KEGG   ENZYME: 1.1.2.10
Entry
EC 1.1.2.10                 Enzyme                                 
Name
lanthanide-dependent methanol dehydrogenase;
XoxF;
XoxF-MDH;
Ce-MDH;
La3+-dependent MDH;
Ce3+-induced methanol dehydrogenase;
cerium dependent MDH
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With a cytochrome as acceptor
Sysname
methanol:cytochrome cL oxidoreductase
Reaction(IUBMB)
methanol + 2 oxidized cytochrome cL = formaldehyde + 2 reduced cytochrome cL [RN:R01146]
Reaction(KEGG)
R01146
Substrate
methanol [CPD:C00132];
oxidized cytochrome cL [CPD:C18233]
Product
formaldehyde [CPD:C00067];
reduced cytochrome cL [CPD:C18234]
Comment
Isolated from the bacterium Methylacidiphilum fumariolicum and many Methylobacterium species. Requires La3+, Ce3+, Pr3+ or Nd3+. The higher lanthanides show decreasing activity with Sm3+, Eu3+ and Gd3+. The lanthanide is coordinated by the enzyme and pyrroloquinoline quinone. Shows little activity with Ca2+, the required cofactor of EC 1.1.2.7, methanol dehydrogenase (cytochrome c).
History
EC 1.1.2.10 created 2019
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K23995  lanthanide-dependent methanol dehydrogenase
Genes
XCCXCC3482(mxaF)
XCBXC_0679
XCAxcc-b100_0716(mxaF1)
XCPXCR_3815
XCVXCV0708(mxaF)
XAXXACM_0650(mxaF)
XACXAC0648(mxaF)
XCIXCAW_03933(mxaF)
XCTJ151_00769
XCJJ158_00766
XCUJ159_00766
XCNJ169_00765
XCWJ162_00765
XCRJ163_00765
XCMJ164_00765
XCFJ172_00760
XFUXFF4834R_chr06570
XAOXAC29_03290
XSASB85_16585
XVEBJD12_16090
XPEBJD13_13465
XHRXJ27_19360
XGABI317_20235
XPHXppCFBP6546_08380(XppCFBP6546P_08380)
XANAC801_03225
XARXB05_20540
XHYFZ025_12040
XEUXSP_000670
XHDLMG31886_07640
XPRMUG10_06930
XAGHEP73_00749
XDYNYR95_03850
XASHEP74_03387
LSOLGOY17_09755
LAUXLA521A_16270
FEDLQ772_00030
DTXATSB10_32660
DKOI596_1053
GHOAL542_11475
GKDK6Q96_15225
HMIsoil367_13540
MCAMCA0299
METUGNH96_01870
MMEOOOT43_14340
MMTMetme_1518
MDNJT25_014335
MDHAYM39_03800
MKOMKLM6_0794
METLU737_19560
MPADKEF85_10445 KEF85_13950
MELLIVG45_02000 IVG45_12690
MRPNM686_007660
MMOTQZJ86_04010
MAHMEALZ_3497(xoxF)
MBUREQU24_18605
MPSYCEK71_18090 CEK71_20830
MMAIsS8_0935 sS8_1979 sS8_2523
MSZEMSZNOR_0304(xoxF) MSZNOR_2631(xoxF)
MMOBF6R98_11505 F6R98_16780
MEINmethR_P0358
MOZMoryE10_30670
MISZMishRS11D_09070 MishRS11D_10310
MESLKKZ03_00565
MEIYMIN45_P1657
MCAUMIT9_P2026
MEJQ7A_1457 Q7A_1973 Q7A_622 Q7A_864
MECQ7C_1406 Q7C_2209
MPINLGT42_000005 LGT42_004275
MMAFGCM100_12020 GCM100_19500 GCM100_21160 GCM100_21170(mxaF') GCM100_21180
BLEPAL038_14950
NHLNhal_3866
GAIIMCC3135_00470(moxF)
HHUAR456_03050
HALOBWR19_16685
HCAMI4484_18720
HSXHNO51_18675
HSVHNO53_18995
HTXEKK97_20930
BUGBC1001_3943
BGFBC1003_5460
BYIBYI23_B014510
BUEBRPE67_BCDS01500
BUQAC233_04735 AC233_22000
BXEBxe_B2427
BXBDR64_4756
BPHBphy_6478
BGEBC1002_3228
BPXBUPH_00526
BCAIK788_0008552
PSPWBJG93_19370
PARABTO02_24150
PARBCJU94_31220
PHSC2L64_38320
PTERC2L65_34315
PGPCUJ91_05220 CUJ91_21525
PCJCUJ87_23395
PTSCUJ90_28095
PGISI6I06_22440
PEWKZJ38_25900
PDIOPDMSB3_0634.1(xoxF)
PSAAQEN71_32845
PKFRW095_18830
BUOBRPE64_BCDS01640
CABKNK8_41280
TCARU0034_17555
AKATKWG_15965
AMIMMIM_c14460
VEIVeis_4972
VAPVapar_0700 Vapar_3040
VPDVAPA_1c31060
MPTMpe_A3393
METPC1M51_06800
RGERGE_40660(xoxF)
ATERMW290_06880
LCHLcho_3106
MFYHH212_09670
MLIRLPB04_01640 LPB04_04570
MALIEYF70_29615
MUMFCL38_20010
MFLAGO485_11185 GO485_25035
MPLIE1742_23870
ACTBRHM62_12595
METRBSY238_2804 BSY238_2805
MFAMfla_0344 Mfla_1451 Mfla_2314
MMBMmol_1770 Mmol_2048
MEHM301_1208 M301_1659 M301_2463
MEIMsip34_0016 Msip34_1813 Msip34_2348 Msip34_2549
MEPMPQ_0016(gcd) MPQ_1818 MPQ_2496
MBACBN1209_1575(xoxF)
MBATBN1208_1208(xoxF)
MEUACJ67_06700 ACJ67_12715 ACJ67_14150
MPAUZMTM_15930 ZMTM_22140 ZMTM_25490
AZAAZKH_p0259
THUAC731_004820
THAUC4PIVTH_3693(xoxF)
THKCCZ27_13285
AZQG3580_02750
MLNA9174_19645
MCIMesci_4297 Mesci_6202
MCICA4R28_05575 A4R28_32435
MOPMesop_4366
MAMMesau_04080
MESWA9K65_023805 A9K65_034175
MESMEJ066_29950
MOHIHQ72_13665
MMEILRP31_13810
PHTBLM14_23910
SMESM_b20173
SMKSinme_3993
SMQSinmeB_4490
SMXSM11_pD1430
SMIBN406_06484(xoxF)
SMEGC770_GR4pD1416
SMELSM2011_b20173
SMERDU99_32470
SMDSmed_3943 Smed_6351
SFHSFHH103_05904
SFDUSDA257_c30950(xoxF1) USDA257_c54310(xoxF2)
SAMESAMCFNEI73_Ch0092(xoxF) SAMCFNEI73_pC0377(xoxF)
SINOSS05631_c38640
ESJSJ05684_b47680 SJ05684_b58950
EMXFKV68_00760 FKV68_33145
ENUPYH37_001447 PYH37_003874
EGIPZN02_001154 PZN02_004165
SKMPZL22_000951
STEGQA637_17235 QA637_25260
EAHFA04_27875
RGARGR602_PC01545
RSULN2599_31080
NPMQEO92_19100
RHTNT26_3262(mxaF)
ABAWD5400_04045
BJAblr6213(mxaF')
BJUBJ6T_34880
BJPRN69_16920
BRABRADO5484(mxaF')
BBTBBta_5968(mxaF')
BRSS23_20820(mxaF')
AOLS58_22240
BRCBCCGELA001_15250 BCCGELA001_26700
BRADBF49_5268 BF49_5357 BF49_5363 BF49_5368
BICLMTR13_05740 LMTR13_13665
BROBRAD285_1749(mxaF) BRAD285_2933(xoxF)
BRKCWS35_32595
BOTCIT37_23165
BRQCIT40_23240
BGQX265_08495 X265_11190
BGZXH91_10415
BSYMCIT39_22235
BBETF8237_25290
BARHWN72_34680
BVZBRAD3257_2934(xoxF) BRAD3257_6337(xoxF)
BELBE61_38340
BDGLPJ38_32945
BSEPHAP48_0048230
BQBJ4P68_0007440
BBANJ4G43_037130
BCANBcanWSM471_27575 BcanWSM471_28005
BAUTQA635_14755
BBRAQA636_14485
BGKIC762_25695
BCOUIC761_10440
BPAHQA639_30335
BXNI3J27_27095
BROSQUH67_13110
BRAZLRP30_28655
RPERPE_0851
TRBHB776_18795
TALZRPMA_04795
XAUXaut_1781 Xaut_4686
XDIEZH22_07465
STARG3545_05010 G3545_09685
LNEFZC33_09215
SNOSnov_1035
ANCGBB76_01675
APRAG3A50_17630 G3A50_20050
APOLK9D25_19075
MEAMex_1p1740(xoxF) Mex_1p2757(xoxF)
MDIMETDI2492
MEXMext_0099 Mext_1809
MCHMchl_2145 Mchl_5444
MPOMpop_1761
MZAB2G69_16705
MRDMrad2831_0508 Mrad2831_1932
METM446_2082 M446_2994 M446_5752
MNOMnod_0421 Mnod_2344 Mnod_5977
MORMOC_0664 MOC_2340
METAY590_08660 Y590_19095
MAQUMaq22A_c05215(xoxF1) Maq22A_c27990(xoxF2)
MPHYMCBMB27_00085 MCBMB27_01555
MEEDA075_09885 DA075_21965
METDC0214_10415 C0214_21230
METXA3862_21280 A3862_28410
METSDK389_01595
METIDK427_20455
MMESMMSR116_16580 MMSR116_22465
MTEADK419_15175 DK419_17835
MINDmvi_46800 mvi_49900
MOGMMB17_05055 MMB17_11850 MMB17_20135(xoxF)
MTADM6G65_04760 M6G65_13775
MOCBB934_29530
MICOGDR74_15460
MTEZHPT29_023400
MLDU0023_06155 U0023_24430 U0023_26470
MSLMsil_1587 Msil_2260 Msil_3149 Msil_3387
MTUNMTUNDRAET4_0121.1(xoxF3) MTUNDRAET4_1637(xoxF1) MTUNDRAET4_3562(xoxF5) MTUNDRAET4_3771(xoxF5-2)
MLGCWB41_02560 CWB41_08010 CWB41_10035 CWB41_12120
MEDKQEV83_18970
BBARRHAL1_02998(xoxF)
HDNHden_1305 Hden_1617 Hden_1869 Hden_2848
HDTHYPDE_23038 HYPDE_23343 HYPDE_25993 HYPDE_26383 HYPDE_29963 HYPDE_37788
HMCHYPMC_0800 HYPMC_1896 HYPMC_3613 HYPMC_4120
HNIW911_03300 W911_13290 W911_16160
FILBN1229_v1_0562(xoxF) BN1229_v1_2382(xoxF)
FIYBN1229_v1_0564(xoxF) BN1229_v1_3533(xoxF)
MCGGL4_0437 GL4_1130 GL4_1360 GL4_1361
METGHT051_03065 HT051_11780
MSCBN69_1363
MBRYB1812_05555 B1812_07070
MROSEHO51_13105
MHEYH2LOC_003720 H2LOC_008405 H2LOC_017605
MPARF7D14_03490 F7D14_03920 F7D14_05105
MIWASS37A_00170(mxaF') SS37A_33470 SS37A_35570
MTWCQW49_14395 CQW49_15840
PLEOOHA_1_00064(xoxF1) OHA_1_01165(moxF_1) OHA_1_03539(moxF_2) OHA_1_04184(moxF_4)
HDIHDIA_2042(moxF) HDIA_2631
AALAIGS74_05660
AALMLUX29_06415
AUZSa4125_38750
AUBLXB15_00835 LXB15_14965
JAVOXU80_20900
BRND1F64_06495
NOHG5V57_12150
ACUTMRB58_04380
RUAD1823_18595
RDERD1_0890(moxF)
RLIRLO149_c037700
DSHDshi_0476(gcd)
YPACCEW88_21790
YANAYJ57_23725
TPROGa0080559_TMP1770
PABYGa0080574_TMP283
RMMROSMUCSMR3_01466(moxF)
ROKRAK1035_1700
ROHFIU89_13740(moxF)
SAGUCDO87_22695
OCDFHY55_11650
RMHLVO79_17305
MALUKU6B_56410
PALXGQA70_00445
RSPRSP_2578(xoxF)
RSHRsph17029_1236
RSQRsph17025_1945
RSKRSKD131_0890
CAZTLV780_08620
PDEPden_0020
PAMIJCM7686_0090
PYEA6J80_08640
PCONB0A89_05630
PAROCUV01_07535
PAMNJCM7685_0197
PARREOJ32_02175
PAAKFIU66_13715
PKDF8A10_00235
PPANESD82_21230
PLIAE4191_14860
PMAUCP157_00045(moxF)
PMEHJWJ88_10740
PSEWJHW44_01030
PSAPJHX88_20910
PAEXJHW48_00115
PSTLJHW45_00490
PALPJHW40_18900
PVERE3U25_19910
PFEOE3U26_08185
RHCRGUI_0956
DAAAKL17_0668
HMLHmaOT1_17500
PPRUFDP22_18705
PAMOBAR1_01250
PGVSL003B_1016
AMAQGO499_15620
NOGGKE62_05770
SCABLZK98_17585
GBEGbCGDNIH1_0122 GbCGDNIH1_0651 GbCGDNIH1_1922
GBHGbCGDNIH2_0122 GbCGDNIH2_0651 GbCGDNIH2_1922
GBCGbCGDNIH3_0122 GbCGDNIH3_0651 GbCGDNIH3_1922
GBSGbCGDNIH4_0122 GbCGDNIH4_0651 GbCGDNIH4_1922
ACRAcry_2790
AMVACMV_31220
RMTIAI58_19060 IAI58_19585
RAPRHOA_6230(xoxF)
ALIAZOLI_p10703(moxF2)
ABSAZOBR_50044(mxaF) AZOBR_p270106(moxF)
ABQABAZ39_14850 ABAZ39_25350
ABFAMK58_22450
ATIAL072_26995
AZTTSH58p_01085
AZMDM194_17165
AZZDEW08_25260
AARED3093_25080
AZSE6C72_22375
NAOY958_31085
SKTIGS68_06280
SMUCJL100_021340
SROEJL101_020035
LPILLIP_1128
MINMinf_0992(gcd)
MKCkam1_1014(gcd)
MFHMFUM_1183(xoxF)
MCAOIT6_01685
MEAPMTHMO_1700(xoxF1) MTHMO_1756(xoxF2)
ORPMOP44_12205 MOP44_26705
SUSAcid_6912
GBAJ421_0297 J421_1793 J421_4070
HTHHTH_0392
HTEHydth_0390
MOXDAMO_0124(mxaF) DAMO_0134(mxaF)
 » show all
Reference
1  [PMID:21256798]
  Authors
Hibi Y, Asai K, Arafuka H, Hamajima M, Iwama T, Kawai K
  Title
Molecular structure of La3+-induced methanol dehydrogenase-like protein in Methylobacterium radiotolerans.
  Journal
J Biosci Bioeng 111:547-9 (2011)
DOI:10.1016/j.jbiosc.2010.12.017
  Sequence
Reference
2  [PMID:23209751]
  Authors
Nakagawa T, Mitsui R, Tani A, Sasa K, Tashiro S, Iwama T, Hayakawa T, Kawai K
  Title
A catalytic role of XoxF1 as La3+-dependent methanol dehydrogenase in Methylobacterium extorquens strain AM1.
  Journal
PLoS One 7:e50480 (2012)
DOI:10.1371/journal.pone.0050480
Reference
3  [PMID:24034209]
  Authors
Pol A, Barends TR, Dietl A, Khadem AF, Eygensteyn J, Jetten MS, Op den Camp HJ
  Title
Rare earth metals are essential for methanotrophic life in volcanic mudpots.
  Journal
Environ Microbiol 16:255-64 (2014)
DOI:10.1111/1462-2920.12249
Reference
4  [PMID:25421364]
  Authors
Bogart JA, Lewis AJ, Schelter EJ
  Title
DFT study of the active site of the XoxF-type natural, cerium-dependent methanol  dehydrogenase enzyme.
  Journal
Chemistry 21:1743-8 (2015)
DOI:10.1002/chem.201405159
Reference
5  [PMID:28488399]
  Authors
Prejano M, Marino T, Russo N
  Title
How Can Methanol Dehydrogenase from Methylacidiphilum fumariolicum Work with the  Alien Ce(III) Ion in the Active Center? A Theoretical Study.
  Journal
Chemistry 23:8652-8657 (2017)
DOI:10.1002/chem.201700381
Reference
6  [PMID:29404411]
  Authors
Masuda S, Suzuki Y, Fujitani Y, Mitsui R, Nakagawa T, Shintani M, Tani A.
  Title
Lanthanide-Dependent Regulation of Methylotrophy in Methylobacteriumaquaticum Strain 22A.
  Journal
mSphere 3:e00462-17 (2018)
DOI:10.1128/mSphere.00462-17
Other DBs
ExplorEnz - The Enzyme Database: 1.1.2.10
IUBMB Enzyme Nomenclature: 1.1.2.10
ExPASy - ENZYME nomenclature database: 1.1.2.10
BRENDA, the Enzyme Database: 1.1.2.10

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