KEGG   ENZYME: 1.13.11.85
Entry
EC 1.13.11.85               Enzyme                                 

Name
exo-cleaving rubber dioxygenase;
roxA (gene name);
heme-dependent rubber oxygenase (ambiguous)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
Sysname
cis-1,4-polyisoprene:oxygen dioxygenase [(4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal-forming]
Reaction(IUBMB)
cis-1,4-polyisoprene + n O2 = n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal [RN:R11999]
Reaction(KEGG)
R11999
Substrate
cis-1,4-polyisoprene [CPD:C02321];
O2 [CPD:C00007]
Product
(4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal [CPD:C21834]
Comment
The enzyme, studied mainly from the bacterium Xanthomonas sp. 35Y, catalyses the cleavage of the double bonds in natural and synthetic rubber (cis-1,4-polyisoprene polymers), generating ends that contain ketone and aldehyde groups. The enzyme from Xanthomonas sp. 35Y contains two c-type cytochromes. It attacks the substrate from its end, producing a single product of 15 carbons.
History
EC 1.13.11.85 created 2018
Orthology
K22690  exo-cleaving rubber dioxygenase
Genes
KAK: Kalk_04905
SOK: D0B54_19000
METP: C1M51_13405
RGU: A4W93_07150
MYM: A176_007569
MFB: MFUL124B02_27385
CCX: COCOR_02166
MFU: LILAB_11505
MMAS: MYMAC_006684
AVM: JQX13_05245
HOH: Hoch_1661
 » show all
Reference
1  [PMID:16348100]
  Authors
Tsuchii A, Takeda K
  Title
Rubber-degrading enzyme from a bacterial culture.
  Journal
Appl Environ Microbiol 56:269-74 (1990)
DOI:10.1128/AEM.56.1.269-274.1990
Reference
2  [PMID:12855168]
  Authors
Jendrossek D, Reinhardt S
  Title
Sequence analysis of a gene product synthesized by Xanthomonas sp. during growth  on natural rubber latex.
  Journal
FEMS Microbiol Lett 224:61-5 (2003)
DOI:10.1016/S0378-1097(03)00424-5
Reference
3  [PMID:15574940]
  Authors
Braaz R, Fischer P, Jendrossek D
  Title
Novel type of heme-dependent oxygenase catalyzes oxidative cleavage of rubber (poly-cis-1,4-isoprene).
  Journal
Appl Environ Microbiol 70:7388-95 (2004)
DOI:10.1128/AEM.70.12.7388-7395.2004
Reference
4  [PMID:15870336]
  Authors
Braaz R, Armbruster W, Jendrossek D
  Title
Heme-dependent rubber oxygenase RoxA of Xanthomonas sp. cleaves the carbon backbone of poly(cis-1,4-Isoprene) by a dioxygenase mechanism.
  Journal
Appl Environ Microbiol 71:2473-8 (2005)
DOI:10.1128/AEM.71.5.2473-2478.2005
  Sequence
Reference
5  [PMID:23922395]
  Authors
Seidel J, Schmitt G, Hoffmann M, Jendrossek D, Einsle O
  Title
Structure of the processive rubber oxygenase RoxA from Xanthomonas sp.
  Journal
Proc Natl Acad Sci U S A 110:13833-8 (2013)
DOI:10.1073/pnas.1305560110
  Sequence
Reference
6  [PMID:24907333]
  Authors
Birke J, Jendrossek D
  Title
Rubber oxygenase and latex clearing protein cleave rubber to different products and use different cleavage mechanisms.
  Journal
Appl Environ Microbiol 80:5012-20 (2014)
DOI:10.1128/AEM.01271-14
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.85
IUBMB Enzyme Nomenclature: 1.13.11.85
ExPASy - ENZYME nomenclature database: 1.13.11.85
BRENDA, the Enzyme Database: 1.13.11.85

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