KEGG   ENZYME: 1.13.11.88Help
Entry
EC 1.13.11.88               Enzyme                                 

Name
isoeugenol monooxygenase;
iem (gene name)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
BRITE hierarchy
Sysname
isoeugenol:oxygen 7,8-oxidoreductase (bond-cleaving)
Reaction(IUBMB)
isoeugenol + O2 = vanillin + acetaldehyde [RN:R12302]
Reaction(KEGG)
Substrate
isoeugenol [CPD:C10469];
O2 [CPD:C00007]
Product
vanillin [CPD:C00755];
acetaldehyde [CPD:C00084]
Comment
Contains Fe(II). The enzyme, charcterised from the bacteria Pseudomonas putida and Pseudomonas nitroreducens, catalyses the epoxidation of the double bond in the side chain of isoeugenol, followed by a second oxygenation and cleavage of the side chain in the form of acetaldehyde.
History
EC 1.13.11.88 created 2019
Orthology
K23363  isoeugenol monooxygenase
Reference
1  [PMID:10702906]
  Authors
Shimoni E, Ravid U, Shoham Y
  Title
Isolation of a Bacillus sp. capable of transforming isoeugenol to vanillin.
  Journal
J Biotechnol 78:1-9 (2000)
Reference
2  [PMID:16944125]
  Authors
Yamada M, Okada Y, Yoshida T, Nagasawa T
  Title
Biotransformation of isoeugenol to vanillin by Pseudomonas putida IE27 cells.
  Journal
Appl Microbiol Biotechnol 73:1025-30 (2007)
Reference
3  [PMID:17516050]
  Authors
Yamada M, Okada Y, Yoshida T, Nagasawa T
  Title
Purification, characterization and gene cloning of isoeugenol-degrading enzyme from Pseudomonas putida IE27.
  Journal
Arch Microbiol 187:511-7 (2007)
DOI:10.1007/s00203-007-0218-9
  Sequence
Reference
4  [PMID:20091296]
  Authors
Ryu JY, Seo J, Unno T, Ahn JH, Yan T, Sadowsky MJ, Hur HG
  Title
Isoeugenol monooxygenase and its putative regulatory gene are located in the eugenol metabolic gene cluster in Pseudomonas nitroreducens Jin1.
  Journal
Arch Microbiol 192:201-9 (2010)
DOI:10.1007/s00203-010-0547-y
  Sequence
Reference
5  [PMID:23391906]
  Authors
Ryu JY, Seo J, Park S, Ahn JH, Chong Y, Sadowsky MJ, Hur HG
  Title
Characterization of an isoeugenol monooxygenase (Iem) from Pseudomonas nitroreducens Jin1 that transforms isoeugenol to vanillin.
  Journal
Biosci Biotechnol Biochem 77:289-94 (2013)
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.88
IUBMB Enzyme Nomenclature: 1.13.11.88
ExPASy - ENZYME nomenclature database: 1.13.11.88
BRENDA, the Enzyme Database: 1.13.11.88

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