Entry
Name
carotenoid isomerooxygenase;
ninaB (gene name)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
BRITE hierarchy
Sysname
zeaxanthin:oxygen 15,15'-oxidoreductase (bond-cleaving, cis-isomerizing)
Reaction(IUBMB)
zeaxanthin + O2 = (3R)-11-cis-3-hydroxyretinal + (3R)-all-trans-3-hydroxyretinal [RN:
R10192 ]
Reaction(KEGG)
Substrate
Product
(3R)-11-cis-3-hydroxyretinal [CPD:
C18195 ];
(3R)-all-trans-3-hydroxyretinal [CPD:
C18196 ]
Comment
The enzyme, characterized from the moth Galleria mellonella and the fruit fly Drosophila melanogaster, is involved in the synthesis of retinal from dietary caroteoids in insects. The enzyme accepts different all-trans carotenoids, including beta-carotene, alpha-carotene and lutein, and catalyses the symmetrical cleavage of the carotenoid and the simultaneous isomerization of only one of the products to a cis configuration. When the substrate is hydroxylated only in one side (as in cryptoxanthin), the enzyme preferentially isomerizes the hydroxylated part of the molecule.
History
EC 1.13.11.65 created 2012 as EC 1.14.13.164, transferred 2012 to EC 1.13.11.65
Orthology
K18048 carotenoid isomerooxygenase
Genes
DSI : Dsimw501_GD18923(Dsim_GD18923)
ASUA : 134205983 134222334
LLL : 129789653 129790491 129796678
CFO : 105250638 105253782 109609955
AGIF : 122849754 122850321
TMOL : 138138150(ninaB) 138138151 138138763
PPYR : 116158920 116158994 116159061
DPL : KGM_212590 KGM_212591 KGM_212592
BANY : 112048988 112048993 112048997
MJU : 123869307 123869308 123869309 123869313 123869456
NIQ : 126771420 126771421 126771423 126771431
VCD : 124531853 124532026 124532164 124532165
MCIX : 123655557 123655888 123655936
PMAC : 106711577 106711578
PPOT : 106106397 106106755
PRAP : 111001438 111001440
PNAP : 125052278 125052279
CCRC : 123693611 123694083
LSIN : 126971605 126971606 126971607
AAGE : 121739667 121739668
ACOO : 126837815 126845101 126846653
PVM : 113809949 113815366 113815367 113821294 113827985
PJA : 122243363 122245927 122247943 122262617 122262618
PCHN : 125025759 125043010 125044261 125044432
PMOO : 119576962 119579587 119579588 119587833
HAME : 121853934 121878637 121879857
PCLA : 123758838 123765072 123765221 138369803
PTRU : 123508703 123512187 123518893
ESN : 126983888 126998754 127008516
MNZ : 135217174 135219221 135220864 135223800
MRJ : 136833339 136835926 136842776 136848687
HAZT : 108664736 108664738 108664739 108668518 108672094
PPOI : 119093003 119093008
RSAN : 119394925 119395109
» show all
Taxonomy
Reference
Authors
Oberhauser V, Voolstra O, Bangert A, von Lintig J, Vogt K
Title
NinaB combines carotenoid oxygenase and retinoid isomerase activity in a single polypeptide.
Journal
Sequence
Other DBs