KEGG   ENZYME: 1.1.3.8
Entry
EC 1.1.3.8                  Enzyme                                 

Name
L-gulonolactone oxidase;
L-gulono-gamma-lactone: O2 oxidoreductase;
L-gulono-gamma-lactone oxidase;
L-gulono-gamma-lactone:oxidoreductase;
GLO
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
Sysname
L-gulono-1,4-lactone:oxygen 3-oxidoreductase
Reaction(IUBMB)
L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2 (overall reaction) [RN:R10053];
(1a) L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 [RN:R03184];
(1b) L-xylo-hex-2-ulono-1,4-lactone = L-ascorbate (spontaneous) [RN:R00647]
Reaction(KEGG)
Substrate
L-gulono-1,4-lactone [CPD:C01040];
O2 [CPD:C00007];
L-xylo-hex-2-ulono-1,4-lactone [CPD:C03289]
Product
L-ascorbate [CPD:C00072];
H2O2 [CPD:C00027];
L-xylo-hex-2-ulono-1,4-lactone [CPD:C03289]
Comment
A microsomal flavoprotein (FAD). The product spontaneously isomerizes to L-ascorbate. While most higher animals can synthesize asborbic acid, primates and guinea pigs cannot [3].
History
EC 1.1.3.8 created 1965, modified 2001, modified 2006
Pathway
ec00053  Ascorbate and aldarate metabolism
ec01100  Metabolic pathways
Orthology
K00103  L-gulonolactone oxidase
Genes
MMUR: 105877128
MMU: 268756(Gulo)
MCAL: 110308887
MPAH: 110325590
RNO: 60671(Gulo)
MCOC: 116084654
MUN: 110559217
CGE: 100760306
PLEU: 114707320
NGI: 103730028
HGL: 101717756
CCAN: 109692534
OCU: 100341843
OPI: 101534528
TUP: 102480281
CFA: 486100
VVP: 112926784
VLG: 121498164
AML: 100478547
UMR: 103675989
UAH: 113270039
ORO: 101374448
ELK: 111155991
MPUF: 101690348
EJU: 114217386
FCA: 101092602
PYU: 121022590
PBG: 122473886
PTG: 102964688
PPAD: 109262342
AJU: 106973111
HHV: 120220294
BTA: 286812(GULO)
BOM: 102268085
BIU: 109562556
BBUB: 102410395
CHX: 102186117
OAS: 101106395
CCAD: 122432065
SSC: 396759(GULO)
CFR: 102517517
CBAI: 105063867
CDK: 105102219
BACU: 103015173
LVE: 103082082
OOR: 101282721
DLE: 111185404
PCAD: 102976415
ECB: 100060483
EPZ: 103550421
EAI: 106832515
HAI: 109379502(GULO)
PALE: 102892731
PGIG: 120613175
RAY: 107510766
MJV: 108394330
TOD: 119258270
LAV: 100658302
TMU: 101343971
MDO: 100030569
SHR: 100931979
PCW: 110206533
OAA: 100093190
GGA: 770996
PCOC: 116235640
MGP: 100548691
CJO: 107312420
NMEL: 110396328
APLA: 101802453
ACYG: 106049515
TGU: 115494645
LSR: 110477408
PMOA: 120510390
OTC: 121341613
FAB: 101806211
PHI: 102114090
CCAE: 111927479
FPG: 101922774
FCH: 102048039
CLV: 102086252
EGZ: 104132031
NNI: 104014769
ACUN: 113478833
PADL: 103917790
AAM: 106486384
AROW: 112966665
NPD: 112948905
DNE: 112993190
ASN: 102383993
AMJ: 102559022
CPOO: 109311561
GGN: 109300224
PSS: 102452718
CPIC: 101948999
TST: 117871397
CABI: 116837372
ACS: 100564818
PVT: 110089492
PBI: 103058013
PMUR: 107295744
TSR: 106552982
PGUT: 117667687
PMUA: 114594945
ZVI: 118082815
GJA: 107110087
XLA: 495407
XTR: 101733882
NPR: 108796920
LOC: 102697922
PSPA: 121317358
ARUT: 117410447
CMK: 103191462
RTP: 109916960
CIN: 100180753
SKO: 100368812
LCQ: 111689991
CSEC: 111874443
EAF: 111704487
DSV: 119433475
RSAN: 119404685
RMP: 119181904
DPTE: 113788500
PTEP: 107443895
SDM: 118196990
PCAN: 112559251
BGT: 106064532
GAE: 121381575
LAK: 106161198
EPA: 110254170
ATEN: 116306443
DGT: 114518388
AQU: 100633693
ATH: AT1G32300(GulLO1) AT2G46740(GulLO5) AT2G46750(GulLO2) AT2G46760(GulLO6) AT5G11540(GulLO3) AT5G56470(GulLO7) AT5G56490(GulLO4)
LJA: Lj1g3v5060530.1(Lj1g3v5060530.1) Lj1g3v5060540.1(Lj1g3v5060540.1) Lj2g3v3250000.1(Lj2g3v3250000.1) Lj3g3v0462020.1(Lj3g3v0462020.1) Lj3g3v0462290.1(Lj3g3v0462290.1) Lj3g3v2483050.1(Lj3g3v2483050.1)
CSV: 101221035
CMO: 103483108
BHJ: 120073810
MCHA: 111013948
CMAX: 111474382
CMOS: 111448536
CPEP: 111800661
JCU: 105643326
CCAV: 112523679
DOSA: Os01t0687800-00(Os01g0687800) Os02t0124400-01(Os02g0124400) Os02t0124600-00(Os02g0124600) Os03t0563100-01(Os03g0563100) Os04t0360500-01(Os04g0360500) Os04t0361500-00(Os04g0361500) Os08t0114300-01(Os08g0114300) Os08t0519100-01(Os08g0519100)
MGR: MGG_02420
CMT: CCM_00852
MBE: MBM_05135
CNE: CNN01640
CNB: CNBN1590
TASA: A1Q1_02359
ABP: AGABI1DRAFT115961(AGABI1DRAFT_115961)
ABV: AGABI2DRAFT195196(AGABI2DRAFT_195196)
MGL: MGL_0958
MRT: MRET_3192
VAQ: FIV01_07260(xyoA)
HCH: HCH_04675
CYT: cce_4306
 » show all
Reference
1  [PMID:14405898]
  Authors
ISHERWOOD FA, MAPSON LW, CHEN YT.
  Title
Synthesis of L-ascorbic acid in rat-liver homogenates. Conversion of L-gulono- and L-galactono-gamma-lactone and the respective acids into L-ascorbic acid.
  Journal
Biochem J 76:157-71 (1960)
DOI:10.1042/bj0760157
Reference
2  [PMID:7138847]
  Authors
Kiuchi K, Nishikimi M, Yagi K.
  Title
Purification and characterization of L-gulonolactone oxidase from chicken kidney microsomes.
  Journal
Biochemistry 21:5076-82 (1982)
DOI:10.1021/bi00263a035
Reference
3  [PMID:8175804]
  Authors
Nishikimi M, Fukuyama R, Minoshima S, Shimizu N, Yagi K.
  Title
Cloning and chromosomal mapping of the human nonfunctional gene for L-gulono-gamma-lactone oxidase, the enzyme for L-ascorbic acid biosynthesis missing in man.
  Journal
J Biol Chem 269:13685-8 (1994)
Reference
4
  Authors
Chatterjee, I.B., Chatterjee, G.C., Ghosh, N.C. and Guha, B.C.
  Title
Identification of 2-keto-L-gulonolactone as an intermediate in the biosynthesis of L-ascorbic acid.
  Journal
Naturwissenschaften 46:475 (1959)
Other DBs
ExplorEnz - The Enzyme Database: 1.1.3.8
IUBMB Enzyme Nomenclature: 1.1.3.8
ExPASy - ENZYME nomenclature database: 1.1.3.8
BRENDA, the Enzyme Database: 1.1.3.8
CAS: 9028-78-8

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