KEGG   ENZYME: 1.1.9.1
Entry
EC 1.1.9.1                  Enzyme                                 
Name
alcohol dehydrogenase (azurin);
type II quinoprotein alcohol dehydrogenase;
quinohaemoprotein ethanol dehydrogenase;
QHEDH;
ADHIIB
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With a copper protein as acceptor
Sysname
alcohol:azurin oxidoreductase
Reaction(IUBMB)
a primary alcohol + azurin = an aldehyde + reduced azurin [RN:R09480]
Reaction(KEGG)
R09480
Substrate
primary alcohol [CPD:C00226];
azurin
Product
aldehyde [CPD:C00071];
reduced azurin [CPD:C05358]
Comment
A soluble, periplasmic PQQ-containing quinohemoprotein. Also contains a single heme c. Occurs in Comamonas and Pseudomonas. Does not require an amine activator. Oxidizes a wide range of primary and secondary alcohols, and also aldehydes and large substrates such as sterols; methanol is not a substrate. Usually assayed with phenazine methosulfate or ferricyanide. Like all other quinoprotein alcohol dehydrogenases it has an 8-bladed 'propeller' structure, a calcium ion bound to the PQQ in the active site and an unusual disulfide ring structure in close proximity to the PQQ.
History
EC 1.1.9.1 created 2010 as EC 1.1.98.1; transferred 2011 to EC 1.1.9.1
Orthology
K17760  quinohemoprotein ethanol dehydrogenase
Genes
PMKMDS_2680
PCQPcP3B5_33080(qgdA_1)
PMUIG4G71_16070
PPWPputW619_2696
PSZPSTAB_2905
PSRPSTAA_3020
PSHPsest_1423
PSTUUIB01_10105
PSTTCH92_09640
PCHLLLJ08_06255 LLJ08_09455
PBMCL52_14245
PSKU771_15535
PPSYAOC04_06000
PSEDDM292_04320 DM292_07550
PUMHGP31_10690
POJPtoMrB4_39170
PXAKSS93_18230
PQIKH389_15205 KH389_15915 KH389_17020
PSHHHU773_015410
PNNKEM63_08795
PASGKSS96_11385
PFAKKSS94_13150
PALVKSS97_17250
PTWTUM18999_41850
PTAENCTC10697_01924(qheDH)
PCAVD3880_11910
PBBAKN87_00160
HPEGEAO82_13780
HAESLO767_05585
MSRAU15_01600
MARIACP86_11840
MLQASQ50_13195
MARJMARI_09920(qheDH)
SALKFBQ74_01340
GPSC427_3776
SPOIIMCC21906_02691
SNANI6N98_04765 I6N98_07195 I6N98_13860
ZALAZF00_00410 AZF00_02915 AZF00_06825
OSGBST96_06520 BST96_11945 BST96_14245 BST96_17020
HJABST95_15490
HALCEY643_01600
KIMG3T16_00650 G3T16_16955
AFUSEYZ66_01320 EYZ66_01385 EYZ66_03410 EYZ66_03590 EYZ66_06330
ADIB5T_04379(exaA1)
AXEP40_21330
SDFACG33_03485
SOKD0B54_04380 D0B54_04385
FCEJN531_004925 JN531_005045
PSPIPS2015_1348 PS2015_2383
REHH16_A1884(h16_A1884)
CNCCNE_1c18660(qheDH) CNE_BB1p04310(adh)
CUHBJN34_09985
REUReut_A1461 Reut_B4160
CTIRALTA_A1577(exaA1)
CBWRR42_s2370
CCUPBKK81_18975 BKK81_27650
CUPBKK80_22965 BKK80_28675
CUUBKK79_28240 BKK79_34625
COXE0W60_10280
AMIMMIM_c00090(qheDH1)
POLBpro_5302
VEIVeis_0427
DACDaci_3352
DELDelCs14_3417
DTSBI380_02445
DHKBO996_15355
DLAI6G47_06900 I6G47_09325
VAPVapar_5018
VPEVarpa_5729
VPDVAPA_1c52090
VBOCKY39_31100
VAMC4F17_20615
CTTCtCNB1_0197 CtCNB1_1388
CTESO987_00960
OTKC6570_08100
HYBQ5W_16030
HPSEHPF_13145(qheDH)
HYNF9K07_23870
PBHAAW51_3568(qheDH)
MPTMpe_A0877 Mpe_A0905
METPC1M51_13570 C1M51_13725
ATERMW290_13615 MW290_24600
DOEDENOEST_0490(qbdA) DENOEST_2039(qbdA) DENOEST_3548(qbdA) DENOEST_3675(qbdA)
MBACBN1209_0896
MEUACJ67_07920
AZOazo3022(exaA4)
AOAdqs_3157
AZRCJ010_18755 CJ010_22255
THUAC731_008560
TAKTharo_3323
AGEAA314_07777
NIYFQ775_00490
PLAPlav_1306
PMOBHG718_13715
RBSRHODOSMS8_03066(qbdA)
BJUBJ6T_73570
BBTBBta_5502
BICLMTR13_35850
BRKCWS35_13515
BSYMCIT39_22055
BVZBRAD3257_1848(qbdA)
RPARPA3188
RPBRPB_2355
RPDRPD_3110
RPERPE_3525
RPTRpal_3601
RPXRpdx1_2242
RBKE0H22_16105
VGOGJW-30_1_04338(qheDH)
TRBHB776_05770
XDIEZH22_02215
HMCHYPMC_4861(adh)
NTDEGO55_06120 EGO55_07055
PSINCAK95_13895
RBMTEF_09160
LABPFJ695_24050
CMBCSW64_14395
CAULKCG34_00855 KCG34_05195
PZUPHZ_c3112
PHBHYN04_10065
TSVDSM104635_00613(qgdA)
CIDP73_2982
THAACFI11_17745
THASC6Y53_11405
HNEHNE_0129(adhA)
HBCAEM38_01780
NARSaro_2870 Saro_2878
NPPPP1Y_AT11408 PP1Y_AT11547 PP1Y_Mpl5286
NPNJI59_21485
NREBES08_11360 BES08_19965 BES08_22630 BES08_27330
NOVTQ38_020540 TQ38_024820
NOTC7W88_07620 C7W88_07660
NORFA702_09260 FA702_10240 FA702_20765
NGFFRF71_08640
NDRHT578_07545 HT578_07615 HT578_09220 HT578_14485
SALSala_0379
SPHKSKP52_17900(adh)
SPHPLH20_16360
SMAGAN936_03210 AN936_16350
SMAZLH19_18005 LH19_18030 LH19_18835
STERAOA14_02595
SGISGRAN_3454(adhA2)
SPHQBWQ93_13035
SPHOC3E99_12080
SPHXE5675_02990
SPHUSPPYR_0186(qbdA)
SWISwit_0721 Swit_1001 Swit_2227 Swit_5136
SPHDHY78_14390 HY78_21510 HY78_23090
RDICMV14_16905 CMV14_19410
SPHITS85_07850
SPKCKC8_09425
SPHCCVN68_17415
SLANGV829_11830
SSUAFPZ54_18055
SJPSJA_C1-02010 SJA_C1-02070 SJA_C2-04000
SINBSIDU_17200 SIDU_17230
SCHSphch_0592 Sphch_0603 Sphch_2127
SSYSLG_05620 SLG_07280 SLG_12260 SLG_12290 SLG_18610 SLG_20920
SYBTZ53_16615 TZ53_17030 TZ53_17090
SPHBEP837_00323 EP837_00335(exaA) EP837_03710
SPHRBSY17_3920(qbdA) BSY17_934(qbdA)
SPHTK426_12160 K426_12220
SHYDCJD35_16290 CJD35_16630 CJD35_20620
SYAA6768_05740 A6768_05805 A6768_20115
SCLOSCLO_1006180 SCLO_1006300
SPYGYGS_C2P0050 YGS_C2P0418
SUFLFIL70_03770 FIL70_05365 FIL70_05425
SBARH5V43_00800 H5V43_04645 H5V43_05050 H5V43_05110
SAMISAMIE_1016260 SAMIE_1019310
SPPHKFK14_04690 KFK14_08020 KFK14_10340 KFK14_13445 KFK14_17040
SPHGAZE99_03780 AZE99_04575 AZE99_04655
SFLASPHFLASMR4Y_01514(qbdA)
SPHYCHN51_01650
SPHLLPB140_10885
BLASBSY18_3165(adhA) BSY18_3429(qgdA)
BFWB5J99_06725 B5J99_07940
SPHJBSL82_14255 BSL82_14300
SPZRG5C33_07505
PALGHFP57_17830
PARJJ4G78_09460
PHAOHF685_12515
SMICSmB9_16520 SmB9_26030(exaA) SmB9_32770
AEPAMC99_00019
ALBAEB_P1071 AEB_P2933 AEB_P2938
ALHG6N82_06175 G6N82_10280
AAYWYH_01144(qbdA_1) WYH_01921(qbdA_3) WYH_02888(qbdA_4) WYH_03122(qgdA) WYH_03187(qbdA_5)
ADOA6F68_00780(qbdA)
EFVCHH26_02510
ELIELI_14570
ELQGa0102493_111321
ERKCD351_00730
ERRDVR09_00745
ERFFIU90_00555(qbdA1) FIU90_08125(qbdA2)
EMVHQR01_13325
PNSA9D12_03105
PORLBG023_11102
PHZCHX26_00475
POTE2E27_01630
ECOGFIV45_03450 FIV45_11680
PARHI5S86_14975
OCNCUC15_15200
LYGC1N55_08345
VIMGWK91_01760
PSYHD0S48_16125
PSYOPB01_16660
NMKCHR53_27745
PAEKD3873_10820
MYLC3E77_05855
MUZH4N58_01985
AMSAMIS_77470
AIHAiant_38910
CWOCwoe_1286
TALBFTW19_18675
EDAGWR55_07775
ABACLuPra_01740(qgdA_1) LuPra_02092(qbdA_3)
CHKD4L85_24450
ASAGFGM00_10090
 » show all
Reference
1  [PMID:3521592]
  Authors
Groen BW, van Kleef MA, Duine JA
  Title
Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni.
  Journal
Biochem J 234:611-5 (1986)
DOI:10.1042/bj2340611
Reference
2  [PMID:7626615]
  Authors
de Jong GA, Caldeira J, Sun J, Jongejan JA, de Vries S, Loehr TM, Moura I, Moura JJ, Duine JA
  Title
Characterization of the interaction between PQQ and heme c in the quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni.
  Journal
Biochemistry 34:9451-8 (1995)
DOI:10.1021/bi00029a021
Reference
3  [PMID:7730276]
  Authors
Toyama H, Fujii A, Matsushita K, Shinagawa E, Ameyama M, Adachi O
  Title
Three distinct quinoprotein alcohol dehydrogenases are expressed when Pseudomonas putida is grown on different alcohols.
  Journal
J Bacteriol 177:2442-50 (1995)
DOI:10.1128/JB.177.9.2442-2450.1995
Reference
4  [PMID:10320337]
  Authors
Matsushita K, Yamashita T, Aoki N, Toyama H, Adachi O
  Title
Electron transfer from quinohemoprotein alcohol dehydrogenase to blue copper protein azurin in the alcohol oxidase respiratory chain of Pseudomonas putida HK5.
  Journal
Biochemistry 38:6111-8 (1999)
DOI:10.1021/bi990121f
Reference
5  [PMID:12057198]
  Authors
Chen ZW, Matsushita K, Yamashita T, Fujii TA, Toyama H, Adachi O, Bellamy HD, Mathews FS
  Title
Structure at 1.9 A resolution of a quinohemoprotein alcohol dehydrogenase from Pseudomonas putida HK5.
  Journal
Structure 10:837-49 (2002)
DOI:10.1016/S0969-2126(02)00774-8
  Sequence
Reference
6  [PMID:11714714]
  Authors
Oubrie A, Rozeboom HJ, Kalk KH, Huizinga EG, Dijkstra BW
  Title
Crystal structure of quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni: structural basis for substrate oxidation and electron transfer.
  Journal
J Biol Chem 277:3727-32 (2002)
DOI:10.1074/jbc.M109403200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.9.1
IUBMB Enzyme Nomenclature: 1.1.9.1
ExPASy - ENZYME nomenclature database: 1.1.9.1
BRENDA, the Enzyme Database: 1.1.9.1

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