KEGG   ENZYME: 1.13.11.87
Entry
EC 1.13.11.87               Enzyme                                 

Name
endo-cleaving rubber dioxygenase;
latex clearing protein;
lcp (gene name);
roxB (gene name)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
Sysname
cis-1,4-polyisoprene:oxygen dioxygenase (endo-cleaving)
Reaction(IUBMB)
Cleavage of cis-1,4-polyisoprene polymers into a mixture of compounds, including a C20 compound ((4Z,8Z,12Z,16Z,20Z,24Z)-4,8,12,16,20,24-hexamethyl-28-oxononacosa-4,8,12,16,20,24-hexaenal), a C25 compound ((4Z,8Z,12Z,16Z,20Z)-4,8,12,16,20-pentamethyl-24-oxopentacosa-4,8,12,16,20-pentaenal), a C30 compound ((4Z,8Z,12Z,16Z)-4,8,12,16-tetramethyl-20-oxohenicosa-4,8,12,16-tetraenal), and larger isoprenologes such as C35, C40, C45, and higher analogues.
Comment
The enzyme catalyses the cleavage of the double bonds in natural and synthetic rubber, producing a mixture of C20, C25, C30, and higher oligo-isoprenoids with ketone and aldehyde groups at their ends. Two unrelated bacterial enzymes are known to possess this activity - the enzyme from Streptomyces sp. K30 (Lcp) contains a b-type cytochrome, while the enzyme from Xanthomonas sp. 35Y, (RoxB) contains two c-type cytochromes. Both enzymes attack the substrate at random locations, and are not able to cleave the C35 or smaller products into shorter fragments.
History
EC 1.13.11.87 created 2018
Orthology
K23117  endo-cleaving rubber dioxygenase
K23118  endo-cleaving rubber dioxygenase
Genes
KAK: Kalk_09255
SOK: D0B54_06115 D0B54_06125 D0B54_14040 D0B54_15470 D0B54_18995 D0B54_19015 D0B54_23695
SINI: GT972_12125 GT972_12265
RGU: A4W93_01825
MYM: A176_005221 A176_005491
MFB: MFUL124B02_11685 MFUL124B02_36925
CCX: COCOR_02837
MRM: A7982_05515
HOH: Hoch_1446
ASD: AS9A_0019
NFA: NFA_21740
NFR: ERS450000_01925(lcp_2)
NNO: NONO_c40940(lcp)
NAD: NCTC11293_02479(lcp_1)
RRT: 4535765_00883(lcp_1)
RCR: NCTC10994_01110(lcp_1)
GPO: GPOL_174p00150(lcp2) GPOL_c48310(lcp1)
GOR: KTR9_1229
GRU: GCWB2_06690(lcp2)
GOM: D7316_00417(lcp_1)
SCO: SCO6780(SC6A5.29)
SFA: Sfla_5664
SLV: SLIV_04620(lcp)
SLE: sle_64270(sle_64270) sle_64280(sle_64280)
TCU: Tcur_0105
SRO: Sros_5156
NCX: Nocox_19325(lcp1) Nocox_19330(lcp2) Nocox_38035(lcp4)
AMD: AMED_3299
AMN: RAM_16785
AMM: AMES_3263
AMZ: B737_3263
AJA: AJAP_12695(lcp1) AJAP_12715(lcp2)
AMYY: YIM_18850(lcp1) YIM_34625(lcp2)
AORI: SD37_24020
KAL: KALB_2392
 » show all
Reference
1  [PMID:16348100]
  Authors
Tsuchii A, Takeda K
  Title
Rubber-degrading enzyme from a bacterial culture.
  Journal
Appl Environ Microbiol 56:269-74 (1990)
DOI:10.1128/AEM.56.1.269-274.1990
Reference
2  [PMID:12855168]
  Authors
Jendrossek D, Reinhardt S
  Title
Sequence analysis of a gene product synthesized by Xanthomonas sp. during growth  on natural rubber latex.
  Journal
FEMS Microbiol Lett 224:61-5 (2003)
DOI:10.1016/S0378-1097(03)00424-5
Reference
3  [PMID:15574940]
  Authors
Braaz R, Fischer P, Jendrossek D
  Title
Novel type of heme-dependent oxygenase catalyzes oxidative cleavage of rubber (poly-cis-1,4-isoprene).
  Journal
Appl Environ Microbiol 70:7388-95 (2004)
DOI:10.1128/AEM.70.12.7388-7395.2004
Reference
4  [PMID:15870336]
  Authors
Braaz R, Armbruster W, Jendrossek D
  Title
Heme-dependent rubber oxygenase RoxA of Xanthomonas sp. cleaves the carbon backbone of poly(cis-1,4-Isoprene) by a dioxygenase mechanism.
  Journal
Appl Environ Microbiol 71:2473-8 (2005)
DOI:10.1128/AEM.71.5.2473-2478.2005
Reference
5  [PMID:23922395]
  Authors
Seidel J, Schmitt G, Hoffmann M, Jendrossek D, Einsle O
  Title
Structure of the processive rubber oxygenase RoxA from Xanthomonas sp.
  Journal
Proc Natl Acad Sci U S A 110:13833-8 (2013)
DOI:10.1073/pnas.1305560110
Reference
6  [PMID:24907333]
  Authors
Birke J, Jendrossek D
  Title
Rubber oxygenase and latex clearing protein cleave rubber to different products and use different cleavage mechanisms.
  Journal
Appl Environ Microbiol 80:5012-20 (2014)
DOI:10.1128/AEM.01271-14
  Sequence
Reference
7  [PMID:28500046]
  Authors
Birke J, Rother W, Jendrossek D
  Title
RoxB Is a Novel Type of Rubber Oxygenase That Combines Properties of Rubber Oxygenase RoxA and Latex Clearing Protein (Lcp).
  Journal
Appl Environ Microbiol 83:e00721-17 (2017)
DOI:10.1128/AEM.00721-17
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.87
IUBMB Enzyme Nomenclature: 1.13.11.87
ExPASy - ENZYME nomenclature database: 1.13.11.87
BRENDA, the Enzyme Database: 1.13.11.87

DBGET integrated database retrieval system