KEGG   ENZYME: 1.1.99.41
Entry
EC 1.1.99.41                Enzyme                                 
Name
3-hydroxy-1,2-didehydro-2,3-dihydrotabersonine reductase;
T3R;
tabersonine 3-reductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With unknown physiological acceptors
Sysname
(3R)-3-hydroxy-16-methoxy-2,3-dihydrotabersonine:acceptor oxidoreductase
Reaction(IUBMB)
(1) (3R)-3-hydroxy-16-methoxy-2,3-dihydrotabersonine + acceptor = (3R)-3-hydroxy-16-methoxy-1,2-didehydro-2,3-dihydrotabersonine + reduced acceptor [RN:R11777];
(2) (3R)-3-hydroxy-2,3-dihydrotabersonine + acceptor = (3R)-3-hydroxy-1,2-didehydro-2,3-dihydrotabersonine + reduced acceptor [RN:R11802]
Reaction(KEGG)
R11777 R11802
Substrate
(3R)-3-hydroxy-16-methoxy-2,3-dihydrotabersonine [CPD:C04578];
acceptor [CPD:C00028];
(3R)-3-hydroxy-2,3-dihydrotabersonine [CPD:C21682]
Product
(3R)-3-hydroxy-16-methoxy-1,2-didehydro-2,3-dihydrotabersonine [CPD:C21678];
reduced acceptor [CPD:C00030];
(3R)-3-hydroxy-1,2-didehydro-2,3-dihydrotabersonine [CPD:C21683]
Comment
This enzyme is involved in the biosynthesis of vindoline and vindorosine in the plant Catharanthus roseus (Madagascar periwinkle). In vivo, it functions in the direction of reduction. It has no activity with 3-epoxylated compounds, which can form spontaneously from its unstable substrates.
History
EC 1.1.99.41 created 2017
Pathway
ec00901  Indole alkaloid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K22323  3-hydroxy-1,2-didehydro-2,3-dihydrotabersonine reductase
Reference
1  [PMID:25918424]
  Authors
Qu Y, Easson ML, Froese J, Simionescu R, Hudlicky T, De Luca V
  Title
Completion of the seven-step pathway from tabersonine to the anticancer drug precursor vindoline and its assembly in yeast.
  Journal
Proc Natl Acad Sci U S A 112:6224-9 (2015)
DOI:10.1073/pnas.1501821112
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.99.41
IUBMB Enzyme Nomenclature: 1.1.99.41
ExPASy - ENZYME nomenclature database: 1.1.99.41
BRENDA, the Enzyme Database: 1.1.99.41

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