE: RD1_0890(moxF)
PDE: Pden_0020
DSH: Dshi_0476(gcd)
RHC: RGUI_0956
RMM: ROSMUCSMR3_01466(moxF)
ROH: FIU89_13740(moxF)
MALU: KU6B_56410
ACR: Acry_2790
ALI: AZOLI_p10703(moxF2)
ABS: AZOBR_50044(mxaF) AZOBR_p270106(moxF)
LPIL: LIP_1128
MIN: Minf_0992(gcd)
MKC: kam1_1014(gcd)
SUS: Acid_6912
HTH: HTH_0392
MOX: DAMO_0124(mxaF) DAMO_0134(mxaF)
 » show all
Reference
1  [PMID:21256798]
  Authors
Hibi Y, Asai K, Arafuka H, Hamajima M, Iwama T, Kawai K
  Title
Molecular structure of La3+-induced methanol dehydrogenase-like protein in Methylobacterium radiotolerans.
  Journal
J Biosci Bioeng 111:547-9 (2011)
DOI:10.1016/j.jbiosc.2010.12.017
  Sequence
Reference
2  [PMID:23209751]
  Authors
Nakagawa T, Mitsui R, Tani A, Sasa K, Tashiro S, Iwama T, Hayakawa T, Kawai K
  Title
A catalytic role of XoxF1 as La3+-dependent methanol dehydrogenase in Methylobacterium extorquens strain AM1.
  Journal
PLoS One 7:e50480 (2012)
DOI:10.1371/journal.pone.0050480
Reference
3  [PMID:24034209]
  Authors
Pol A, Barends TR, Dietl A, Khadem AF, Eygensteyn J, Jetten MS, Op den Camp HJ
  Title
Rare earth metals are essential for methanotrophic life in volcanic mudpots.
  Journal
Environ Microbiol 16:255-64 (2014)
DOI:10.1111/1462-2920.12249
Reference
4  [PMID:25421364]
  Authors
Bogart JA, Lewis AJ, Schelter EJ
  Title
DFT study of the active site of the XoxF-type natural, cerium-dependent methanol  dehydrogenase enzyme.
  Journal
Chemistry 21:1743-8 (2015)
DOI:10.1002/chem.201405159
Reference
5  [PMID:28488399]
  Authors
Prejano M, Marino T, Russo N
  Title
How Can Methanol Dehydrogenase from Methylacidiphilum fumariolicum Work with the  Alien Ce(III) Ion in the Active Center? A Theoretical Study.
  Journal
Chemistry 23:8652-8657 (2017)
DOI:10.1002/chem.201700381
Reference
6  [PMID:29404411]
  Authors
Masuda S, Suzuki Y, Fujitani Y, Mitsui R, Nakagawa T, Shintani M, Tani A
  Title
Lanthanide-Dependent Regulation of Methylotrophy in Methylobacteriumaquaticum Strain 22A.
  Journal
mSphere 3 (2018)
DOI:10.1128/mSphere.00462-17
Other DBs
ExplorEnz - The Enzyme Database: 1.1.2.10
IUBMB Enzyme Nomenclature: 1.1.2.10
ExPASy - ENZYME nomenclature database: 1.1.2.10
BRENDA, the Enzyme Database: 1.1.2.10

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