KEGG   ENZYME: 1.1.3.2
Entry
EC 1.1.3.2                  Enzyme                                 
Name
L-lactate oxidase;
lctO (gene name);
LOX
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
Sysname
(S)-lactate:oxygen 2-oxidoreductase
Reaction(IUBMB)
(S)-lactate + O2 = pyruvate + H2O2 [RN:R11996]
Reaction(KEGG)
R11996
Substrate
(S)-lactate [CPD:C00186];
O2 [CPD:C00007]
Product
pyruvate [CPD:C00022];
H2O2 [CPD:C00027]
Comment
Contains flavin mononucleotide (FMN). The best characterized enzyme is that from the bacterium Aerococcus viridans. The enzyme is widely used in biosensors to measure the lactate concentration in blood and other tissues.
History
EC 1.1.3.2 created 1961, transferred 1972 to EC 1.13.12.4, reinstated 2018
Orthology
K10530  L-lactate oxidase
Genes
ECP: ECP_3788
ECI: UTI89_C4909
EAB: ECABU_c48710
ELU: UM146_21760
STY: STY1444
STT: t1529
SEX: STBHUCCB_16270
SENT: TY21A_07760
STM: STM1620
SEO: STM14_1959
SEV: STMMW_16151
SEY: SL1344_1550
SEM: STMDT12_C16390
SEJ: STMUK_1589
SEB: STM474_1632
SEF: UMN798_1696
SETU: STU288_04445
SETC: CFSAN001921_09000
SENR: STMDT2_15431
SEND: DT104_15911
SENI: CY43_08270
SEEN: SE451236_14010
SPT: SPA1249
SEK: SSPA1158
SPQ: SPAB_01660
SEI: SPC_2115
SEC: SCH_1616(gox)
SEH: SeHA_C1798
SHB: SU5_02232
SENH: CFSAN002069_00865
SEEH: SEEH1578_17345
SEE: SNSL254_A1734
SENN: SN31241_26940
SEW: SeSA_A1737
SED: SeD_A1722
SENJ: CFSAN001992_03435
SEEB: SEEB0189_011410
SENB: BN855_16660
SENE: IA1_08025
SENC: SEET0819_14795
SBG: SBG_1447
SBZ: A464_1656
SBV: N643_06980
CIF: AL515_18955
CPAR: CUC49_14815
PLU: plu4371
PLUM: A4R40_21655
PAY: PAU_03922
PTT: VY86_05605
PAKH: B0X70_02530
PLUI: CE143_02515
PMR: PMI3575
PMIB: BB2000_0017
PVL: AOB99_02125
PVG: CRN77_01925
PHAU: PH4a_09260
PROT: BTA34_00870
PCOL: F1325_02190
PCIB: F9282_02060
PPEE: I6G31_07210
PAPP: QQS39_02360
PSI: S70_10120
PSX: DR96_1174
PSTA: BGK56_13080
PTHA: OI982_13080
PRG: RB151_000650
PRQ: CYG50_15725
PVC: G3341_00350
PMAG: JI723_00375
AET: LDL57_16325
AAPC: QG404_00345
OOI: A6A10_04105
PAET: NCTC13378_00696(lldD)
PALK: PSAKL28_31650
PKK: QQ992_14980
PSYY: DLE54_00575
PRAE: MN210_17505
ALA: BFG52_11125
NZL: D0T92_01655
USU: LVJ78_00590
APES: FOC84_29945
ROI: N4261_15785
RGU: A4W93_09925
DZO: SR858_13940
OVB: NB640_01760
KAI: K32_03900(lox)
PARU: CYR75_06570
PSTL: JHW45_15615 JHW45_17030
VIK: KFZ58_05885
LLA: L78730(lctO)
LLK: LLKF_1317(lctO)
LLT: CVCAS_1218(lctO)
LLS: lilo_1190(lctO)
LLD: P620_07105
LLX: NCDO2118_1281(lctO)
LLJ: LG36_1149(lctO)
LLW: kw2_1200(lctO)
LGR: LCGT_0940
LGV: LCGL_0961
LRN: CMV25_08755
LACT: D7I46_00845
LACK: FLP15_06370
LTI: JW886_07860
LPET: lgb_01005(lldD)
LFO: LMK00_06085
SPY: SPy_0414(lctO)
SPZ: M5005_Spy0340(lctO)
SPYM: M1GAS476_0407(lctO)
SPYA: A20_0391(lctO)
SPM: spyM18_0462
SPG: SpyM3_0297(lctO)
SPS: SPs1560
SPH: MGAS10270_Spy0339(lctO)
SPI: MGAS10750_Spy0338(lctO)
SPJ: MGAS2096_Spy0360(lctO)
SPK: MGAS9429_Spy0343(lctO)
SPF: SpyM51519(lctO)
SPA: M6_Spy0366
SPB: M28_Spy0328(lctO)
STG: MGAS15252_0366(lctO)
STX: MGAS1882_0366(lctO)
SOZ: Spy49_0335(lctO)
STZ: SPYALAB49_000371(lctO)
SPYH: L897_01860
SPN: SP_0715(lctO-2)
SPD: SPD_0621(lctO)
SPR: spr0627(lctO)
SPW: SPCG_0663(lctO)
SJJ: SPJ_0655(lctO)
SNV: SPNINV200_06300(lctO)
SPX: SPG_0648
SNT: SPT_0731(lctO)
SND: MYY_0751
SPNN: T308_03345
SNE: SPN23F06390(lctO)
SNC: HMPREF0837_10999(lctO)
SNM: SP70585_0761(lctO)
SPP: SPP_0725(lctO)
SNI: INV104_05940(lctO)
SPNG: HMPREF1038_00731(lctO)
SNB: SP670_0763(lctO)
SNP: SPAP_0691
SNX: SPNOXC06470(lctO)
SNU: SPNA45_01046(lctO)
SPNE: SPN034156_16960(lctO)
SPNU: SPN034183_06480(lctO)
SPNM: SPN994038_06370(lctO)
SPNO: SPN994039_06380(lctO)
SDS: SDEG_0467
SDG: SDE12394_02255
SDA: GGS_0455(lctO)
SDC: SDSE_0485(lctO)
SDQ: SDSE167_0510 SDSE167_0688
SMB: smi_1435(lctO)
SOR: SOR_1410(lctO)
STD: SPPN_03650
SANC: SANR_1809(lctO)
SANS: DK43_01600
SOI: I872_06565
SIK: K710_1519
SIQ: DQ08_07070
SIO: DW64_07055
SIZ: SI82_07180
STV: V470_06835
STRN: SNAG_1378
SSOB: DK181_01730
SPEI: EHW89_07810(lctO)
SGW: D7D53_01845(lctO)
SPLR: C0J00_07810(lctO)
SCAI: NCTC12191_00095
SPSU: NCTC13786_01762
SPOC: NCTC10925_01392
SLAT: J4854_08570(lctO)
SACO: SAME_01893
STOY: STYK_13950(lctO)
SMIE: NCTC11169_01640(lctO)
SPAM: SP4011_15950(lctO)
FPEN: KIW23_05420
LJO: LJ_1826
LJF: FI9785_1781(lox)
LJH: LJP_1765c
LJN: T285_09015
LDE: LDBND_1877
LGA: LGAS_1864
LCR: LCRIS_01388(lctO)
LAM: LA2_10015
LAI: LAC30SC_09890
LAY: LAB52_08940
LAW: LACWKB8_1567
LJE: BUE77_00875
LPW: LpgJCM5343_17890(lldD)
LKL: DKL58_08065
LAPI: DKL56_06925
LHS: DLD54_07475
LTA: H1A07_09050
LUU: H4B44_09870
LPAI: GYM71_08955
LIQ: KBW87_07245
LMUL: PUW59_09005
LXO: KIM322_13250(lox)
LCA: LSEI_2339
LCZ: LCAZH_0657 LCAZH_2316
LCS: LCBD_0793 LCBD_2528
LCE: LC2W_0792 LC2W_2509
LCW: BN194_07870(GLO5) BN194_24790(haox)
LCL: LOCK919_0819 LOCK919_2578
LPQ: AF91_02630 AF91_11630
LPI: LBPG_02265 LBPG_02784
LPAP: LBPC_0638 LBPC_2269
LCB: LCABL_07830(loxL) LCABL_25240(loxL)
LCX: LCA12A_1745 LCA12A_1863
LRH: LGG_00707(lctO) LGG_02356(lctO)
LRG: LRHM_0684 LRHM_2266
LRL: LC705_00676(lctO) LC705_02348(lctO)
LRA: LRHK_2358 LRHK_697
LRO: LOCK900_0647 LOCK900_2318
LRC: LOCK908_0692
LCHI: KG086_10370
LZE: KG087_03350
LHN: LHUE1_000448 LHUE1_001785
LPAB: PQ472_00105
LPAH: LACPH_000653 LACPH_002190
LSB: LACSTY_000614 LACSTY_002267
LPL: lp_3586(lox)
LPJ: JDM1_2867(lox)
LPT: zj316_0187(lox)
LPS: LPST_C2930(lox)
LPR: LBP_cg2908
LPZ: Lp16_2802
LPB: SH83_14840
LPX: ASU28_14350
LGAS: LG045_04540
LNG: BSQ50_08940
LHW: BSQ49_03680 BSQ49_10455
LMAL: LM596_03935 LM596_10755
LBH: Lbuc_0007
LBN: LBUCD034_0007(lctO)
LPAR: FAM21731_01021
LCU: PL11_003900
LKF: DNL43_05070
LHIL: G8J22_00007
LLAO: LHL71_06325
LACA: LAC1533_0078 LAC1533_0406
LBT: AYR52_01550
LHO: LOOC260_112600
LPD: AYR62_01700
PPE: PEPE_0922
PPEN: T256_04505
PACI: A4V11_02510
LROS: LROSL1_2171
LGN: ABM34_03100
LHI: JP39_04060
LALW: BTM29_09370
LALI: LA20249_01195
LFM: LF20184_08415
LZH: D1B17_04365
LFT: FG051_08220
CPAB: G6534_03395
LSA: LCA_1399(loxL2)
LCV: FBA2_03430 FBA2_07055
LGM: LG542_06315
LAH: LA20533_03160
LHB: D1010_00615
LDX: LH506_01225
LPSE: FGL85_07520
WCE: WS08_0532
WTR: KHQ32_02925
WCT: WS74_0533
WCI: WS105_0530
WCB: AO080_07710
WJO: FOL01_1973
WPA: CO680_08405
WCF: C6P13_04965
WHE: EFA59_05015
WEI: EQG49_00790
EFC: EFAU004_02122 EFAU004_02455
EFAU: EFAU085_02150 EFAU085_02535
EFU: HMPREF0351_12117 HMPREF0351_12395(lctO)
EFM: M7W_2415 M7W_886
EFT: M395_11400
EHR: EHR_08130
ECAS: ECBG_02725
EMU: EMQU_2062
EDU: LIU_11425
EGA: AL523_00710
ETH: CK496_10930
EGV: EGCR1_02730 EGCR1_03435
EAV: EH197_12015 EH197_12975
ESG: EsVE80_24370(lctO)
ELAC: I4Q40_03645 I4Q40_11440
ERAF: J9537_05185 J9537_05840
EIE: ENLAB_09720(lldD)
EDS: PML78_08590
EMS: P3T75_00895
ENI: CXM95_13765
THL: TEH_25400(lctO)
TEY: GLW17_06900
TOO: C7K38_04480
TKR: C7K43_10630
VTE: BHY08_09945
VFV: K5K99_01725
VLC: G314FT_06240(hmo)
AUR: HMPREF9243_1873
AUN: AWM73_00660
AUI: APT62_00580 APT62_01510
ASAN: AWM72_04090
AVS: AWM76_10020
AUH: AWM75_00100
ALOY: CJ190_000840
AMIT: DBT49_0000895
ATJ: DBT50_006895
GSG: CYJ72_004915
FCT: NRE15_09430
CRN: CAR_c06050(loxL) CAR_c11800(haox)
CAW: Q783_02590 Q783_05440
CARC: NY10_1308
CARN: FPV25_03910(lctO)
CVD: LHA31_03975 LHA31_08715
MARR: BKP56_03985
JEP: BW721_05245
JDA: BW727_101663(hmo)
JEH: EJN90_10585
JAR: G7057_08965
JPO: G7058_07150
DPM: FNV33_00335
DST: VUQ06_04150
DINC: QNK01_04850
AIN: Acin_0614
EBM: SG0102_17560(lctO)
NIE: KV110_26345
NYU: D7D52_36365
NAKA: H7F38_25045
SIJ: SCIP_1210
MYR: MYRA21_0969(lldD)
MPW: MPR_3501
MOD: AS202_04480
MYZ: BK054_04375
 » show all
Reference
1  [PMID:2818595]
  Authors
Duncan JD, Wallis JO, Azari MR
  Title
Purification and properties of Aerococcus viridans lactate oxidase.
  Journal
Biochem Biophys Res Commun 164:919-26 (1989)
DOI:10.1016/0006-291X(89)91546-5
Reference
2  [PMID:8589073]
  Authors
Maeda-Yorita K, Aki K, Sagai H, Misaki H, Massey V
  Title
L-lactate oxidase and L-lactate monooxygenase: mechanistic variations on a common structural theme.
  Journal
Biochimie 77:631-42 (1995)
DOI:10.1016/0300-9084(96)88178-8
  Sequence
[avs:AWM76_10020]
Reference
3  [PMID:10508058]
  Authors
Gibello A, Collins MD, Dominguez L, Fernandez-Garayzabal JF, Richardson PT
  Title
Cloning and analysis of the L-lactate utilization genes from Streptococcus iniae.
  Journal
Appl Environ Microbiol 65:4346-50 (1999)
DOI:10.1128/AEM.65.10.4346-4350.1999
  Sequence
[sik:K710_1519]
Reference
4  [PMID:17007814]
  Authors
Umena Y, Yorita K, Matsuoka T, Kita A, Fukui K, Morimoto Y
  Title
The crystal structure of L-lactate oxidase from Aerococcus viridans at 2.1A resolution reveals the mechanism of strict substrate recognition.
  Journal
Biochem Biophys Res Commun 350:249-56 (2006)
DOI:10.1016/j.bbrc.2006.09.025
  Sequence
[avs:AWM76_10020]
Reference
5  [PMID:18367206]
  Authors
Furuichi M, Suzuki N, Dhakshnamoorhty B, Minagawa H, Yamagishi R, Watanabe Y, Goto Y, Kaneko H, Yoshida Y, Yagi H, Waga I, Kumar PK, Mizuno H
  Title
X-ray structures of Aerococcus viridans lactate oxidase and its complex with D-lactate at pH 4.5 show an alpha-hydroxyacid oxidation mechanism.
  Journal
J Mol Biol 378:436-46 (2008)
DOI:10.1016/j.jmb.2008.02.062
  Sequence
[avs:AWM76_10020]
Reference
6  [PMID:27302031]
  Authors
Stoisser T, Brunsteiner M, Wilson DK, Nidetzky B
  Title
Conformational flexibility related to enzyme activity: evidence for a dynamic active-site gatekeeper function of Tyr(215) in Aerococcus viridans lactate oxidase.
  Journal
Sci Rep 6:27892 (2016)
DOI:10.1038/srep27892
  Sequence
[avs:AWM76_10020]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.3.2
IUBMB Enzyme Nomenclature: 1.1.3.2
ExPASy - ENZYME nomenclature database: 1.1.3.2
BRENDA, the Enzyme Database: 1.1.3.2

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Chemical substance (4)   
   KEGG COMPOUND (4)   
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   KEGG REACTION (1)   
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Gene (1137)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (373)   
   KEGG MGENES (749)   
   RefGene (14)   
Protein sequence (14)   
   PDBSTR (14)   
3D Structure (3)   
   PDB (3)   
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