KEGG   ENZYME: 1.1.3.2
Entry
EC 1.1.3.2                  Enzyme                                 
Name
L-lactate oxidase;
lctO (gene name);
LOX
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
Sysname
(S)-lactate:oxygen 2-oxidoreductase
Reaction(IUBMB)
(S)-lactate + O2 = pyruvate + H2O2 [RN:R11996]
Reaction(KEGG)
R11996
Substrate
(S)-lactate [CPD:C00186];
O2 [CPD:C00007]
Product
pyruvate [CPD:C00022];
H2O2 [CPD:C00027]
Comment
Contains flavin mononucleotide (FMN). The best characterized enzyme is that from the bacterium Aerococcus viridans. The enzyme is widely used in biosensors to measure the lactate concentration in blood and other tissues.
History
EC 1.1.3.2 created 1961, transferred 1972 to EC 1.13.12.4, reinstated 2018
Orthology
K10530  L-lactate oxidase
Genes
ECPECP_3788
ECIUTI89_C4909
EABECABU_c48710
ELUUM146_21760
STYSTY1444
STTt1529
SEXSTBHUCCB_16270
SENTTY21A_07760
STMSTM1620
SEOSTM14_1959
SEVSTMMW_16151
SEYSL1344_1550
SEMSTMDT12_C16390
SEJSTMUK_1589
SEBSTM474_1632
SEFUMN798_1696
SETUSTU288_04445
SETCCFSAN001921_09000
SENRSTMDT2_15431
SENDDT104_15911
SENICY43_08270
SEENSE451236_14010
SPTSPA1249
SEKSSPA1158
SPQSPAB_01660
SEISPC_2115
SECSCH_1616(gox)
SEHSeHA_C1798
SHBSU5_02232
SENHCFSAN002069_00865
SEEHSEEH1578_17345
SEESNSL254_A1734
SENNSN31241_26940
SEWSeSA_A1737
SEDSeD_A1722
SENJCFSAN001992_03435
SEEBSEEB0189_011410
SENBBN855_16660
SENEIA1_08025
SENCSEET0819_14795
SBGSBG_1447
SBZA464_1656
SBVN643_06980
CIFAL515_18955
CPARCUC49_14815
PLUplu4371
PLUMA4R40_21655
PAYPAU_03922
PTTVY86_05605
PAKHB0X70_02530
PLUICE143_02515
PMRPMI3575
PMIBBB2000_0017
PVLAOB99_02125
PVGCRN77_01925
PHAUPH4a_09260
PROTBTA34_00870
PCOLF1325_02190
PCIBF9282_02060
PPEEI6G31_07210
PSIS70_10120
PSXDR96_1174
PSTABGK56_13080
PRGRB151_000650
PRQCYG50_15725
PVCG3341_00350
PMAGJI723_00375
AETLDL57_16325
OOIA6A10_04105
PAETNCTC13378_00696(lldD)
PALKPSAKL28_31650
PSYYDLE54_00575
ALABFG52_11125
NZLD0T92_01655
USULVJ78_00590
RGUA4W93_09925
KAIK32_03900(lox)
PARUCYR75_06570
LLAL78730(lctO)
LLKLLKF_1317(lctO)
LLTCVCAS_1218(lctO)
LLSlilo_1190(lctO)
LLDP620_07105
LLXNCDO2118_1281(lctO)
LLJLG36_1149(lctO)
LLWkw2_1200(lctO)
LGRLCGT_0940
LGVLCGL_0961
LRNCMV25_08755
LACTD7I46_00845
LACKFLP15_06370
LTIJW886_07860
LPETlgb_01005(lldD)
SPYSPy_0414(lctO)
SPZM5005_Spy0340(lctO)
SPYMM1GAS476_0407(lctO)
SPYAA20_0391(lctO)
SPMspyM18_0462
SPGSpyM3_0297(lctO)
SPSSPs1560
SPHMGAS10270_Spy0339(lctO)
SPIMGAS10750_Spy0338(lctO)
SPJMGAS2096_Spy0360(lctO)
SPKMGAS9429_Spy0343(lctO)
SPFSpyM51519(lctO)
SPAM6_Spy0366
SPBM28_Spy0328(lctO)
STGMGAS15252_0366(lctO)
STXMGAS1882_0366(lctO)
SOZSpy49_0335(lctO)
STZSPYALAB49_000371(lctO)
SPYHL897_01860
SPNSP_0715(lctO-2)
SPDSPD_0621(lctO)
SPRspr0627(lctO)
SPWSPCG_0663(lctO)
SJJSPJ_0655(lctO)
SNVSPNINV200_06300(lctO)
SPXSPG_0648
SNTSPT_0731(lctO)
SNDMYY_0751
SPNNT308_03345
SNESPN23F06390(lctO)
SNCHMPREF0837_10999(lctO)
SNMSP70585_0761(lctO)
SPPSPP_0725(lctO)
SNIINV104_05940(lctO)
SPNGHMPREF1038_00731(lctO)
SNBSP670_0763(lctO)
SNPSPAP_0691
SNXSPNOXC06470(lctO)
SNUSPNA45_01046(lctO)
SPNESPN034156_16960(lctO)
SPNUSPN034183_06480(lctO)
SPNMSPN994038_06370(lctO)
SPNOSPN994039_06380(lctO)
SDSSDEG_0467
SDGSDE12394_02255
SDAGGS_0455(lctO)
SDCSDSE_0485(lctO)
SDQSDSE167_0510 SDSE167_0688
SMBsmi_1435(lctO)
SORSOR_1410(lctO)
STDSPPN_03650
SANCSANR_1809(lctO)
SANSDK43_01600
SOII872_06565
SIKK710_1519
SIQDQ08_07070
SIODW64_07055
SIZSI82_07180
STVV470_06835
STRNSNAG_1378
SSOBDK181_01730
SPEIEHW89_07810(lctO)
SGWD7D53_01845(lctO)
SPLRC0J00_07810(lctO)
SCAINCTC12191_00095
SPSUNCTC13786_01762
SPOCNCTC10925_01392
SLATJ4854_08570(lctO)
SACOSAME_01893
FPENKIW23_05420
LJOLJ_1826
LJFFI9785_1781(lox)
LJHLJP_1765c
LJNT285_09015
LDELDBND_1877
LGALGAS_1864
LCRLCRIS_01388(lctO)
LAMLA2_10015
LAILAC30SC_09890
LAYLAB52_08940
LAWLACWKB8_1567
LJEBUE77_00875
LPWLpgJCM5343_17890(lldD)
LKLDKL58_08065
LAPIDKL56_06925
LHSDLD54_07475
LTAH1A07_09050
LUUH4B44_09870
LPAIGYM71_08955
LIQKBW87_07245
LCALSEI_2339
LCZLCAZH_0657 LCAZH_2316
LCSLCBD_0793 LCBD_2528
LCELC2W_0792 LC2W_2509
LCWBN194_07870(GLO5) BN194_24790(haox)
LCLLOCK919_0819 LOCK919_2578
LPQAF91_02630 AF91_11630
LPILBPG_02265 LBPG_02784
LPAPLBPC_0638 LBPC_2269
LCBLCABL_07830(loxL) LCABL_25240(loxL)
LCXLCA12A_1745 LCA12A_1863
LRHLGG_00707(lctO) LGG_02356(lctO)
LRGLRHM_0684 LRHM_2266
LRLLC705_00676(lctO) LC705_02348(lctO)
LRALRHK_2358 LRHK_697
LROLOCK900_0647 LOCK900_2318
LRCLOCK908_0692
LCHIKG086_10370
LZEKG087_03350
LPLlp_3586(lox)
LPJJDM1_2867(lox)
LPTzj316_0187(lox)
LPSLPST_C2930(lox)
LPRLBP_cg2908
LPZLp16_2802
LPBSH83_14840
LPXASU28_14350
LGASLG045_04540
LNGBSQ50_08940
LHWBSQ49_03680 BSQ49_10455
LMALLM596_03935 LM596_10755
LBHLbuc_0007
LBNLBUCD034_0007(lctO)
LPARFAM21731_01021
LCUPL11_003900
LKFDNL43_05070
LHILG8J22_00007
LLAOLHL71_06325
LACALAC1533_0078 LAC1533_0406
LBTAYR52_01550
LHOLOOC260_112600
LPDAYR62_01700
PPEPEPE_0922
PPENT256_04505
PACIA4V11_02510
LROSLROSL1_2171
LGNABM34_03100
LHIJP39_04060
LALWBTM29_09370
LALILA20249_01195
LFMLF20184_08415
LZHD1B17_04365
LFTFG051_08220
CPABG6534_03395
LSALCA_1399(loxL2)
LCVFBA2_03430 FBA2_07055
LGMLG542_06315
LAHLA20533_03160
LHBD1010_00615
LDXLH506_01225
LPSEFGL85_07520
WCEWS08_0532
WCTWS74_0533
WCIWS105_0530
WCBAO080_07710
WJOFOL01_1973
WPACO680_08405
WCFC6P13_04965
WHEEFA59_05015
WEIEQG49_00790
EFCEFAU004_02122 EFAU004_02455
EFAUEFAU085_02150 EFAU085_02535
EFUHMPREF0351_12117 HMPREF0351_12395(lctO)
EFMM7W_2415 M7W_886
EFTM395_11400
EHREHR_08130
ECASECBG_02725
EMUEMQU_2062
EDULIU_11425
EGAAL523_00710
ETHCK496_10930
EGVEGCR1_02730 EGCR1_03435
EAVEH197_12015 EH197_12975
ESGEsVE80_24370(lctO)
ELACI4Q40_03645 I4Q40_11440
ERAFJ9537_05185 J9537_05840
EIEENLAB_09720(lldD)
THLTEH_25400(lctO)
TEYGLW17_06900
TOOC7K38_04480
TKRC7K43_10630
VTEBHY08_09945
VFVK5K99_01725
AURHMPREF9243_1873
AUNAWM73_00660
AUIAPT62_00580 APT62_01510
ASANAWM72_04090
AVSAWM76_10020
AUHAWM75_00100
CRNCAR_c06050(loxL) CAR_c11800(haox)
CAWQ783_02590 Q783_05440
CARCNY10_1308
CARNFPV25_03910(lctO)
CVDLHA31_03975 LHA31_08715
MARRBKP56_03985
JEPBW721_05245
JDABW727_101663(hmo)
JEHEJN90_10585
JARG7057_08965
JPOG7058_07150
DPMFNV33_00335
AINAcin_0614
EBMSG0102_17560(lctO)
NIEKV110_26345
SIJSCIP_1210
MYRMYRA21_0969(lldD)
MPWMPR_3501
MODAS202_04480
MYZBK054_04375
 » show all
Reference
1  [PMID:2818595]
  Authors
Duncan JD, Wallis JO, Azari MR
  Title
Purification and properties of Aerococcus viridans lactate oxidase.
  Journal
Biochem Biophys Res Commun 164:919-26 (1989)
DOI:10.1016/0006-291X(89)91546-5
Reference
2  [PMID:8589073]
  Authors
Maeda-Yorita K, Aki K, Sagai H, Misaki H, Massey V
  Title
L-lactate oxidase and L-lactate monooxygenase: mechanistic variations on a common structural theme.
  Journal
Biochimie 77:631-42 (1995)
DOI:10.1016/0300-9084(96)88178-8
  Sequence
Reference
3  [PMID:10508058]
  Authors
Gibello A, Collins MD, Dominguez L, Fernandez-Garayzabal JF, Richardson PT
  Title
Cloning and analysis of the L-lactate utilization genes from Streptococcus iniae.
  Journal
Appl Environ Microbiol 65:4346-50 (1999)
DOI:10.1128/AEM.65.10.4346-4350.1999
  Sequence
[sik:K710_1519]
Reference
4  [PMID:17007814]
  Authors
Umena Y, Yorita K, Matsuoka T, Kita A, Fukui K, Morimoto Y
  Title
The crystal structure of L-lactate oxidase from Aerococcus viridans at 2.1A resolution reveals the mechanism of strict substrate recognition.
  Journal
Biochem Biophys Res Commun 350:249-56 (2006)
DOI:10.1016/j.bbrc.2006.09.025
  Sequence
Reference
5  [PMID:18367206]
  Authors
Furuichi M, Suzuki N, Dhakshnamoorhty B, Minagawa H, Yamagishi R, Watanabe Y, Goto Y, Kaneko H, Yoshida Y, Yagi H, Waga I, Kumar PK, Mizuno H
  Title
X-ray structures of Aerococcus viridans lactate oxidase and its complex with D-lactate at pH 4.5 show an alpha-hydroxyacid oxidation mechanism.
  Journal
J Mol Biol 378:436-46 (2008)
DOI:10.1016/j.jmb.2008.02.062
  Sequence
Reference
6  [PMID:27302031]
  Authors
Stoisser T, Brunsteiner M, Wilson DK, Nidetzky B
  Title
Conformational flexibility related to enzyme activity: evidence for a dynamic active-site gatekeeper function of Tyr(215) in Aerococcus viridans lactate oxidase.
  Journal
Sci Rep 6:27892 (2016)
DOI:10.1038/srep27892
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.3.2
IUBMB Enzyme Nomenclature: 1.1.3.2
ExPASy - ENZYME nomenclature database: 1.1.3.2
BRENDA, the Enzyme Database: 1.1.3.2

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