KEGG   ENZYME: 1.1.99.37
Entry
EC 1.1.99.37                Enzyme                                 
Name
methanol dehydrogenase (nicotinoprotein);
NDMA-dependent methanol dehydrogenase;
nicotinoprotein methanol dehydrogenase;
methanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With unknown physiological acceptors
Sysname
methanol:acceptor oxidoreductase
Reaction(IUBMB)
methanol + acceptor = formaldehyde + reduced acceptor [RN:R09553]
Reaction(KEGG)
R09553
Substrate
methanol [CPD:C00132];
acceptor [CPD:C00028]
Product
formaldehyde [CPD:C00067];
reduced acceptor [CPD:C00030]
Comment
Contains Zn2+ and Mg2+. Nicotinoprotein methanol dehydrogenases have a tightly bound NADP+/NADPH cofactor that does not dissociate during the catalytic process. Instead, the cofactor is regenerated by a second substrate or electron carrier. While the in vivo electron acceptor is not known, N,N-dimethyl-4-nitrosoaniline (NDMA), which is reduced to 4-(hydroxylamino)-N,N-dimethylaniline, can serve this function in vitro. The enzyme has been detected in several Gram-positive methylotrophic bacteria, including Amycolatopsis methanolica, Rhodococcus rhodochrous and Rhodococcus erythropolis [1-3]. These enzymes are decameric, and possess a 5-fold symmetry [4]. Some of the enzymes can also dismutate formaldehyde to methanol and formate [5].
History
EC 1.1.99.37 created 2010
Orthology
K17067  formaldehyde dismutase / methanol dehydrogenase
Genes
GEBGM18_3226
GPIGPICK_06680
GURGura_3568
DEXHWD60_01710(mdo)
DVNHQ394_08800(mdo)
DAEDtox_4270
MGOAFA91_03425
MDXBTO20_03135 BTO20_38200
MSMMSMEG_6242
MSGMSMEI_6081
MSBLJ00_30865
MSNLI99_30870
MSHLI98_30875
MVAMvan_5479
MCBMycch_5913
MNED174_25530
MYNMyAD_25070
MLLB1R94_22390
MRHMycrhN_3030
MAUUNCTC10437_05223(thcE)
MTYMTOK_19990
MPSCMPSYJ_14360
MANYMANY_50600
MPOFMPOR_01100
MMATMMAGJ_31270
ASDAS9A_4308
NFANFA_22240
NFRERS450000_01873(thcE)
RHARHA1_ro06057
RERRER_17770
REYO5Y_08520
REBXU06_08765
RQIC1M55_08995(mdo)
ROPROP_61160
ROAPd630_LPD02697
RPYY013_08845
RBYCEJ39_19735(mdo)
RHBNY08_1972
RAVAAT18_18670
RFAA3L23_00307
RHSA3Q41_03101
RRZCS378_08140(mdo)
RHUA3Q40_00401
RHQIM25_00685
RHODAOT96_19365
RRT4535765_03582(thcE)
RKOJWS14_33040(mdo)
RGOKYT97_09645(mdo)
GBRGbro_1931
GPOGPOL_c18220 GPOL_c38190
GORKTR9_1872
GOQACH46_07270
GTABCM27_10050
GOCCXX93_15365(mdo)
GITC6V83_07720(mdo)
GRUGCWB2_09440(thcE)
GOMD7316_01326(mno) D7316_03687(thcE)
GAVC5O27_18320(mdo)
GODGKZ92_09040(mdo)
GAMGII34_08675(mdo)
GPDGII33_01450(mdo) GII33_08060(mdo)
GJIH1R19_09330(mdo)
GOILK459_01040(mdo) LK459_01660(mdo)
TSMASU32_17935
DTMBJL86_1140
DIZCT688_01100(mdo)
DPCA6048_01130
TOYFO059_05730(mdo)
SHYSHJG_2446
SHOSHJGH_2211
STUIGCM10017668_53940
ALXLVQ62_05600(mdo)
PHWG7075_01320(mdo)
NAROCFH99_06345(mdo)
NAQUENKNEFLB_00974(mno)
NROK8W59_04780(mdo)
NMLNamu_0777
SENSACE_2386
SACGFDZ84_34730(mdo)
AMQAMETH_5577(mdo)
PSEAWY02_13685
PSEEFRP1_00395 FRP1_00630
PSEHXF36_28100
PSEQAD006_08035 AD006_08270
PECQAD017_15870 AD017_16100
PHHAFB00_08750 AFB00_13725
PAUTPdca_61440 Pdca_62050
PBROHOP40_10780(mdo)
RRDRradSPS_2629
RUBGBA63_20895
RMARGBA65_20385(mdo)
 » show all
Reference
1  [PMID:1995642]
  Authors
Vonck J, Arfman N, De Vries GE, Van Beeumen J, Van Bruggen EF, Dijkhuizen L
  Title
Electron microscopic analysis and biochemical characterization of a novel methanol dehydrogenase from the thermotolerant Bacillus sp. C1.
  Journal
J Biol Chem 266:3949-54 (1991)
Reference
2  [PMID:8385013]
  Authors
Van Ophem PW, Van Beeumen J, Duine JA
  Title
Nicotinoprotein [NAD(P)-containing] alcohol/aldehyde oxidoreductases. Purification and characterization of a novel type from Amycolatopsis methanolica.
  Journal
Eur J Biochem 212:819-26 (1993)
DOI:10.1111/j.1432-1033.1993.tb17723.x
Reference
3  [PMID:8449887]
  Authors
Bystrykh LV, Vonck J, van Bruggen EF, van Beeumen J, Samyn B, Govorukhina NI, Arfman N, Duine JA, Dijkhuizen L
  Title
Electron microscopic analysis and structural characterization of novel NADP(H)-containing methanol: N,N'-dimethyl-4-nitrosoaniline oxidoreductases from the gram-positive methylotrophic bacteria Amycolatopsis methanolica and Mycobacterium gastri MB19.
  Journal
J Bacteriol 175:1814-22 (1993)
DOI:10.1128/JB.175.6.1814-1822.1993
Reference
4  [PMID:12351635]
  Authors
Hektor HJ, Kloosterman H, Dijkhuizen L
  Title
Identification of a magnesium-dependent NAD(P)(H)-binding domain in the nicotinoprotein methanol dehydrogenase from Bacillus methanolicus.
  Journal
J Biol Chem 277:46966-73 (2002)
DOI:10.1074/jbc.M207547200
Reference
5  [PMID:19875438]
  Authors
Park H, Lee H, Ro YT, Kim YM
  Title
Identification and functional characterization of a gene for the methanol : N,N'-dimethyl-4-nitrosoaniline oxidoreductase from Mycobacterium sp. strain JC1 (DSM 3803).
  Journal
Microbiology 156:463-71 (2010)
DOI:10.1099/mic.0.034124-0
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.99.37
IUBMB Enzyme Nomenclature: 1.1.99.37
ExPASy - ENZYME nomenclature database: 1.1.99.37
BRENDA, the Enzyme Database: 1.1.99.37

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