Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
linoleate + O2 = (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate [RN:
The product (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate, is converted, more slowly, into (9Z,11E)-(13R)-13-hydroperoxyoctadeca-9,11-dienoate. The enzyme from the fungus Gaeumannomyces graminis requires Mn2+. It also acts on alpha-linolenate, whereas gamma-linolenate is a poor substrate. Oleate and arachidonate are not substrates.
EC 184.108.40.206 created 2000
K21794 linoleate 11-lipoxygenase
Hamberg M, Su C, Oliw E.
Manganese lipoxygenase. Discovery of a bis-allylic hydroperoxide as product and intermediate in a lipoxygenase reaction.
Oliw EH, Su C, Skogstrom T, Benthin G.
Analysis of novel hydroperoxides and other metabolites of oleic, linoleic, and linolenic acids by liquid chromatography-mass spectrometry with ion trap MSn.
Su C, Oliw EH
Manganese lipoxygenase. Purification and characterization.