Entry |
|
Name |
5-(hydroxymethyl)furfural oxidase
|
Class |
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
|
Sysname |
5-(hydroxymethyl)furfural:oxygen oxidoreductase
|
Reaction(IUBMB) |
5-(hydroxymethyl)furfural + 3 O2 + 2 H2O = furan-2,5-dicarboxylate + 3 H2O2 (overall reaction) [RN: R10858];
(1a) 5-(hydroxymethyl)furfural + O2 = furan-2,5-dicarbaldehyde + H2O2 [RN: R10853];
(1b) furan-2,5-dicarbaldehyde + H2O = 5-(dihydroxymethyl)furan-2-carbaldehyde (spontaneous) [RN: R10854];
(1c) 5-(dihydroxymethyl)furan-2-carbaldehyde + O2 = 5-formylfuran-2-carboxylate + H2O2 [RN: R10855];
(1d) 5-formylfuran-2-carboxylate + H2O = 5-(dihydroxymethyl)furan-2-carboxylate (spontaneous) [RN: R10856];
(1e) 5-(dihydroxymethyl)furan-2-carboxylate + O2 = furan-2,5-dicarboxylate + H2O2 [RN: R10857]
|
Reaction(KEGG) |
|
Substrate |
5-hydroxymethylfurfural [CPD: C11101];
O2 [CPD: C00007];
H2O [CPD: C00001];
furan-2,5-dicarbaldehyde [CPD: C20899];
5-(dihydroxymethyl)furan-2-carbaldehyde [CPD: C20900];
5-formylfuran-2-carboxylate [CPD: C20449];
5-(dihydroxymethyl)furan-2-carboxylate [CPD: C20901]
|
Product |
furan-2,5-dicarboxylate [CPD: C20450];
H2O2 [CPD: C00027];
furan-2,5-dicarbaldehyde [CPD: C20899];
5-(dihydroxymethyl)furan-2-carbaldehyde [CPD: C20900];
5-formylfuran-2-carboxylate [CPD: C20449];
5-(dihydroxymethyl)furan-2-carboxylate [CPD: C20901]
|
Comment |
The enzyme, characterized from the bacterium Methylovorus sp. strain MP688, is involved in the degradation and detoxification of 5-(hydroxymethyl)furfural. The enzyme acts only on alcohol groups and requires the spontaneous hydration of aldehyde groups for their oxidation [3]. The enzyme has a broad substrate range that overlaps with EC 1.1.3.7, aryl-alcohol oxidase.
|
History |
EC 1.1.3.47 created 2014
|
Pathway |
ec01120 | Microbial metabolism in diverse environments |
|
Orthology |
K16873 | 5-(hydroxymethyl)furfural/furfural oxidase |
|
Genes |
» show all
|
Reference |
|
Authors |
Koopman F, Wierckx N, de Winde JH, Ruijssenaars HJ |
Title |
Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14. |
Journal |
|
Sequence |
|
Reference |
|
Authors |
Dijkman WP, Fraaije MW |
Title |
Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688. |
Journal |
|
Sequence |
|
Reference |
|
Authors |
Dijkman WP, Groothuis DE, Fraaije MW |
Title |
Enzyme-catalyzed oxidation of 5-hydroxymethylfurfural to furan-2,5-dicarboxylic acid. |
Journal |
|
Other DBs |
ExplorEnz - The Enzyme Database: | 1.1.3.47 |
ExPASy - ENZYME nomenclature database: | 1.1.3.47 |
|