Acting on the CH-OH group of donors;
With oxygen as acceptor
thiamine + 2 O2 + H2O = thiamine acetic acid + 2 H2O2 [RN:
A flavoprotein (FAD). The product differs from thiamine in replacement of -CH2.CH2.OH by -CH2.COOH; the two-step oxidation proceeds without the release of the intermediate aldehyde from the enzyme.
EC 184.108.40.206 created 1984
Edmondson DE, Kenney WC, Singer TP.
Structural elucidation and properties of 8alpha-(N1-histidyl)riboflavin: the flavin component of thiamine dehydrogenase and beta-cyclopiazonate oxidocyclase.
Gomez-Moreno C, Edmondson DE.
Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase.
Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2'-methyl-4'-amino-5'-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism.
J Biol Chem 245:2599-604 (1970)
ExplorEnz - The Enzyme Database: 220.127.116.11
ExPASy - ENZYME nomenclature database: 18.104.22.168