KEGG   ENZYME: 1.10.3.15Help
Entry
EC 1.10.3.15                Enzyme                                 

Name
grixazone synthase;
GriF
Class
Oxidoreductases;
Acting on diphenols and related substances as donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
3-amino-4-hydroxybenzoate:N-acetyl-L-cysteine:oxygen oxidoreductase
Reaction(IUBMB)
2 3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + 2 O2 = grixazone B + 4 H2O + CO2 [RN:R10720]
Reaction(KEGG)
Substrate
3-amino-4-hydroxybenzoate [CPD:C12115];
N-acetyl-L-cysteine [CPD:C06809];
O2 [CPD:C00007]
Product
grixazone B [CPD:C20799];
H2O [CPD:C00001];
CO2 [CPD:C00011]
Comment
A type 3 multi copper protein. The enzyme, isolated from the bacterium Streptomyces griseus, catalyses an 8 electron oxidation. Activation of the enzyme requires a copper chaperone (GriE). It also acts on 3-amino-4-hydroxybenzaldehyde, giving grixazone A. The second aldehyde group is presumably lost as formate. The enzyme also catalyses the reaction of EC 1.10.3.4 o-aminophenol oxidase.
History
EC 1.10.3.15 created 2014
Orthology
K20204  grixazone synthase / o-aminophenol oxidase
Genes
SGR: SGR_4246(griF)
Taxonomy
Reference
1  [PMID:17003031]
  Authors
Suzuki H, Ohnishi Y, Furusho Y, Sakuda S, Horinouchi S.
  Title
Novel benzene ring biosynthesis from C(3) and C(4) primary metabolites by two enzymes.
  Journal
J Biol Chem 281:36944-51 (2006)
DOI:10.1074/jbc.M608103200
  Sequence
[sgr:SGR_4246]
Reference
2  [PMID:19268377]
  Authors
Le Roes-Hill M, Goodwin C, Burton S
  Title
Phenoxazinone synthase: what's in a name?
  Journal
Trends Biotechnol 27:248-58 (2009)
DOI:10.1016/j.tibtech.2009.01.001
  Sequence
[sgr:SGR_4246]
Other DBs
ExplorEnz - The Enzyme Database: 1.10.3.15
IUBMB Enzyme Nomenclature: 1.10.3.15
ExPASy - ENZYME nomenclature database: 1.10.3.15
BRENDA, the Enzyme Database: 1.10.3.15

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