KEGG ENZYME: 1.1.2.10

ENZYME: 1.1.2.10

Entry

EC 1.1.2.10                 Enzyme

Name

lanthanide-dependent methanol dehydrogenase;
XoxF;
XoxF-MDH;
Ce-MDH;
La3+-dependent MDH;
Ce3+-induced methanol dehydrogenase;
cerium dependent MDH

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With a cytochrome as acceptor

Sysname

methanol:cytochrome cL oxidoreductase

Reaction(IUBMB)

methanol + 2 oxidized cytochrome cL = formaldehyde + 2 reduced cytochrome cL [RN:R01146]

Reaction(KEGG)

R01146

Substrate

methanol [CPD:C00132];
oxidized cytochrome cL [CPD:C18233]

Product

formaldehyde [CPD:C00067];
reduced cytochrome cL [CPD:C18234]

Comment

Isolated from the bacterium Methylacidiphilum fumariolicum and many Methylobacterium species. Requires La3+, Ce3+, Pr3+ or Nd3+. The higher lanthanides show decreasing activity with Sm3+, Eu3+ and Gd3+. The lanthanide is coordinated by the enzyme and pyrroloquinoline quinone. Shows little activity with Ca2+, the required cofactor of EC 1.1.2.7, methanol dehydrogenase (cytochrome c).

History

EC 1.1.2.10 created 2019

Pathway

ec00680

Methane metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K23995

lanthanide-dependent methanol dehydrogenase

Genes

XCC:	XCC3482(mxaF)

XCB:

XC_0679

XCA:	xcc-b100_0716(mxaF1)

XCP:

XCR_3815

XCV:	XCV0708(mxaF)

XAX:	XACM_0650(mxaF)

XAC:	XAC0648(mxaF)

XCI:	XCAW_03933(mxaF)

XCT:	J151_00769

XCJ:	J158_00766

XCU:	J159_00766

XCN:	J169_00765

XCW:	J162_00765

XCR:	J163_00765

XCM:	J164_00765

XCF:	J172_00760

XFU:	XFF4834R_chr06570

XAO:	XAC29_03290

XSA:	SB85_16585

XGM:	KFS84_03795

XVE:	BJD12_16090

XPE:	BJD13_13465

XHR:	XJ27_19360

XGA:	BI317_20235

XPH:	XppCFBP6546_08380(XppCFBP6546P_08380)

XAN:	AC801_03225

XAR:	XB05_20540

XHY:	FZ025_12040

XEU:	XSP_000670

XHD:	LMG31886_07640

XPR:	MUG10_06930

XAG:	HEP73_00749

XDY:	NYR95_03850

XAS:	HEP74_03387

XHA:	PG878_16705

XRY:	QN244_03905

XSO:	RAB70_07345

XIN:	Q7W82_19665

XAI:	HEP75_03461

XAZ:	XSP_000671

LSOL:

GOY17_09755

LAUX:

LA521A_16270

FED:	LQ772_00030

DTX:	ATSB10_32660

DYG:	DYGSA30_09630(mxaF')

DKO:

I596_1053

GHO:	AL542_11475

GKD:	K6Q96_15225

HMI:	soil367_13540

MCA:

MCA0299

METU:

GNH96_01870

MMEO:

OOT43_14340

MEEF:

JWZ97_07535 JWZ97_15225

MMT:	Metme_1518

MDN:	JT25_014335

MDH:	AYM39_03800

MKO:	MKLM6_0794

METL:

U737_19560

MPAD:

KEF85_10445 KEF85_13950

MELL:

IVG45_02000 IVG45_12690

MRP:	NM686_007660

MMOT:

QZJ86_04010

MEEP:

JWZ98_17905

MEEC:

PL263_18300

MEUP:

QC632_01130 QC632_20185

MAH:	MEALZ_3497(xoxF)

MBUR:

EQU24_18605

MPSY:

CEK71_18090 CEK71_20830

MMAI:

sS8_0935 sS8_1979 sS8_2523

MSZE:

MSZNOR_0304(xoxF) MSZNOR_2631(xoxF)

MMOB:

F6R98_11505 F6R98_16780

MEIN:

methR_P0358

MOZ:	MoryE10_30670

MISZ:

MishRS11D_09070 MishRS11D_10310

MESL:

KKZ03_00565

MECH:

Q9L42_019085

MEIY:

MIN45_P1657

MCAU:

MIT9_P2026

MEJ:	Q7A_1457 Q7A_1973 Q7A_622 Q7A_864

MEC:	Q7C_1406 Q7C_2209

MPIN:

LGT42_000005 LGT42_004275

MMAF:

GCM100_12020 GCM100_19500 GCM100_21160 GCM100_21170(mxaF') GCM100_21180

MTHA:

VSX76_01900 VSX76_03960 VSX76_14530 VSX76_14540 VSX76_14545 VSX76_14560 VSX76_15175

BLEP:

AL038_14950

NHL:

Nhal_3866

GAI:	IMCC3135_00470(moxF)

HHU:	AR456_03050

HALO:

BWR19_16685

HSV:	HNO53_18995

HCAM:

I4484_18720

HSX:	HNO51_18675

HTX:	EKK97_20930

BUG:	BC1001_3943

BGF:	BC1003_5460

BYI:	BYI23_B014510

BUQ:	AC233_04735 AC233_22000

BURT:

BTHE68_38380(mxaF')

BXE:

Bxe_B2427

BXB:

DR64_4756

BPH:

Bphy_6478

BGE:	BC1002_3228

BPX:	BUPH_00526

BCAI:

K788_0008552

PSPW:

BJG93_19370

PARA:

BTO02_24150

PARB:

CJU94_31220

PHS:	C2L64_38320

PTER:

C2L65_34315

PGP:	CUJ91_05220 CUJ91_21525

PCJ:	CUJ87_23395

PTS:	CUJ90_28095

PGIS:

I6I06_22440

PEW:	KZJ38_25900

PDIO:

PDMSB3_0634.1(xoxF)

PSAA:

QEN71_32845

PKF:	RW095_18830

PHUN:

U0042_02285

PLAD:

PPGU16_69500(mxaF')

PAZZ:

PBP221_72020

BUO:	BRPE64_BCDS01640

CABK:

NK8_41280

BUE:	BRPE67_BCDS01500

TCAR:

U0034_17555

AKA:	TKWG_15965

AMIM:

MIM_c14460

VEI:

Veis_4972

VAP:	Vapar_0700 Vapar_3040

VPD:	VAPA_1c31060

MPT:

Mpe_A3393

METP:

C1M51_06800

RGE:	RGE_40660(xoxF)

SMIO:

CATMQ487_36040

ATER:

MW290_06880

LCH:

Lcho_3106

MFY:	HH212_09670

MLIR:

LPB04_01640 LPB04_04570

MALI:

EYF70_29615

MUM:	FCL38_20010

MFLA:

GO485_11185 GO485_25035

MPLI:

E1742_23870

PLUE:

EWM63_13745

ACTB:

RHM62_12595

METR:

BSY238_2804 BSY238_2805

MFA:	Mfla_0344 Mfla_1451 Mfla_2314

MMB:	Mmol_1770 Mmol_2048

MEH:	M301_1208 M301_1659 M301_2463

MEI:	Msip34_0016 Msip34_1813 Msip34_2348 Msip34_2549

MEP:	MPQ_0016(gcd) MPQ_1818 MPQ_2496

MBAC:

BN1209_1575(xoxF)

MBAT:

BN1208_1208(xoxF)

MRK:	FIT61_01165

MUV:	FIT94_05620

MEU:	ACJ67_06700 ACJ67_12715 ACJ67_14150

MMEC:

FIU01_05295 FIU01_10875 FIU01_12085

MEDZ:

MTDW_03340 MTDW_16260 MTDW_27570

MPAU:

ZMTM_15930 ZMTM_22140 ZMTM_25490

AZA:	AZKH_p0259

THU:	AC731_004820

THAU:

C4PIVTH_3693(xoxF)

THK:	CCZ27_13285

THAG:

CKCBHOJB_02586

AZQ:	G3580_02750

MLN:	A9174_19645

MCI:	Mesci_4297 Mesci_6202

MCIC:

A4R28_05575 A4R28_32435

MOP:	Mesop_4366

MAM:	Mesau_04080

MESW:

A9K65_023805 A9K65_034175

MESM:

EJ066_29950

MOH:	IHQ72_13665

MMEI:

LRP31_13810

MESJ:

MJ8_16210 MJ8_45140

MESS:

ABSY17_16805

MEWS:

QAZ47_14760 QAZ47_15430

MEWM:

QAZ22_14695 QAZ22_15295

PHT:	BLM14_23910

SME:

SM_b20173

SMK:	Sinme_3993

SMQ:	SinmeB_4490

SMX:	SM11_pD1430

SMI:	BN406_06484(xoxF)

SMEG:

C770_GR4pD1416

SMEL:

SM2011_b20173

SMER:

DU99_32470

SMD:	Smed_3943 Smed_6351

SFH:	SFHH103_05904

SFD:	USDA257_c30950(xoxF1) USDA257_c54310(xoxF2)

SAME:

SAMCFNEI73_Ch0092(xoxF) SAMCFNEI73_pC0377(xoxF)

SINO:

SS05631_c38640

ESJ:	SJ05684_b47680 SJ05684_b58950

EMX:	FKV68_00760 FKV68_33145

ENU:	PYH37_001447 PYH37_003874

EGI:	PZN02_001154 PZN02_004165

SKM:	PZL22_000951

STEG:

QA637_17235 QA637_25260

EAH:	FA04_27875

EAW:	ACEP9W_03870(xoxF5) ACEP9W_27745

RGA:	RGR602_PC01545

RSUL:

N2599_31080

NPM:	QEO92_19100

RHT:	NT26_3262(mxaF)

ABAW:

D5400_04045

ASIT:

N7E02_11980

BJA:	blr6213(mxaF')

BJU:	BJ6T_34880

BJP:	RN69_16920

BRA:	BRADO5484(mxaF')

BBT:	BBta_5968(mxaF')

BRS:	S23_20820(mxaF')

AOL:

S58_22240

BRC:	BCCGELA001_15250 BCCGELA001_26700

BRAD:

BF49_5268 BF49_5357 BF49_5363 BF49_5368

BIC:	LMTR13_05740 LMTR13_13665

BRO:	BRAD285_1749(mxaF) BRAD285_2933(xoxF)

BRK:	CWS35_32595

BOT:	CIT37_23165(xoxF5)

BRQ:	CIT40_23240(xoxF5)

BGQ:	X265_08495 X265_11190

BGZ:	XH91_10415

BSYM:

CIT39_22235(xoxF5)

BBET:

F8237_25290

BARH:

WN72_34680

BVZ:	BRAD3257_2934(xoxF) BRAD3257_6337(xoxF)

BEL:	BE61_38340

BDG:	LPJ38_32945

BSEP:

HAP48_0048230

BQB:	J4P68_0007440

BBAN:

J4G43_037130

BCAN:

BcanWSM471_27575 BcanWSM471_28005

BAUT:

QA635_14755

BBRA:

QA636_14485

BGK:	IC762_25695

BCOU:

IC761_10440

BPAH:

QA639_30335

BXN:	I3J27_27095

BROS:

QUH67_13110

BRAZ:

LRP30_28655

BOY:	LQG66_33960

BRAY:

ML401_23900 ML401_25075

RPE:

RPE_0851

RHOL:

RBJ75_09470

TRB:	HB776_18795

TALZ:

RPMA_04795

TARD:

RSO67_12565

XAU:	Xaut_1781 Xaut_4686

XDI:	EZH22_07465

STAR:

G3545_05010 G3545_09685

LNE:	FZC33_09215

SNO:

Snov_1035

ANC:	GBB76_01675

APRA:

G3A50_17630 G3A50_20050

APOL:

K9D25_19075

MEA:	Mex_1p1740(xoxF) Mex_1p2757(xoxF)

MDI:

METDI2492

MEX:	Mext_0099 Mext_1809

MCH:	Mchl_2145 Mchl_5444

MPO:

Mpop_1761

MZA:	B2G69_16705

METQ:

MSPGM_18470(xoxF)

MRD:	Mrad2831_0508 Mrad2831_1932

MET:	M446_2082 M446_2994 M446_5752

MNO:	Mnod_0421 Mnod_2344 Mnod_5977

MOR:	MOC_0664 MOC_2340

META:

Y590_08660 Y590_19095

MAQU:

Maq22A_c05215(xoxF1) Maq22A_c27990(xoxF2)

MPHY:

MCBMB27_00085 MCBMB27_01555

MEE:	DA075_09885 DA075_21965

METD:

C0214_10415 C0214_21230

METX:

A3862_21280 A3862_28410

METS:

DK389_01595

METI:

DK427_20455

MMES:

MMSR116_16580 MMSR116_22465

MTEA:

DK419_15175 DK419_17835

MIND:

mvi_46800 mvi_49900

MOG:	MMB17_05055 MMB17_11850 MMB17_20135(xoxF)

MTAD:

M6G65_04760 M6G65_13775

MECL:

CLZ_08180 CLZ_19040

MFUJ:

ABC766_06735(xoxF1) ABC766_08320(xoxF5) ABC766_30720

MENI:

GU700_08180 GU700_19045

MENS:

LOK46_02770 LOK46_10135

MOC:	BB934_29530

MICO:

GDR74_15460

MTEZ:

HPT29_023400

MLD:	U0023_06155 U0023_24430 U0023_26470

MSL:	Msil_1587 Msil_2260 Msil_3149 Msil_3387

MTUN:

MTUNDRAET4_0121.1(xoxF3) MTUNDRAET4_1637(xoxF1) MTUNDRAET4_3562(xoxF5) MTUNDRAET4_3771(xoxF5-2)

MLG:	CWB41_02560 CWB41_08010 CWB41_10035 CWB41_12120

MEDK:

QEV83_18970

BBAR:

RHAL1_02998(xoxF)

BBAI:

CU048_13440

HDN:	Hden_1305 Hden_1617 Hden_1869 Hden_2848

HDT:	HYPDE_23038 HYPDE_23343 HYPDE_25993 HYPDE_26383 HYPDE_29963 HYPDE_37788

HMC:	HYPMC_0800 HYPMC_1896 HYPMC_3613 HYPMC_4120

HNI:	W911_03300 W911_13290 W911_16160

FIL:	BN1229_v1_0562(xoxF) BN1229_v1_2382(xoxF)

FIY:	BN1229_v1_0564(xoxF) BN1229_v1_3533(xoxF)

MCG:	GL4_0437 GL4_1130 GL4_1360 GL4_1361

METG:

HT051_03065 HT051_11780

DRH:	JI748_03070

DEVD:

GHV40_10295 GHV40_20180

MSC:

BN69_1363

MBRY:

B1812_05555 B1812_07070

MROS:

EHO51_13105

MHEY:

H2LOC_003720 H2LOC_008405 H2LOC_017605

MPAR:

F7D14_03490 F7D14_03920 F7D14_05105

MIWA:

SS37A_00170(mxaF') SS37A_33470 SS37A_35570

MESZ:

LNB28_11565

MTW:	CQW49_14395 CQW49_15840

MECQ:

MSC49_09890(mxaF'_2) MSC49_39370(mxaF'_3) MSC49_42880

PLEO:

OHA_1_00064(xoxF1) OHA_1_01165(moxF_1) OHA_1_03539(moxF_2) OHA_1_04184(moxF_4)

HDI:	HDIA_2042(moxF) HDIA_2631

AALA:

IGS74_05660

AALM:

LUX29_06415

AUZ:	Sa4125_38750

AUB:	LXB15_00835 LXB15_14965

JAV:	OXU80_20900

NOH:	G5V57_12150

BRN:	D1F64_06495

ACUT:

MRB58_04380

MFLG:

ABS361_01650(xoxF5) ABS361_20380

RUA:	D1823_18595

RUY:	NOR97_16510

RDE:	RD1_0890(moxF)

RLI:	RLO149_c037700

RFU:	ROLI_041940(moxF)

DSH:	Dshi_0476(gcd)

YPAC:

CEW88_21790

YAN:	AYJ57_23725

TPRO:

Ga0080559_TMP1770

PABY:

Ga0080574_TMP283

RMM:	ROSMUCSMR3_01466(moxF)

ROK:	RAK1035_1700

ROH:	FIU89_13740(moxF)

SAGU:

CDO87_22695

SSTL:

ABFK29_24505(xoxF5)

OCD:	FHY55_11650

RMH:	LVO79_17305

MALU:

KU6B_56410

PALX:

GQA70_00445

RSP:	RSP_2578(xoxF)

RSH:	Rsph17029_1236

RSQ:	Rsph17025_1945

RSK:	RSKD131_0890

CAZT:

LV780_08620

PDE:

Pden_0020

PAMI:

JCM7686_0090

PYE:	A6J80_08640

PCON:

B0A89_05630

PARO:

CUV01_07535

PAMN:

JCM7685_0197

PARR:

EOJ32_02175

PAAK:

FIU66_13715

PKD:	F8A10_00235

PPAN:

ESD82_21230

PLIA:

E4191_14860

PMAU:

CP157_00045(moxF)

PMEH:

JWJ88_10740

PSEW:

JHW44_01030

PSAP:

JHX88_20910

PAEX:

JHW48_00115

PSTL:

JHW45_00490

PALP:

JHW40_18900

PVER:

E3U25_19910

PFEO:

E3U26_08185

RHC:

RGUI_0956

DAA:	AKL17_0668

HML:	HmaOT1_17500

PPRU:

FDP22_18705

PAMO:

BAR1_01250

MON:	G8E03_03635

PGV:	SL003B_1016

AMAQ:

GO499_15620

MANH:

LA6_001899(moxF)

NOG:	GKE62_05770

SCAB:

LZK98_17585

SSAA:

RPR59_09500

GBE:	GbCGDNIH1_0122 GbCGDNIH1_0651 GbCGDNIH1_1922

GBH:	GbCGDNIH2_0122 GbCGDNIH2_0651 GbCGDNIH2_1922

GBC:	GbCGDNIH3_0122 GbCGDNIH3_0651 GbCGDNIH3_1922

GBS:	GbCGDNIH4_0122 GbCGDNIH4_0651 GbCGDNIH4_1922

RAP:	RHOA_6230(xoxF)

ACR:

Acry_2790

AMV:	ACMV_31220

RMT:	IAI58_19060 IAI58_19585

ALI:	AZOLI_p10703(moxF2)

ABS:	AZOBR_50044(mxaF) AZOBR_p270106(moxF)

ABQ:	ABAZ39_14850 ABAZ39_25350

ABF:	AMK58_22450

ATI:	AL072_26995

AHU:	A6A40_28830

AZT:	TSH58p_01085

AZM:	DM194_17165

AZZ:	DEW08_25260

AARE:

D3093_25080

AZS:	E6C72_22375

AZP:	E6C67_07260

NAO:	Y958_31085

SKT:	IGS68_06280

SMUC:

JL100_021340

SROE:

JL101_020035

ACRU:

HHL28_01580

MTAR:

DF168_02132(moxF)

MIN:	Minf_0992(gcd)

MKC:	kam1_1014(gcd)

MFH:	MFUM_1183(xoxF)

MCAO:

IT6_01685

MEAP:

MTHMO_1700(xoxF1) MTHMO_1756(xoxF2)

ORP:	MOP44_12205 MOP44_26705

SUS:

Acid_6912

GBA:	J421_0297 J421_1793 J421_4070

HTH:

HTH_0392

HTE:	Hydth_0390

LPIL:

LIP_1128

MOX:	DAMO_0124(mxaF) DAMO_0134(mxaF)

» show all

Reference

1 [PMID:21256798]

Authors

Hibi Y, Asai K, Arafuka H, Hamajima M, Iwama T, Kawai K

Title

Molecular structure of La3+-induced methanol dehydrogenase-like protein in Methylobacterium radiotolerans.

Journal

J Biosci Bioeng 111:547-9 (2011)
DOI:10.1016/j.jbiosc.2010.12.017

Sequence

[mrd:Mrad2831_0508]

Reference

2 [PMID:23209751]

Authors

Nakagawa T, Mitsui R, Tani A, Sasa K, Tashiro S, Iwama T, Hayakawa T, Kawai K

Title

A catalytic role of XoxF1 as La3+-dependent methanol dehydrogenase in Methylobacterium extorquens strain AM1.

Journal

PLoS One 7:e50480 (2012)
DOI:10.1371/journal.pone.0050480

Reference

3 [PMID:24034209]

Authors

Pol A, Barends TR, Dietl A, Khadem AF, Eygensteyn J, Jetten MS, Op den Camp HJ

Title

Rare earth metals are essential for methanotrophic life in volcanic mudpots.

Journal

Environ Microbiol 16:255-64 (2014)
DOI:10.1111/1462-2920.12249

Reference

4 [PMID:25421364]

Authors

Bogart JA, Lewis AJ, Schelter EJ

Title

DFT study of the active site of the XoxF-type natural, cerium-dependent methanol dehydrogenase enzyme.

Journal

Chemistry 21:1743-8 (2015)
DOI:10.1002/chem.201405159

Reference

5 [PMID:28488399]

Authors

Prejano M, Marino T, Russo N

Title

How Can Methanol Dehydrogenase from Methylacidiphilum fumariolicum Work with the Alien Ce(III) Ion in the Active Center? A Theoretical Study.

Journal

Chemistry 23:8652-8657 (2017)
DOI:10.1002/chem.201700381

Reference

6 [PMID:29404411]

Authors

Masuda S, Suzuki Y, Fujitani Y, Mitsui R, Nakagawa T, Shintani M, Tani A.

Title

Lanthanide-Dependent Regulation of Methylotrophy in Methylobacteriumaquaticum Strain 22A.

Journal

mSphere 3:e00462-17 (2018)
DOI:10.1128/mSphere.00462-17

Other DBs

ExplorEnz - The Enzyme Database:

1.1.2.10

IUBMB Enzyme Nomenclature:

1.1.2.10

ExPASy - ENZYME nomenclature database:

1.1.2.10

BRENDA, the Enzyme Database:

1.1.2.10

DBGET integrated database retrieval system