KEGG   ENZYME: 1.11.1.29
Entry
EC 1.11.1.29                Enzyme                                 
Name
mycoredoxin-dependent peroxiredoxin;
ahpE (gene name)
Class
Oxidoreductases;
Acting on a peroxide as acceptor;
Peroxidases
Sysname
mycoredoxin:hydroperoxide oxidoreductase
Reaction(IUBMB)
mycoredoxin + ROOH = mycoredoxin disulfide + H2O + ROH
Substrate
mycoredoxin;
ROOH [CPD:C15498]
Product
mycoredoxin disulfide;
H2O [CPD:C00001];
ROH [CPD:C01335]
Comment
Peroxiredoxins (Prxs) are a ubiquitous family of antioxidant proteins. They can be divided into three classes: typical 2-Cys, atypical 2-Cys and 1-Cys peroxiredoxins [1]. The peroxidase reaction comprises two steps centred around a redox-active cysteine called the peroxidatic cysteine. All three peroxiredoxin classes have the first step in common, in which the peroxidatic cysteine attacks the peroxide substrate and is oxidized to S-hydroxycysteine (a sulfenic acid) (see {single/111115a::mechanism}). The second step of the peroxidase reaction, the regeneration of cysteine from S-hydroxycysteine, distinguishes the three peroxiredoxin classes. For typical 2-Cys Prxs, in the second step, the peroxidatic S-hydroxycysteine from one subunit is attacked by the 'resolving' cysteine located in the C-terminus of the second subunit, to form an intersubunit disulfide bond, which is then reduced by one of several cell-specific thiol-containing reductants completing the catalytic cycle. In the atypical 2-Cys Prxs, both the peroxidatic cysteine and its resolving cysteine are in the same polypeptide, so their reaction forms an intrachain disulfide bond. The 1-Cys Prxs conserve only the peroxidatic cysteine, so its regeneration involves direct interaction with a reductant molecule. Mycoredoxin-dependent enzymes are found in Mycobacteria. Following the reduction of the substrate, the sulfenic acid derivative of the peroxidatic cysteine forms a protein mixed disulfide with the N-terminal cysteine of mycoredoxin, which is then reduced by the C-terminal cysteine of mycoredoxin, restoring the peroxiredoxin to active state and resulting in an intra-protein disulfide in mycoredoxin. The disulfide is eventually reduced by mycothiol.
History
EC 1.11.1.29 created 1983 as EC 1.11.1.15, part transferred 2020 to EC 1.11.1.29
Orthology
K24120  mycoredoxin-dependent peroxiredoxin
Genes
RTHLRK53_00420
GNTKP003_05405
PAUUE8A73_034790
BSHILGQ02_03945
EFFskT53_35540
MTURv2238c(ahpE)
MTVRVBD_2238c
MTCMT2298
MRAMRA_2258(ahpE)
MTFTBFG_12267
MTBTBMG_01742
MTKTBSG_01752
MTZTBXG_001724
MTGMRGA327_13820
MTECCDC5079_2075
MTURCFBS_2371(ahpE)
MTLCCDC5180_2047
MTOMTCTRI2_2274(ahpE)
MTDUDA_2238c(ahpE)
MTNERDMAN_2463(ahpE)
MTJJ112_12020
MTUBMT7199_2270
MTUCJ113_15580
MTUEJ114_12000
MTXM943_11595
MTUHI917_15770
MTULTBHG_02190
MTUTHKBT1_2362(ahpE)
MTUUHKBT2_2364(ahpE)
MTQHKBS1_2368(ahpE)
MBOBQ2027_MB2262C(ahpE)
MBBBCG_2255c(ahpE)
MBTJTY_2249(ahpE)
MBMBCGMEX_2243c(ahpE)
MBKK60_023230
MBXBCGT_2061
MAFMAF_22490(ahpE)
MMICRN08_2485
MCEMCAN_22601(ahpE)
MCQBN44_50181(ahpE)
MCVBN43_31480(ahpE)
MCXBN42_40162(ahpE)
MCZBN45_50570(ahpE)
MPAMAP_1991c(ahpE)
MAOMAP4_1834
MAVIRC58_09165
MAVURE97_09170
MAVMAV_2199
MITOCO_21860
MIAOCU_22050
MIDMIP_03087
MYOOEM_19760
MCHIAN480_11310
MIROCQ_20970
MMALCKJ54_09760
MMANMMAN_17950
MSAMycsm_03973
MULMUL_1311(ahpE)
MMCMmcs_3359
MKMMkms_3421
MJLMjls_3370
MMIMMAR_3331(ahpE)
MMAEMMARE11_32320(ahpE)
MMMW7S_10280
MLIMULP_03592(ahpE)
MKNMKAN_03310
MYVG155_18540
MYEAB431_19225
MGOAFA91_18505
MHADB586_09930
MDXBTO20_13125
MSHGMSG_03163
MFJMFLOJ_00530
MSTOMSTO_01190
MSIMMSIM_45230
MSAKMSAS_12120
MKUI2456_16020
MGROFZ046_22610
MXEMYXE_20800
MNVMNVI_45080
MPAGC0J29_18605
MNMMNVM_02300
MGORH0P51_17325
MCOOMCOO_12740
MBAIMB901379_03004
MSEOMSEO_19500
MPSEMPSD_34080
MSHOMSHO_47430
MHEKJMUB5695_02089
MGAUMGALJ_21240
MLJMLAC_22510
MBRDMBRA_35690
MSHJMSHI_10240
MKRMKOR_04770
MDFK0O62_18225
MMAMK3U93_14840
MHOLK3U96_10685
MHERK3U94_09115
MSENK3U95_15960
MPAEK0O64_18590
MSPGF6B93_13930
MOTLTS72_07860
MSMMSMEG_4320
MSGMSMEI_4220
MSBLJ00_21410
MSNLI99_21415
MSHLI98_21420
MVAMvan_3749
MGIMflv_2784
MSPMspyr1_22240
MCBMycch_3271
MNED174_18270
MYNMyAD_17915
MFTXA26_37860
MPHLMPHLCCUG_03384
MVQMYVA_3571
MLLB1R94_10625
MRHMycrhN_4442
MTHN4412656_01793
MHASMHAS_01456
MDUMDUV_08210
MCHTMCHIJ_33710
MDRMDOR_24740
MAUUNCTC10437_03506
MMAGMMAD_35060
MMORMMOR_29510
MFXMFAL_13560
MAICMAIC_34140
MIJMINS_32270
MALVMALV_02350
MTYMTOK_56200
MPSCMPSYJ_30270
MARZMARA_61420
MGADMGAD_03020
MHEVMHEL_60180
MSARMSAR_35240
MANYMANY_19000
MAUBMAUB_50810
MPOFMPOR_39220
MPHUMPHO_18610
MMUCC1S78_009130
MMATMMAGJ_73490
MBOKMBOE_36070
MFGK6L26_13165
MSEIMSEDJ_47170
MFLVNCTC10271_01906
MABMAB_1887
MMVMYCMA_0976
MABBMASS_1874
MABLMMASJCM_1805
MCHEBB28_09720
MIZBAB75_10160
MSTEMSTE_01802
MSAOMYCSP_08160
MSALDSM43276_01643
MJDJDM601_1658(ahpE)
MTER4434518_01570(ahpE)
MMINMMIN_32570
MHIBMHIB_10910
ASDAS9A_1230
MPAKMIU77_06630
CCYCSCMU_23550
NFANFA_16100
NFRERS450000_02550
NCYNOCYR_1665
NBRO3I_010870
NNONONO_c20890
NSLBOX37_07815
NSRNS506_03006(prdX)
NTPCRH09_11665
NOZDMB37_31430
NODFOH10_06535
NAHF5544_14385
NADNCTC11293_02571
NWLNWFMUON74_51830
NIEKV110_10900
NTCKHQ06_15875
NHUH0264_15100
NYALTV02_18035
NGPLTT66_03365
RHARHA1_ro01194
RERRER_36670(ahpE)
REYO5Y_16860
REBXU06_17065
RQIC1M55_18065
ROPROP_09170(ahpE)
ROAPd630_LPD05293
REQREQ_28910
RPYY013_06200
RBYCEJ39_16985
RHBNY08_590
RAVAAT18_15835
RFAA3L23_03839
RHSA3Q41_04403
RHWBFN03_10165
RRZCS378_05485
RHUA3Q40_01116
RHQIM25_20215
RHODAOT96_28385
RRT4535765_03058
RCRNCTC10994_04203
RTMG4H71_01290
RKOJWS14_06195
RGOKYT97_25455
GBRGbro_3116
GPOGPOL_c28800
GORKTR9_3109
GOQACH46_12985
GTABCM27_15975
GOCCXX93_10350
GITC6V83_13360
GRUGCWB2_16190
GOMD7316_00157
GAVC5O27_12020
GODGKZ92_14940
GAMGII34_14890
GPDGII33_14010
GJIH1R19_15080
GOILK459_18925
SPINKV203_06435
TPRTpau_2720
TSMASU32_15165
TOYFO059_12200
SCOSCO2369(SCC8A.27c)
SALBXNR_4528
SMASAVERM_5802(ahpE)
SGRSGR_5132
SGBWQO_09685
SCBSCAB_64321
SSXSACTE_1860
SVLStrvi_7523
SHYSHJG_3862
SHOSHJGH_3627
SRCM271_33455
SMALSMALA_2364
SSOII1A49_14905
SCTSCAT_1479
SCYSCATT_14800
SFASfla_4456
SBHSBI_07476(ahpE)
SVESVEN_2183
SDVBN159_5981
SALSSLNWT_5457
STRPF750_2237
SFISFUL_1950
SCIB446_12390
SALUDC74_2860
SALLSAZ_15685
SLVSLIV_25840
SGUSGLAU_10890
SVTSVTN_11865
STREGZL_06120
SCWTU94_10235
SLDT261_5692
SLCSL103_07055
SXISXIM_12890
STRMM444_11960
STRCAA958_08130
SAMBSAM23877_2438
SPRISPRI_5104
SCZABE83_26100
SCXAS200_30735
SRWTUE45_02959
STRFASR50_11450
SLEsle_48260(sle_48260)
SRNA4G23_01512
SPAVSpa2297_09965
STRTA8713_09255
SCLFBB341_20210
SGSAVL59_38995
STSIA4E84_12365
SKYD0C37_24445
SALWCP975_10630
SGMGCM10017557_57730
SCADDN051_26550
SLSSLINC_2716
SNRSNOUR_28360
SPLULK06_008770
STRDNI25_27220
SNWBBN63_24525
SAUOBV401_15205
SSIAA7J05_25055
SVUB1H20_08730
SPUNBFF78_29190
SGVB1H19_14010
SLAUSLA_1990
SALFSMD44_02621
SALJSMD11_4719
SLXSLAV_25400
STROSTRMOE7_12765
SFKKY5_2445c
SNZDC008_10120
SGEDWG14_05913
SRJSRO_5071
SLKSLUN_11980
SDXC4B68_27850
SGDELQ87_27425
SQZFQU76_08590
SCYAEJ357_13905
SASTCD934_23350
SNQCP978_11410
STIRDDW44_05990
SKACP970_30260
SGZC0216_24805
SVNCP980_23065
SNKCP967_24045
SHAWCEB94_12790
SRKFGW37_10135
SFICEIZ62_23165
SGALCP966_10730
SSPODDQ41_04840
SVRCP971_23305
SPADDVK44_07915
SFYGFH48_27785
SAQUEJC51_15730
SGFHEP81_05374
SCAVCVT27_08595
SSEOD0Z67_08480
SBYH7H31_25615
SRIMCP984_28200
SSUBCP968_22955
SPHVF9278_12525
SCINCP977_10715
SFUGCNQ36_10790
SPACB1H29_25555
STSUB7R87_08530
SCHACP983_30160
SGXH4W23_12280
SCOECP976_13865
SBATG4Z16_24520
SSPBCP982_13935
SNFJYK04_03076(ahpE)
SPLACP981_12250
SBROGQF42_14865
STUIGCM10017668_20170
SATAC5746_13175
SCHFIPT68_11365
SHUNDWB77_05329
SHARHUT13_07155
SCYGS1361_13160
SFEUIM697_09705
SLIAHA039_24365
SDWK7C20_11575
SVDCP969_12965
SCALI6J39_09020
SAUHSU9_009860
SFBCP974_08620
SMOBJ7W19_09785
SPRACP972_10935
SPEUCGZ69_10865
SROIIAG44_28260
SXNIAG42_24655
SGJIAG43_09395
SHKJ2N69_10485
SANUK7396_25035
STRYEQG64_08710
SDDD9753_24725
SACTDMT42_12280
KSKKSE_24810
KABB7C62_08660
KAUB6264_17890
KITCFP65_2207
STRIC7M71_008265
YIALO772_11030
LXLKDY119_02141
PSICJ4E96_13785
LXXLxx12690
LXYO159_13710
LEIFHF024_09760
LSEF1C12_03510
CMICMM_1622(ahpE)
CMSCMS1605
CMCCMN_01602(ahpE)
CMHVO01_07250
CCAPAES38_07810
CZHH9X71_07860
MCHNHCR76_08700
RLARhola_00006540
RPLAA4Z71_03130
AQGHRU87_03175
RTXTI83_05415
RTCAPU90_01060
RTNA6122_1543
RRYC1O28_07080
RIAC7V51_05555
RFSC1I64_04270
RTEGSU10_08615
CUMNI26_06850
CUBBJK06_16035
CUGC1N91_06725
CQFGBG65_17135
MVDAWU67_06605
FRPAX769_13715
AGYATC03_10485
AGMDCE93_07085
AGFET445_12880
AARCG127AT_00695
CARTPA27867_1677
CRYB7495_08190
CPHYB5808_09950
AMINAUMI_14560
AUMAURMO_00726
AUWAURUGA1_00938
MALKMalAC0309_1654(ahpE)
MYLC3E77_07845
MSPOKXZ72_01905
SALCC2138_07250
SALDFVA74_07030
HUMDVJ78_11000
HUWFPZ11_03130
GRYD7I44_17020
LYDD7I47_12370
LYKFLP23_09465
PLAPEAO79_12955
LEULeucomu_07585
LTREVS81_00385
LEBG7066_07565
LDNH9L06_01845
LVIG7068_01545
LLUTK1X41_01100
AGGC1N71_08190
MANTBHD05_09815
HEAHL652_08610
FRNF1C15_00670
GLNF1C58_08785
AGXAGREI_1728(ahpE)
SUBTKPL76_12105
PSEVUSB125703_01680(ahpE)
ARTArth_2462
ARRARUE_c25860
ARMART_1146
AREAL755_14475
AAQAOC05_09165
ARWMB46_09455
ARHAHiyo8_59720
ARZAUT26_12005
ARUASPU41_17995
ARQBWQ92_19585
ARNCGK93_14300
ARXARZXY2_1535
ACRYAC20117_19965
ARTHC3B78_11900
ARTPE5206_10250
AAGINCTC2676_1_01653
AJGKKR91_11055
AAUAAur_2431
PUEFV140_12250
PNVJMY29_11560
ACHAchl_2201
APNAsphe3_23300
PSULAU252_19495
PSNINIBR502771_13345
PSEYGU243_06340
GPRJQN66_07865
KRHKRH_10830
KPLKPaMU14_04735
KFVAS188_12125
KIIKocCE7_05450
KRSEQG70_07795
KODHBK84_05610
KVRCIB50_0002091
SATKSA2016_2370
PAEYKUF55_09950
DNIHX89_08950
DAYFV141_08080
LMOIVV02_13355
YIMJ5M86_06040
ALXLVQ62_14175
XCEXcel_2130
XYLET495_06680
XYAET471_16440
IVAIsova_1913
IDOI598_0296
CETB8281_10980
CCEUCBR64_20750
SKESked_14160
SANWG7063_09055
CFLCfla_2145
CFICelf_2261
CGACelgi_1362
CEZCBP52_06465
CELZE5225_07075
CEJGC089_07305
CELHGXP71_08215
CIRALFM56_07305
OEKFFI11_015400
ICAIntca_2057
JTEASJ30_09265
JLIEXU32_05130
JMEEEW87_005995
TEHGKE56_02905
PHWG7075_08140
PEIH9L10_04665
SERJSGUI_0121
SERWFY030_09120
ORNDV701_01605
ORZFNH13_12155
BCAUI6G59_12225
DCOSAMEA4475696_1558
AUSIPK37_16760
GYUFE374_09495
HALTIM660_10760
DEMLGT36_003885
MIKFOE78_17270
MICGGJV80_02495
NCANoca_1996
NDKI601_3713
NOYEXE57_04305
NOIFCL41_10710
NOOFE634_08430
NDPE2C04_11325
NSNEXE58_15125
NBEBack2_26670
NANOG5V58_08350
NMESH9L09_01960
NAROCFH99_15860
NPIG7071_12085
NAQUENKNEFLB_02671(ahpE)
NROK8W59_06310
NOQLN652_20925
PSIMKR76_17220
AERAERYTH_11155
AEZC3E78_06380
AEBC6I20_04925
AEFGEV26_06150
MUZH4N58_07155
KQIF1D05_33025
TFUTfu_1964
THAONI17_012075
NDANdas_2943
NALB005_5482
NGVCDO52_18520
STRREKD16_15060
SAIUJ4H86_26685
TCUTcur_1648
ACTWF7P10_07015
AGRAAGRA3207_001526
SROSros_7040
NOABKM31_58420
NOWGBF35_37980
NCXNocox_30940
NGNLCN96_42255
TBITbis_2356
FRAFrancci3_3053
FREFranean1_1864
FRIFraEuI1c_1850
FALFRAAL5045(bcp)
FSYFsymDg_2520
NMLNamu_2056
NAKEH165_08560
GOBGobs_3502
BSDBLASA_1981
MMARMODMU_1855
SENSACE_1534(ahpE)
SACGFDZ84_19840
SVISvir_10330
SACCEYD13_05615
AMDAMED_2115(ahpC)
AMNRAM_10750
AMMAMES_2098(ahpC)
AMZB737_2099(ahpC)
AOIAORI_5798(ahpC)
AJAAJAP_10475
AMQAMETH_5175(ahpC)
AMYCCU254_08615
AMYBBKN51_22515
AABA4R43_18020
AMYYYIM_12040
AORISD37_28385
AACDLWP59_31035
PDXPsed_2143
PSEAWY02_08495
PSEEFRP1_17900
PSEHXF36_06035
PSEQAD006_25345
PECQAD017_04935
PHHAFB00_21720
PAUTPdca_20680(ahpC)
PBROHOP40_17535
APRECNX65_06605
AMIAmir_1336
SESPBN6_15850(ahpC)
SSYIEKG83_07705
KALKALB_1852
KPHYAOZ06_13205
LEDBBK82_02710
AHMTL08_22145
ACTIUA75_25920
ACADUA74_25335
AHGAHOG_23270
ACTAC1701_05940
ALOCRK55644
PMADBAY61_07350
SACEGIY23_07180
STPStrop_3368
SAQSare_3608
MAUMicau_4697
MILML5_3600
MICBMicB006_3708
MTUACSH63_01040
MICHFJK98_24840
MTEMGCE86_17935
MCABHXZ27_23720
MSAGGCM10017556_09100(ahpC)
VMAVAB18032_27711
MCRAID554_23065
ASEACPL_1535(ahpE)
AMSAMIS_13210
ACTNL083_1778(ahpE)
AFSAFR_08800
ACTSACWT_1414
AIHAiant_57800(ahpC)
PLKCIK06_21250
PLABC6361_33705
PLATC6W10_27370
PFLAPflav_054380(ahpC)
PSUUPsuf_014320(ahpC)
VERHUT12_25365
ATLAthai_20240(ahpC)
ASERAsera_54370
PRYPrubr_05880(ahpC)
CATICS0771_75490(ahpC)
DVCDvina_45215
CAICaci_1560
SNASnas_4792
NAVJQS30_10345
GLYK3N28_02620
ASLANCTC11923_01225
AEYCDG81_06485
PLANA1s21148_01575
PLAKA1s21155_01450
PLIMPHILAsVB114_05660
PSUFA1sIA56_01385
PVSA1sIA79_01290
PVNA7sIIA15_01755
ABAMB1s21122_01540
NHIB1s21160_01350
NABB1sIIB91_01240
EKEEK0264_02530
ABAIIMCC26256_111987
ATQGH723_07305
LCREPla8534_57040(bcp_3)
AAGGETAA8_53910(bcp_5)
MRIMal4_24650
SDYNMal52_57480
TSPHKIH39_16195 KIH39_17245
HAADMW046_16805
HALXM0R89_15715
 » show all
Reference
1  [PMID:12517450]
  Authors
Wood ZA, Schroder E, Robin Harris J, Poole LB.
  Title
Structure, mechanism and regulation of peroxiredoxins.
  Journal
Trends Biochem Sci 28:32-40 (2003)
DOI:10.1016/S0968-0004(02)00003-8
Reference
2  [PMID:19737009]
  Authors
Hugo M, Turell L, Manta B, Botti H, Monteiro G, Netto LE, Alvarez B, Radi R, Trujillo M
  Title
Thiol and sulfenic acid oxidation of AhpE, the one-cysteine peroxiredoxin from Mycobacterium tuberculosis: kinetics, acidity constants, and conformational dynamics.
  Journal
Biochemistry 48:9416-26 (2009)
DOI:10.1021/bi901221s
  Sequence
[mtu:Rv2238c]
Reference
3  [PMID:24379404]
  Authors
Hugo M, Van Laer K, Reyes AM, Vertommen D, Messens J, Radi R, Trujillo M
  Title
Mycothiol/mycoredoxin 1-dependent reduction of the peroxiredoxin AhpE from Mycobacterium tuberculosis.
  Journal
J Biol Chem 289:5228-39 (2014)
DOI:10.1074/jbc.M113.510248
  Sequence
[mtu:Rv2238c]
Reference
4  [PMID:27417938]
  Authors
Kumar A, Balakrishna AM, Nartey W, Manimekalai MSS, Gruber G
  Title
Redox chemistry of Mycobacterium tuberculosis alkylhydroperoxide reductase E (AhpE): Structural and mechanistic insight into a mycoredoxin-1 independent reductive pathway of AhpE via mycothiol.
  Journal
Free Radic Biol Med 97:588-601 (2016)
DOI:10.1016/j.freeradbiomed.2016.07.007
  Sequence
[mtu:Rv2238c]
Reference
5  [PMID:27471753]
  Authors
Pedre B, van Bergen LA, Pallo A, Rosado LA, Dufe VT, Molle IV, Wahni K, Erdogan H, Alonso M, Proft FD, Messens J
  Title
The active site architecture in peroxiredoxins: a case study on Mycobacterium tuberculosis AhpE.
  Journal
Chem Commun (Camb) 52:10293-6 (2016)
DOI:10.1039/c6cc02645a
  Sequence
[mtu:Rv2238c]
Other DBs
ExplorEnz - The Enzyme Database: 1.11.1.29
IUBMB Enzyme Nomenclature: 1.11.1.29
ExPASy - ENZYME nomenclature database: 1.11.1.29
BRENDA, the Enzyme Database: 1.11.1.29

DBGET integrated database retrieval system