KEGG   ENZYME: 1.10.3.16Help
Entry
EC 1.10.3.16                Enzyme                                 

Name
dihydrophenazinedicarboxylate synthase;
phzG (gene name)
Class
Oxidoreductases;
Acting on diphenols and related substances as donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
1,4,5a,6,9,10a-hexahydrophenazine-1,6-dicarboxylate:oxygen oxidoreductase
Reaction(IUBMB)
(1) (1R,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylate + O2 = (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate + H2O2 [RN:R11489];
(2) (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate + O2 = (5aS)-5,5a-dihydrophenazine-1,6-dicarboxylate + H2O2 [RN:R11490];
(3) (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1-carboxylate + O2 = (10aS)-10,10a-dihydrophenazine-1-carboxylate + H2O2 [RN:R11491];
(4) (1R)-1,4,5,10-tetrahydrophenazine-1-carboxylate + O2 = (10aS)-5,10-dihydrophenazine-1-carboxylate + H2O2
Reaction(KEGG)
Substrate
(1R,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylate [CPD:C21408];
O2 [CPD:C00007];
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate [CPD:C21409];
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1-carboxylate [CPD:C21411];
(1R)-1,4,5,10-tetrahydrophenazine-1-carboxylate [CPD:C21413]
Product
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate [CPD:C21409];
H2O2 [CPD:C00027];
(5aS)-5,5a-dihydrophenazine-1,6-dicarboxylate [CPD:C21410];
(10aS)-10,10a-dihydrophenazine-1-carboxylate [CPD:C21412];
(10aS)-5,10-dihydrophenazine-1-carboxylate
Comment
Requires FMN. The enzyme, isolated from the bacteria Pseudomonas fluorescens 2-79 and Burkholderia lata 383, is involved in biosynthesis of the reduced forms of phenazine, phenazine-1-carboxylate, and phenazine-1,6-dicarboxylate, where it catalyses multiple reactions.
History
EC 1.10.3.16 created 2016
Pathway
ec00405  Phenazine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01130  Biosynthesis of antibiotics
Orthology
K20262  dihydrophenazinedicarboxylate synthase
Genes
SFW: WN53_03370
ECA: ECA2698(ehpE)
PATR: EV46_13180
PATO: GZ59_18620(ehpE)
LAB: LA76x_3331(phzG)
LAQ: GLA29479_2364(phzG)
PAE: PA1905(phzG2) PA4216(phzG1)
PAEV: N297_1965(pdxH) N297_4348(pdxH)
PAEI: N296_1965(pdxH) N296_4348(pdxH)
PAU: PA14_09410(phzG1) PA14_39880(phzG2)
PAG: PLES_07111(phzG1) PLES_34191(phzG2)
PAF: PAM18_0721(phzG1) PAM18_3137(phzG2)
PNC: NCGM2_2822(phzG1) NCGM2_5461(phzG1)
PAEB: NCGM1900_0743(phzG1) NCGM1900_4584(phzG1)
PAEU: BN889_04687(phzG2_1) BN889_06679(phzG2_2)
PAEC: M802_1963(pdxH) M802_4346(pdxH)
PAEO: M801_1964(pdxH) M801_4214(pdxH)
PFB: VO64_0340
BGU: KS03_4989(phzG)
SALS: SLNWT_0234
STRD: NI25_24415
SALC: C2138_10050(pdxH)
SALD: FVA74_10700(pdxH)
BRI: FDF13_09775(pdxH)
NDA: Ndas_5033
APRE: CNX65_16905(pdxH)
ACTA: C1701_23220(pdxH)
ALO: CRK58743
 » show all
Taxonomy
Reference
1  [PMID:23897464]
  Authors
Xu N, Ahuja EG, Janning P, Mavrodi DV, Thomashow LS, Blankenfeldt W
  Title
Trapped intermediates in crystals of the FMN-dependent oxidase PhzG provide insight into the final steps of phenazine biosynthesis.
  Journal
Acta Crystallogr D Biol Crystallogr 69:1403-13 (2013)
DOI:10.1107/S0907444913008354
Other DBs
ExplorEnz - The Enzyme Database: 1.10.3.16
IUBMB Enzyme Nomenclature: 1.10.3.16
ExPASy - ENZYME nomenclature database: 1.10.3.16
BRENDA, the Enzyme Database: 1.10.3.16

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