KEGG   ENZYME: 1.1.3.8
Entry
EC 1.1.3.8                  Enzyme                                 
Name
L-gulonolactone oxidase;
L-gulono-gamma-lactone: O2 oxidoreductase;
L-gulono-gamma-lactone oxidase;
L-gulono-gamma-lactone:oxidoreductase;
GLO
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
Sysname
L-gulono-1,4-lactone:oxygen 3-oxidoreductase
Reaction(IUBMB)
L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2 (overall reaction) [RN:R10053];
(1a) L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 [RN:R03184];
(1b) L-xylo-hex-2-ulono-1,4-lactone = L-ascorbate (spontaneous) [RN:R00647]
Reaction(KEGG)
Substrate
L-gulono-1,4-lactone [CPD:C01040];
O2 [CPD:C00007];
L-xylo-hex-2-ulono-1,4-lactone [CPD:C03289]
Product
L-ascorbate [CPD:C00072];
H2O2 [CPD:C00027];
L-xylo-hex-2-ulono-1,4-lactone [CPD:C03289]
Comment
A microsomal flavoprotein (FAD). The product spontaneously isomerizes to L-ascorbate. While most higher animals can synthesize asborbic acid, primates and guinea pigs cannot [3].
History
EC 1.1.3.8 created 1965, modified 2001, modified 2006
Pathway
ec00053  Ascorbate and aldarate metabolism
ec01100  Metabolic pathways
Orthology
K00103  L-gulonolactone oxidase
Genes
MMUR105877128
OGA100956712
MMU268756(Gulo)
MCAL110308887
MPAH110325590
RNO60671(Gulo)
MCOC116084654
MUN110559217
CGE100760306
PLEU114707320
NGI103730028
HGL101717756
CPOC100735706
CCAN109692534
DORD105991536
DSP122123508
NCAR124983878
OCU100341843
OPI101534528
TUP102480281
CFA486100
VVP112926784
VLG121498164
AML100478547
UMR103675989
UAH113270039
UAR123777422
ELK111155991
LLV125092970
MPUF101690348
ORO101374448
EJU114217386
ZCA113924112 113929666
MLX118009683 118022113
FCA101092602
PYU121022590
PBG122473886
PTG102964688
PPAD109262342
AJU106973111
HHV120220294
BTA286812(GULO)
BOM102268085
BIU109562556
BBUB102410395
CHX102186117
OAS101106395
ODA120860354 120866108
CCAD122432065
SSC396759(GULO)
CFR102517517
CBAI105063867
CDK105102219
VPC102535238
BACU103015173
LVE103082082
OOR101282721
DLE111185404
PCAD102976415
PSIU116755532
ECB100060483
EPZ103550421
EAI106832515
HAI109379502(GULO)
RFQ117038035
PALE102892731
PGIG120613175
RAY107510766
MJV108394330
TOD119258270
SARA101545335
LAV100658302
TMU101343971
DNM101447362
MDO100030569
GAS123235009
SHR100931979
PCW110206533
OAA100093190
GGA770996
PCOC116235640
MGP100548691
CJO107312420
NMEL110396328
APLA101802453
ACYG106049515
AFUL116487527
TGU115494645
LSR110477408
PMOA120510390
OTC121341613
FAB101806211
PHI102114090
CCAE111927479
ETL114072263 114072641 114073445
FPG101922774
FCH102048039
CLV102086252
EGZ104132031
NNI104014769
ACUN113478833
TALA104357285
PADL103917790
ACHC115351120
AAM106486384
AROW112966665
NPD112948905
DNE112993190
ASN102383993
AMJ102559022
CPOO109311561
GGN109300224
PSS102452718
CPIC101948999
TST117871397
CABI116837372
MRV120401859
ACS100564818
PVT110089492
SUND121921175
PBI103058013
PMUR107295744
TSR106552982
PGUT117667687
VKO123033611
PMUA114594945
ZVI118082815
GJA107110087
XLA495407
XTR101733882
NPR108796920
RTEM120936049
BBUF120997150
BGAR122934154
LOC102697922
PSPA121317358
ARUT117410447
CMK103191462
RTP109916960
BFO118408636 118410004 118412674
BBEL109466823 109478142 109485933
CIN100180753
SPU100888205 115917982 764246
APLC110982436 110990091
SKO100368812
LCQ111689991
CSEC111874443
FCD110843230 110844959 110852090 110857910 110860799
EAF111704487
ISCIscW_ISCW015963
DSV119433475
RSAN119404685
RMP119181904
DPTE113788500
DFR124496172
CSCU111616823 111631909
PTEP107443895
SDM118196990
LGILOTGIDRAFT_208933
PCAN112559251
BGT106064532
GAE121381575
HRF124138198 124146837
HRJ124268692 124278077
LAK106161198
NVE5496930 5516954
EPA110254170
ATEN116306443
ADF107336978 107354754
AMIL114949995 114975807
PDAM113666867 113684795
SPIS111331459 111343392
DGT114518388
XEN124452715
TADTRIADDRAFT_18858
AQU100633693
ATHAT1G32300(GulLO1) AT2G46740(GulLO5) AT2G46750(GulLO2) AT2G46760(GulLO6) AT5G11540(GulLO3) AT5G56470(GulLO7) AT5G56490(GulLO4)
ALY9300533 9307550 9316330 9318153 9318154 9321645 9327042
CRB111831458 17876581 17881389 17888997 17889214 17898641
CSAT104705420 104726195 104733303 104733318 104733335 104735167 104763518 104763519 104769386 104773365 104773373 104784633 104784635 104788736 104793653
EUSEUTSA_v10001364mg EUTSA_v10001763mg EUTSA_v10002773mg EUTSA_v10002834mg EUTSA_v10013068mg
BRP103845085 103850775 103851822 103866436 103866437 103866439
BNA106370317 106383023 106383183 106383563 106412350 106414144 106415381 106418293 106441738 106450477 106450478 106454421
BOE106318605 106321214 106322885 106323392 106337761 106339293 106340076
RSZ108806751 108807290 108810407 108824642 108826635 108836004 108837749 108842920
THJ104814776 104817396 104824215
CPAP110808475 110817648
CIT102613738 102618839 102619065
CICCICLE_v10007892mg CICLE_v10019421mg CICLE_v10031018mg
PVY116109016 116114638
MINC123194448 123216243 123222778
TCC18587950 18613903
GRA105763150 105763957
GHI107919393 107923325 107953839
GAB108472389 108478560
DZI111284673 111305985 111313977
EGR104421946 104437765 104442399
GMX100778616 100787286 100789425 100790483 100791439 100799814 100801515 100806810
GSJ114388930 114389546 114390298 114394243 114398327 114399603 114407834 114418271
PVUPHAVU_001G074300g PHAVU_009G257400g PHAVU_009G257600g PHAVU_010G118400g
VRA106760021 106760022 106760023 106764193 106780334
VAR108322237 108322273 108336890 108346983
VUN114164180 114164578 114166177 114193442
CCAJ109797626 109802335 109806109
APRC113847457 113851070 113862093 113863005
MTRMTR_1g026680 MTR_1g026710 MTR_5g005540 MTR_8g024560
CAM101495962 101505780 101507144
LJALj1g3v5060530.1(Lj1g3v5060530.1) Lj1g3v5060540.1(Lj1g3v5060540.1) Lj2g3v3250000.1(Lj2g3v3250000.1) Lj3g3v0462020.1(Lj3g3v0462020.1) Lj3g3v0462290.1(Lj3g3v0462290.1) Lj3g3v2483050.1(Lj3g3v2483050.1)
ADU107472904 107491831 107495150 107495223
AIP107605189 107605190 107624247 107648068
AHF112695261 112726583 112734285 112754172 112755425 112755430 112755578 112755579
LANG109337324 109337326 109344618 109351734 109357115
FVE101306399 101309401 101309682
RCN112172675 112181889 112195429 112196598
PPER18785965 18791334
PMUM103320196 103332140
PAVI110757668 110761276
PDUL117614009 117619201
MDM103415463 103420706 103434065 103441169
PXB103937841 103956368 103957464
ZJU107403832 107403833 107403834 107404356 107420638 107423797 107423798 107423856 107423857 107423858
MNT21390658 21406194
CSV101221035
CMO103483108
BHJ120073810
MCHA111013948
CMAX111474382
CMOS111448536
CPEP111800661
RCU8266308 8271194 8284093
JCU105643326
HBR110661050 110667924 110672771
MESC110610798 110615155
POP112323644 7458969 7487736 7487737
PEU105126260 105126261 105127151
PALZ118051029 118063447 118063448
JRE108979173 109007376
QSU112008333 112008334 112025667
QLO115954459 115954461 115956976 115969929
TWL119996183 120003532 120003765
VVI100247576 100260872
VRI117912408 117931768
SLY101256030 101261840
SPEN107027329 107029833
SOT102582456 102585226 102586137 102587810 102603855
CANN107843037 107879723
NTA107764580 107768458 107777550 107784038 107803386 107811679 107824953
NSY104213025 104234660 104245144
NTO104091950 104093860 104107894
NAU109213786 109214899 109229242
INI109175297 109175341 109178643 109178731 109178732
ITR116001767 116001970 116001971 116006391 116012272
SIND105156090 105164756 105178927
OEU111379529 111391130
EGT105963955 105967143
SSPL121767404 121778219 121780982 121782090
HAN110874959 110879630 110879631 110883880 110900985
ECAD122598455 122610424
LSV111879985 111885866
CCAV112523679
DCR108208095 108209257 108225989
CSIN114266476 114268684 114276226 114321799
BVG104885147 104892369 104892687 104903879 104903969 104907984
SOE110775241 110778450 110778451 110782212 110784304 110784305
CQI110705857 110711421 110714229 110714524 110722103 110722104 110731645 110731774
NNU104593673 104595141 104604665
MING122073468 122076863 122093392 122093453
TSS122655975 122656118 122666101
PSOM113298738 113313591 113327844 113340946 113342608 113348225 113348226 113349135 113352362 113357463 113361107
NCOL116260054 116263615 116263966 116263991 116263996 116264555 116264612 116267246
OSA4327936 4328142 4328143 4333257 4344509 4346054
DOSAOs01t0687800-00(Os01g0687800) Os02t0124400-01(Os02g0124400) Os02t0124600-00(Os02g0124600) Os03t0563100-01(Os03g0563100) Os04t0360500-01(Os04g0360500) Os04t0361500-00(Os04g0361500) Os08t0114300-01(Os08g0114300) Os08t0519100-01(Os08g0519100)
OBR102704284 102714071 102715368 102717331 102718199
BDI100824967 100831264 100833703 100839560 100840029 100846108 104583572
ATS109741952 109742231 109742755 109745928 109746445 109759156 109762936 109762941 109765633 109765918 109770978 109771109 109772774 109774088 109774089 109783075 109784390 109785934 109787576
TDC119282497 119292133 119292134 119294227 119294330 119294351 119294357 119298602 119300514 119300517 119301285 119302670 119304219 119311350 119311351 119314791 119316267 119321288 119326322 119328328 119331420 119331856 119331883 119335486 119336168 119336282 119343115 119344251 119352850 119357442
TAES123048285 123066746 123089611 123089612 123089614 123089822 123089862 123092822 123093851 123094025 123097240 123099264 123100631 123100633 123100765 123101824 123101825 123101827 123101829 123106721 123107558 123108491 123116691 123116692 123119844 123125279 123131962 123132483 123135990 123136553 123141964 123143483 123144060 123144985 123147570 123150957 123152785 123155524 123157605 123160313 123160726 123163995 123164389 123165451 123168064 123169611 123169788 123169797 123177331 123179789 123184342
SBI8065667 8068565 8069704 8081626
ZMA100286152 100501771 103640501 103653136
SITA101756668 101758891 101760109 101767367 101776642 101777017
SVS117834550 117834554 117836368 117843721 117860010 117861505
PVIR120648401 120655450 120667685 120678572 120678668 120679522 120693536 120698286 120707520 120711194 120712184 120712304
PHAI112873947 112895835 112896340 112896444 112896569 112902233
PDA103696384 103706838 103707507
EGU105042184 105047414 105055864 105059298
MUS103976003 103976004 103976375 103976376 103976377 103981555 103981650 108952122
DCT110098544 110113778
PEQ110018048 110033379
AOF109819924 109819925 109828244 109835304 109837064 109848476
ATR18426700 18430107 18436500
SMOSELMODRAFT_100820 SELMODRAFT_115895 SELMODRAFT_115915 SELMODRAFT_115966 SELMODRAFT_115992 SELMODRAFT_123507 SELMODRAFT_130502 SELMODRAFT_134470 SELMODRAFT_134896 SELMODRAFT_137111 SELMODRAFT_158414 SELMODRAFT_171869 SELMODRAFT_181802 SELMODRAFT_235822 SELMODRAFT_236475 SELMODRAFT_403566 SELMODRAFT_403567 SELMODRAFT_411795 SELMODRAFT_411796 SELMODRAFT_429865 SELMODRAFT_430460 SELMODRAFT_76913 SELMODRAFT_77373 SELMODRAFT_91192 SELMODRAFT_94673 SELMODRAFT_94694 SELMODRAFT_97935
PPP112274181 112280369 112283902 112284099 112285475
MNGMNEG_3620 MNEG_4091 MNEG_4092 MNEG_5061 MNEG_8588
GSLGasu_21710
PANPODANSg2036
MTMMYCTH_44863
MGRMGG_02420
TMNUCRPA7_4838
FGRFGSG_12106
FPUFPSE_00272
FVRFVEG_13531
FOXFOXG_07143 FOXG_14711
NHENECHADRAFT_89432
CMTCCM_00852
VALVDBG_01857
VDAVDAG_01698
CFJCFIO01_02936
ELAUCREL1_857
PFYPFICI_03033
MBEMBM_05135
BGHBDBG_07089
BSCCOCSADRAFT_156375
FFUCLAFUR5_08886 CLAFUR5_09113
NPAUCRNP2_4485
CNECNN01640
CNBCNBN1590
CNGCNAG_06439
CGICGB_N2380W
TMSTREMEDRAFT_45215
TASAA1Q1_02359
TVSTRAVEDRAFT_30092
DSQDICSQDRAFT_155883
PCOPHACADRAFT_261187
SHSSTEHIDRAFT_123099
HIRHETIRDRAFT_174060 HETIRDRAFT_63784
PSQPUNSTDRAFT_106850 PUNSTDRAFT_110662
ADLAURDEDRAFT_138376 AURDEDRAFT_79130
FMEFOMMEDRAFT_80869
GTRGLOTRDRAFT_121214 GLOTRDRAFT_78381
LBCLACBIDRAFT_248838 LACBIDRAFT_254417
MRRMoror_5342 Moror_765
MOREE1B28_013525
CCICC1G_11199
SCMSCHCODRAFT_237513 SCHCODRAFT_60077
ABPAGABI1DRAFT115961(AGABI1DRAFT_115961)
ABVAGABI2DRAFT195196(AGABI2DRAFT_195196)
CPUTCONPUDRAFT_139415
SLASERLADRAFT_366246
WSEWALSEDRAFT_16718
WICJ056_004700
UMAUMAG_10013
PFPPFL1_00180
MGLMGL_0958
MRTMRET_3192
MSYMMSY001_2664
PGRPGTG_07192
MLRMELLADRAFT_25325
ACANACA1_367390
GTTGUITHDRAFT_122678
TBRTb927.5.2650
TBGTbgDal_V3730
TCR507047.150
LMALMJF_17_1360
LIFLINJ_17_1470
LDOLDBPK_171470
LMILMXM_17_1360
LBZLBRM_17_1490
LPANLPMP_171320
NGRNAEGRDRAFT_62943
VCYIX92_07100
VCTJV59_31390
VAQFIV01_07260(xyoA)
MIIBTJ40_09190
MICTFIU95_07405
MICZGL2_31180
HCHHCH_04675
HAHEENC22_09430
BSANCHH28_09040
LITFPZ52_18145
CYTcce_4306
RIVRiv7116_6302
SFCSpiaf_0243
KDIKrodi_2009
DODDCS32_01750
PRNBW723_08345
PHALH9I45_13120
WFUAXE80_11085
 » show all
Reference
1  [PMID:14405898]
  Authors
ISHERWOOD FA, MAPSON LW, CHEN YT.
  Title
Synthesis of L-ascorbic acid in rat-liver homogenates. Conversion of L-gulono- and L-galactono-gamma-lactone and the respective acids into L-ascorbic acid.
  Journal
Biochem J 76:157-71 (1960)
DOI:10.1042/bj0760157
Reference
2  [PMID:7138847]
  Authors
Kiuchi K, Nishikimi M, Yagi K.
  Title
Purification and characterization of L-gulonolactone oxidase from chicken kidney microsomes.
  Journal
Biochemistry 21:5076-82 (1982)
DOI:10.1021/bi00263a035
Reference
3  [PMID:8175804]
  Authors
Nishikimi M, Fukuyama R, Minoshima S, Shimizu N, Yagi K.
  Title
Cloning and chromosomal mapping of the human nonfunctional gene for L-gulono-gamma-lactone oxidase, the enzyme for L-ascorbic acid biosynthesis missing in man.
  Journal
J Biol Chem 269:13685-8 (1994)
Reference
4
  Authors
Chatterjee, I.B., Chatterjee, G.C., Ghosh, N.C. and Guha, B.C.
  Title
Identification of 2-keto-L-gulonolactone as an intermediate in the biosynthesis of L-ascorbic acid.
  Journal
Naturwissenschaften 46:475 (1959)
Other DBs
ExplorEnz - The Enzyme Database: 1.1.3.8
IUBMB Enzyme Nomenclature: 1.1.3.8
ExPASy - ENZYME nomenclature database: 1.1.3.8
BRENDA, the Enzyme Database: 1.1.3.8
CAS: 9028-78-8

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