Entry
Name
iodide peroxidase;
thyroid peroxidase;
iodoperoxidase (heme type);
iodide peroxidase-tyrosine iodinase;
thyroperoxidase;
tyrosine iodinase;
TPO;
iodinase
Class
Oxidoreductases;
Acting on a peroxide as acceptor;
Peroxidases
BRITE hierarchy
Sysname
iodide:hydrogen-peroxide oxidoreductase
Reaction(IUBMB)
(1) 2 iodide + H2O2 + 2 H+ = diiodine + 2 H2O [RN:
R02810 ];
(2) [thyroglobulin]-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3-iodo-L-tyrosine + 2 H2O;
(3) [thyroglobulin]-3-iodo-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3,5-diiodo-L-tyrosine + 2 H2O;
(4) 2 [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-L-thyroxine + [thyroglobulin]-aminoacrylate + 2 H2O [RN:
R08523 ];
(5) [thyroglobulin]-3-iodo-L-tyrosine + [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-3,5,3'-triiodo-L-thyronine + [thyroglobulin]-aminoacrylate + 2 H2O [RN:
R08522 ]
Reaction(KEGG)
Substrate
iodide [CPD:
C00708 ];
H2O2 [CPD:
C00027 ];
H+ [CPD:
C00080 ];
[thyroglobulin]-L-tyrosine [CPD:
C16733 ];
[thyroglobulin]-3-iodo-L-tyrosine [CPD:
C16732 ];
[thyroglobulin]-3,5-diiodo-L-tyrosine [CPD:
C16731 ]
Product
diiodine [CPD:
C01382 ];
H2O [CPD:
C00001 ];
[thyroglobulin]-3-iodo-L-tyrosine [CPD:
C16732 ];
[thyroglobulin]-3,5-diiodo-L-tyrosine [CPD:
C16731 ];
[thyroglobulin]-L-thyroxine [CPD:
C16729 ];
[thyroglobulin]-aminoacrylate [CPD:
C16734 ];
[thyroglobulin]-3,5,3'-triiodo-L-thyronine [CPD:
C16730 ]
Comment
Thyroid peroxidase catalyses the biosynthesis of the thyroid hormones L-thyroxine and triiodo-L-thyronine. It catalyses both the iodination of tyrosine residues in thyroglobulin (forming mono- and di-iodinated forms) and their coupling to form either L-thyroxine or triiodo-L-thyronine.
History
EC 1.11.1.8 created 1961, modified 2012
Pathway
Orthology
Genes
NLE : 100600125(TPO) 115834294
PMUA : 114593754(TPO) 114600521
PRAF : 128410067(TPO) 128417614
ZVI : 118078350 118082781(TPO)
HCG : 128339342 128340645(TPO)
GJA : 107108843 107120374(TPO)
STOW : 125426778 125439527(TPO)
EMC : 129323482 129324144(TPO)
DRE : 100331172(epx) 569363(tpo)
SRX : 107707791(tpo) 107732259
SANH : 107676453 107678715(tpo) 107701038
CCAR : 109084952 109099142
CAUA : 113042938 113085118(tpo)
CGIB : 128013963 128031820
PTET : 122330629(epx) 122334382(tpo)
LROH : 127156758(epx) 127179243(tpo)
OMC : 131523159(tpo) 131534985(epx)
PPRM : 120471728(epx) 120473217 120473261
RKG : 130075798(tpo) 130106479(epx)
MAMB : 125247940(epx) 125269309(tpo)
CIDE : 127498092 127507673
CERY : 137006300(tpo) 137015584(epx)
MASI : 127435674 127455176
TROS : 130550078(epx) 130559836 130560167
TDW : 130414644(epx) 130436790(tpo)
MANU : 129417025(tpo) 129433472(epx)
IPU : 108269580 108274988(epx)
IFU : 128612578(tpo) 128617734(epx)
PHYP : 113529680 113538844(tpo)
SMEO : 124389670(tpo) 124392946(epx)
TFD : 113653966(epx) 113659460(tpo)
TVC : 132852534(tpo) 132856864(epx)
TRN : 134321707(epx) 134325721(tpo)
AMEX : 103026466(epx) 103032699(tpo)
CMAO : 118811861(epx) 118823206(tpo)
EEE : 113572650(tpo) 113583564
CHAR : 105892418(epx) 105909411(tpo)
SPIL : 134068056(tpo) 134093465(epx)
TRU : 101062981 101079869 115247027
TFS : 130536010(epx) 130540092(tpo)
TNG : GSTEN00010284G001 GSTEN00012455G001
LCO : 104928854 104934649(tpo)
TBEN : 117486655 117491490(epx)
CGOB : 115009405 115027547(tpo)
PGEO : 117448489 117468095(tpo)
GACU : 117538992(epx) 117542389
EMAC : 134879856(epx) 134882017(tpo)
ELY : 117261785(epx) 117267275
EFO : 125886852(epx) 125901384(tpo)
PLEP : 121950093(epx) 121953636(tpo)
SLUC : 116038497(epx) 116042521(tpo)
ECRA : 117935626(tpo) 117949679(epx)
ESP : 116669921(tpo) 116694341
PFLV : 114547036(tpo) 114560272
GAT : 120808678(epx) 120832611(tpo)
PPUG : 119195992(epx) 119227498(tpo)
CLUM : 117731997(epx) 117751548(tpo)
PSWI : 130195613(epx) 130201641
CVE : 137877949 137881678(epx)
MSAM : 119893789(epx) 119910842(tpo)
SCHU : 122862127(tpo) 122875391(epx)
CUD : 121514495(epx) 121526598
SPUL : 138591245(tpo) 138612682(epx)
LMIX : 132972597(epx) 132993189(tpo)
ALAT : 119005006(tpo) 119024167(epx)
ASTR : 137593770(epx) 137611202(tpo)
MZE : 101470734(tpo) 101472649
ONL : 100692514 100700906(tpo)
OAU : 116326276(epx) 120434955(tpo)
OLA : 101167362 105356959(tpo)
OML : 112143527(tpo) 112152280(epx)
CSAI : 133444161(epx) 133462180
XHE : 116709035 116709593 116732146
PRET : 103460527 103466494
PFOR : 103141318 103154181(tpo)
PLAI : 106953134(tpo) 106964663
PMEI : 106923933(tpo) 106933097
GAF : 122835368(epx) 122845674(tpo)
PPRL : 129369558(epx) 129374765
CTUL : 119787434(epx) 119797839(tpo)
GMU : 124858077(epx) 124884924(tpo)
NFU : 107388669 107392598(tpo)
KMR : 108230067(epx) 108241739(tpo)
ALIM : 106521174 106521219(tpo)
NWH : 119416466(epx) 119427722(tpo)
AOCE : 111562581(tpo) 111584102
EJA : 138206282(epx) 138212549(tpo)
BFRE : 138626549(epx) 138633479(tpo)
MCEP : 125014719(epx) 125023792(tpo)
CSEM : 103379991(tpo) 103380299
POV : 109624077 109627948(tpo)
SSEN : 122776537(epx) 122783162(tpo)
HHIP : 117756338 117762621(epx)
HSP : 118109462(epx) 118116664(tpo)
PPLT : 128444133(epx) 128450360(tpo)
SMAU : 118286573(sntg2) 118315245(epx)
LCF : 108886978(epx) 108897943
SDU : 111226032(tpo) 111229744
SLAL : 111666321 111666895 111670224(tpo)
XGL : 120792877(epx) 120795322(tpo)
SSCV : 125970095(epx) 125981320(tpo)
SBIA : 133501708(epx) 133513472(tpo)
PEE : 133402397(epx) 133412463(tpo)
PTAO : 133478512(epx) 133487880(tpo)
BPEC : 110155374(tpo) 110169467
MALB : 109965011(tpo) 109972931
BSPL : 114848308(tpo) 114857330(epx)
SSCO : 133982501(epx) 134000449(tpo)
SASA : 106584777 106611389(tpo)
STRU : 115161765 115192923
OTW : 112237628(epx) 112256946(tpo)
OMY : 110504252(tpo) 110522697(epx)
OGO : 124037374(epx) 124046982(tpo)
OKE : 118369032(tpo) 118377954(epx)
OMM : 135526888(tpo) 135533365(epx)
SALP : 112069494(tpo) 112070266
SNH : 120022964 120022991 120051654(epx)
CCLU : 121540756(epx) 121544226(tpo)
ELS : 105028745(tpo) 105029701
PKI : 111848005 111857914(tpo)
AANG : 118215539(epx) 118234749(tpo)
AROT : 135241772(epx) 135252679(tpo)
CCOG : 133111088(epx) 133122436(tpo)
PSPA : 121294970(epx) 121317659(tpo)
ARUT : 117411246(tpo) 117432329(epx)
CMK : 103175078 103185975(tpo)
RTP : 109918711(tpo) 109928222
CPLA : 122548625(tpo) 122557877
HOC : 132824289 132835956(tpo)
LERI : 129696869(tpo) 129705776
CIN : 100183311 100183818 445593
TCF : 131881866 131882001 131882614 131891773
» show all
Taxonomy
Reference
Authors
CUNNINGHAM BA, KIRKWOOD S.
Title
Enzyme systems concerned with the synthesis of monoiodotyrosine. III. Ion requirements of the soluble system.
Journal
J Biol Chem 236:485-9 (1961)
Reference
Authors
HOSOYA T, KONDO Y, UI N.
Title
Peroxidase activity in thyroid gland and partial purification of the enzyme.
Journal
Reference
Authors
Coval ML, Taurog A.
Title
Purification and iodinating activity of hog thyroid peroxidase.
Journal
J Biol Chem 242:5510-23 (1967)
Reference
Authors
Gavaret JM, Cahnmann HJ, Nunez J
Title
Thyroid hormone synthesis in thyroglobulin. The mechanism of the coupling reaction.
Journal
J Biol Chem 256:9167-73 (1981)
Reference
Authors
Ohtaki S, Nakagawa H, Nakamura M, Yamazaki I
Title
One- and two-electron oxidations of tyrosine, monoiodotyrosine, and diiodotyrosine catalyzed by hog thyroid peroxidase.
Journal
J Biol Chem 257:13398-403 (1982)
Reference
Authors
Magnusson RP, Taurog A, Dorris ML
Title
Mechanism of iodide-dependent catalatic activity of thyroid peroxidase and lactoperoxidase.
Journal
J Biol Chem 259:197-205 (1984)
Reference
Authors
Virion A, Courtin F, Deme D, Michot JL, Kaniewski J, Pommier J
Title
Spectral characteristics and catalytic properties of thyroid peroxidase-H2O2 compounds in the iodination and coupling reactions.
Journal
Reference
Authors
Rawitch AB, Pollock G, Yang SX, Taurog A
Title
Thyroid peroxidase glycosylation: the location and nature of the N-linked oligosaccharide units in porcine thyroid peroxidase.
Journal
Sequence
Reference
Authors
Sun W, Dunford HB
Title
Kinetics and mechanism of the peroxidase-catalyzed iodination of tyrosine.
Journal
Reference
Authors
Taurog A, Dorris ML, Doerge DR
Title
Mechanism of simultaneous iodination and coupling catalyzed by thyroid peroxidase.
Journal
Reference
Authors
Ruf J, Carayon P
Title
Structural and functional aspects of thyroid peroxidase.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 1.11.1.8
ExPASy - ENZYME nomenclature database: 1.11.1.8