KEGG   ENZYME: 1.13.11.65
Entry
EC 1.13.11.65               Enzyme                                 

Name
carotenoid isomerooxygenase;
ninaB (gene name)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
Sysname
zeaxanthin:oxygen 15,15'-oxidoreductase (bond-cleaving, cis-isomerizing)
Reaction(IUBMB)
zeaxanthin + O2 = (3R)-11-cis-3-hydroxyretinal + (3R)-all-trans-3-hydroxyretinal [RN:R10192]
Reaction(KEGG)
R10192
Substrate
zeaxanthin [CPD:C06098];
O2 [CPD:C00007]
Product
(3R)-11-cis-3-hydroxyretinal [CPD:C18195];
(3R)-all-trans-3-hydroxyretinal [CPD:C18196]
Comment
The enzyme, characterized from the moth Galleria mellonella and the fruit fly Drosophila melanogaster, is involved in the synthesis of retinal from dietary caroteoids in insects. The enzyme accepts different all-trans carotenoids, including beta-carotene, alpha-carotene and lutein, and catalyses the symmetrical cleavage of the carotenoid and the simultaneous isomerization of only one of the products to a cis configuration. When the substrate is hydroxylated only in one side (as in cryptoxanthin), the enzyme preferentially isomerizes the hydroxylated part of the molecule.
History
EC 1.13.11.65 created 2012 as EC 1.14.13.164, transferred 2012 to EC 1.13.11.65
Orthology
K18048  carotenoid isomerooxygenase
Genes
DME: Dmel_CG9347(ninaB)
DER: 6553504
DSE: 6606493
DSI: Dsimw501_GD18923(Dsim_GD18923)
DYA: Dyak_GE26290
DAN: 6499668
DSR: 110191325
DPO: Dpse_GA21719
DPE: 6587410
DMN: 108156831
DWI: 6651436
DAZ: 108608798
DNV: 108660197
DHE: 111594412
DVI: 6635002
MDE: 101891299
LCQ: 111689802
AAG: 5577639
AALB: 109416057
AME: 410521
BIM: 100748194
BTER: 100650576
CCAL: 108622230
OBB: 114874170
SOC: 105207446
MPHA: 105838552
AEC: 105152891
ACEP: 105627461
PBAR: 105429637
HST: 105189528
LHU: 105678748
PGC: 109851872
OBO: 105284924
PCF: 106786697
NVI: 100121134
CSOL: 105366669
MDL: 103579989
TCA: 655806
DPA: 109546877
ATD: 109599337
NVL: 108568592
BMOR: 101740058(ninaB-1)
BMAN: 114250535
HAW: 110384440
TNL: 113497779
PXY: 105388704
API: 100168974
DNX: 107165925
AGS: 114124246
RMD: 113553170
BTAB: 109031571
CLEC: 106674446
ZNE: 110839939
FCD: 110854110
 » show all
Reference
1  [PMID:19020100]
  Authors
Oberhauser V, Voolstra O, Bangert A, von Lintig J, Vogt K
  Title
NinaB combines carotenoid oxygenase and retinoid isomerase activity in a single polypeptide.
  Journal
Proc Natl Acad Sci U S A 105:19000-5 (2008)
DOI:10.1073/pnas.0807805105
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.65
IUBMB Enzyme Nomenclature: 1.13.11.65
ExPASy - ENZYME nomenclature database: 1.13.11.65
BRENDA, the Enzyme Database: 1.13.11.65

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