KEGG ENZYME: 1.13.11.9

ENZYME: 1.13.11.9

Entry

EC 1.13.11.9                Enzyme

Name

2,5-dihydroxypyridine 5,6-dioxygenase;
2,5-dihydroxypyridine oxygenase;
pyridine-2,5-diol dioxygenase;
NicX

Class

Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen

Sysname

2,5-dihydroxypyridine:oxygen 5,6-oxidoreductase

Reaction(IUBMB)

2,5-dihydroxypyridine + O2 = N-formylmaleamic acid [RN:R09125]

Reaction(KEGG)

R09125

Substrate

2,5-dihydroxypyridine [CPD:C01059];
O2 [CPD:C00007]

Product

N-formylmaleamic acid [CPD:C18232]

Comment

Requires Fe2+.

History

EC 1.13.11.9 created 1965 as EC 1.13.1.9, transferred 1972 to EC 1.13.11.9, modified 2010

Pathway

ec00760

Nicotinate and nicotinamide metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K18028

2,5-dihydroxypyridine 5,6-dioxygenase

Genes

SMAR:

SM39_1771

SMAC:

SMDB11_1584

SMW:	SMWW4_v1c23260

SPE:

Spro_2312

SRR:	SerAS9_2269

SRL:	SOD_c21420(nicX)

SRY:	M621_11735

SPLY:

Q5A_011870(nicX)

SRS:	SerAS12_2269

SRA:	SerAS13_2270

SLQ:	M495_11505

SERF:

L085_17115

SQU:	E4343_04630

SFJ:	SAMEA4384070_2381

SOF:	NCTC11214_04488

SURI:

J0X03_12365

SNEM:

NLX84_11780

SNEV:

OI978_19650

SGRI:

SGBXF1_02348(nicX)

SSAR:

SSARUM_002235

GQU:	AWC35_02975

PAO:	Pat9b_5175

PPHO:

CTZ24_20570

PDIP:

KFZ74_23395

TCI:	A7K98_12095

TPTY:

NCTC11468_02202

PMAK:

PMPD1_1918

PPU:	PP_3945(nicX)

PPF:

Pput_1892

PPG:	PputGB1_3580

PPW:	PputW619_2138

PPT:

PPS_4058

PPB:	PPUBIRD1_1863

PPI:

YSA_08963

PPX:

T1E_5122

PPUT:

L483_21715

PPUN:

PP4_17770

PALD:

LU682_010740

PFS:

PFLU_2800

PFB:

VO64_0127

PAZO:

AYR47_21620

PMED:

E3Z27_20705

PTOL:

I7845_13265

PMAB:

U0037_14005

PFW:	PF1751_v1c24710

PFF:	PFLUOLIPICF724375

PFX:	A7318_11825

PLUL:

FOB45_03920

PSK:	U771_14860

PFZ:	AV641_08730

PANR:

A7J50_2717

PSIL:

PMA3_12570

PYM:	AK972_2676

PGG:	FX982_03129

PVK:	EPZ47_21845

PEZ:	HWQ56_12835

PWY:	HU734_022660

PTAE:

NCTC10697_01684

PPEG:

KUA23_13470

PNB:	NK667_12115

PTAI:

ICN73_23205

PPAO:

K3169_11260

PSJY:

AA098_03900 AA098_17790 AA098_21325

PSNC:

C0058_17205

PFOU:

OZ911_18915

PKV:	QYQ93_13725

PBAM:

NUH87_25765

PCAA:

V6L78_20130

PSML:

V6P94_12490

PSIV:

LZ023_19815

AWU:	BEN71_18360

AGU:

AS4_40080

HAM:

HALO0548

HSV:	HNO53_05730

HPRO:

LMS44_20650

HASO:

B2G49_16060

HAMS:

NF683_09270

HAAH:

HALA3H3_470126(nicX)

HABD:

OM794_20105

HAFN:

KF947_08770

HAMC:

HNO52_05080

HVN:	EI420_02005

HOL:	HORIV_64960

HNP:	SR894_18970

HTT:	HZS52_07580

HAXI:

HAALTHF_14370n

HTX:	EKK97_06015

ADI:

B5T_02297

MARS:

A8C75_16295

MRZ:	KDW95_18750

RPJ:	N234_04330 N234_15835 N234_24890

REH:	H16_A0925(h16_A0925) H16_A2810(h16_A2810) H16_B0813(h16_B0813)

CNC:	CNE_1c08750 CNE_1c27100 CNE_2c07610

CUH:	BJN34_04910 BJN34_15135 BJN34_29420

REU:	Reut_A2512 Reut_B3788

CTI:	RALTA_A0870 RALTA_A2299

CBW:	RR42_m3126 RR42_s0171 RR42_s1172

CGD:	CR3_0579(ampS) CR3_0803(ampS) CR3_3007(nicX)

CCUP:

BKK81_07370 BKK81_15650

CUP:	BKK80_06670 BKK80_16605

CUU:	BKK79_05480 BKK79_16350

CPAU:

EHF44_27015

COX:	E0W60_08960 E0W60_14770 E0W60_23265

CCAM:

M5D45_06905

CCAX:

KZ686_07225 KZ686_20175

CUK:	KB879_04570 KB879_08785 KB879_26000

CNAN:

A2G96_05375 A2G96_17625

CUPR:

IC580_03080 IC580_04195 IC580_15500

CUPP:

CTP10_R08100 CTP10_R24680 CTP10_R40320

BSAV:

WS86_30280

BCM:	Bcenmc03_3486

BCH:	Bcen2424_4039

BCJ:

BCAM1142

BCEN:

DM39_3879

BCEO:

I35_4989

BMJ:	BMULJ_03960

BMU:

Bmul_4550

BMK:

DM80_3720

BMUL:

NP80_4069

BCED:

DM42_3948

BCON:

NL30_06920

BSEM:

WJ12_25610

BPSL:

WS57_08415

BMEC:

WJ16_24240

BSTG:

WT74_20825

BANN:

JFN94_22955 JFN94_25175

BARI:

NLX30_32985 NLX30_38905

BAEN:

L3V59_37165

BGD:	bgla_1g12670

BGO:

BM43_2599

BYI:	BYI23_D006280 BYI23_D011200

BGP:	BGL_1c11600

BPLA:

bpln_1g11120

BUM:	AXG89_36965

BUI:	AX768_15625

BURK:

DM992_20105

BURT:

BTHE68_44290 BTHE68_70100

BXE:

Bxe_C0216

BXB:

DR64_8215

BFN:

OI25_6799

BCAI:

K788_0007381

PTER:

C2L65_30585

PTRO:

G5S35_31885

PPAI:

E1956_29580

PLAD:

PPGU16_60200

PAZZ:

PBP221_62650

PPK:	U875_06205

PPNO:

DA70_23280

PPNM:

LV28_07430

PRB:	X636_03370

PPUL:

RO07_01615

PSPU:

NA29_10040 NA29_11640 NA29_13055

PAPI:

SG18_01755

PVE:	UC34_12640 UC34_17850

POX:	MB84_21140 MB84_22640

PTX:	ABW99_20545

PFG:	AB870_04880

PNR:	AT302_14240 AT302_20395

PAND:

DRB87_16370

PFIB:

PI93_010200

PCOM:

NTU39_19585

PANN:

PanNE5_26970

PANX:

OYT13_17860

CABA:

SBC2_37490(nicX_1) SBC2_53530(nicX_2)

BUO:	BRPE64_DCDS05940 BRPE64_DCDS10990

CABK:

NK8_47380 NK8_58470

BUE:	BRPE67_DCDS08100 BRPE67_DCDS13330

BPE:	BP0581 BP1957

BPC:	BPTD_0590 BPTD_1929

BPER:

BN118_0957 BN118_3486

BPET:

B1917_3473

BPEU:

Q425_15030 Q425_35680

BPAR:

BN117_0269 BN117_1484

BPA:	BPP0271 BPP2330

BBH:	BN112_1734 BN112_3143

BBR:	BB0274 BB1781

BBM:	BN115_0259 BN115_1683

BBX:	BBS798_0269 BBS798_1682

BAV:	BAV1529 BAV2742

BBRO:

BAU06_10300 BAU06_12295 BAU06_25035

BFZ:	BAU07_14190 BAU07_23450 BAU07_25070

BPDZ:

BBN53_17720

BOH:	AKI39_11515

BGM:	CAL15_11470 CAL15_12490

BGV:	CAL12_01120 CAL12_10370 CAL12_12830

AXY:	AXYL_02466

AXO:	NH44784_051911

AXN:	AX27061_3327

AXX:	ERS451415_03307

ADT:	APT56_16400

AIS:	BUW96_02610 BUW96_28750

ASW:	CVS48_09645

ACHR:

C2U31_23370

ACHB:

DVB37_25110

APES:

FOC84_00570

AAEG:

RA224_20485

ACHA:

KYT87_26345

ACHH:

ABFG95_01695

PUD:	G9Q38_03255 G9Q38_04180 G9Q38_04670

AKA:	TKWG_00615 TKWG_01105 TKWG_19790

AMIM:

MIM_c02050 MIM_c31080

AFA:	UZ73_03775 UZ73_10785 UZ73_14565 UZ73_14765

AFQ:	AFA_13575 AFA_14965(nicX) AFA_15135(picD) AFA_18565(hpaX)

AAQU:

D3M96_14525 D3M96_15895 D3M96_16075

AAMM:

FE795_12525 FE795_13825 FE795_14000

ALCN:

MID00_11700 MID00_11895 MID00_13855

PHN:	PAEH1_03580

ODI:

ODI_R3898

PIG:	EGT29_00800 EGT29_02745 EGT29_03390

PACR:

FXN63_06615

NARM:

N7E01_07645 N7E01_09560 N7E01_09865

RHF:	EUB48_20920

ACK:	C380_17105

ASAH:

AVXHC19_39810

AAV:

Aave_3930

AAA:

Acav_3827

DAC:

Daci_1850

DEL:	DelCs14_4788

DTS:	BI380_15400

DHK:	BO996_08760

DLA:	I6G47_15030

VAP:	Vapar_4293

VAM:	C4F17_28025

VAE:	RZE79_30780 RZE79_31710

CTES:

O987_23190

CRJ:	QMY55_00795

LIM:	L103DPR2_00582(nicX)

LIH:	L63ED372_00060(nicX)

HYB:

Q5W_22175

HYL:	LPB072_00950

HYC:	E5678_16815

HPSE:

HPF_00905(nicX)

HYN:	F9K07_01290 F9K07_02925 F9K07_21765

HTN:	KI616_00800

HCZ:	G9Q37_17660

HYY:	JNX00_00865

HYDR:

SOM08_00745

HYDA:

ABSY19_00105 ABSY19_13570

HSE:	Hsero_1325

HSZ:	ACP92_06630

HEE:	hmeg3_19265

HFR:	G5S34_06840

MTIM:

DIR46_21155

XYK:	GT347_17965

XYG:	R9X41_15970

AZR:	CJ010_02410 CJ010_22080

MLN:	A9174_33965

MAMO:

A6B35_09700

MESP:

C1M53_21410

MESR:

FGU64_17615

AAK:	AA2016_6001 AA2016_6584

SIX:	BSY16_4976

ATA:	AWN88_01320

RLG:

Rleg_6046

RTR:	RTCIAT899_PC08580

RAD:	CO657_35880

RII:	FFM53_028515

RRG:	J3P73_36855

RAW:	NE851_01760

RSUL:

N2599_21415

RRHO:

PR018_21730

RTU:	PR017_19735

RBW:	RLCC275e_26400

SHZ:

shn_30285

SSUM:

Q9314_24280

HOE:	IMCC20628_03533

OCC:	KMS41_20740

OCH:	CES85_3503(nicX)

BJA:	bll6088(bll6088)

BJU:	BJ6T_36210

BJP:	RN69_17555

BRA:

BRADO2312

BRS:

S23_21870

AOL:

S58_52840

BRC:	BCCGELA001_25855

BRAD:

BF49_5545

BRO:	BRAD285_5030

BRK:	CWS35_28865

BOT:	CIT37_06750 CIT37_23840

BRQ:	CIT40_22420

BGQ:	X265_12340 X265_13550

BGZ:	XH91_11115

BSYM:

CIT39_21615

BBET:

F8237_26010

BARH:

WN72_34005 WN72_44280

BVZ:	BRAD3257_3041

BEL:	BE61_17030

BDG:	LPJ38_32260

BQB:	J4P68_0007725

BSEI:

KMZ68_19940

BBAN:

J4G43_036475

BCAN:

BcanWSM471_26940

BBRA:

QA636_11880

BGK:	IC762_04975

BCOU:

IC761_11300

BPAH:

QA639_37430

BXN:	I3J27_26420

BRAZ:

LRP30_27995

BOY:	LQG66_12750

BRAY:

ML401_24365

RBK:	E0H22_02850 E0H22_16390

RHOR:

ONR75_09780

RHOL:

RBJ75_17330 RBJ75_19365

TRB:	HB776_11430

TALZ:

RPMA_17200

TARD:

RSO67_05265 RSO67_25815

PTAW:

DW352_16490

HMC:	HYPMC_0203

RHZ:	RHPLAN_69100

PHL:

KKY_3788

PLEO:

OHA_1_03169(nicX)

HDI:	HDIA_0234(nicX)

AUB:	LXB15_07185

PSIN:

CAK95_12375 CAK95_14180 CAK95_27070

PPHR:

APZ00_25430

SIW:	GH266_12020

OAR:	OA238_c08170

CID:

P73_3892

CEH:	CEW89_08860

CON:	TQ29_12570 TQ29_12770 TQ29_16070

PABY:

Ga0080574_TMP31

SAGU:

CDO87_22440

MALU:

KU6B_06560

ACLD:

ACIRQ6_10870

FYT:	QF092_07150

PYE:	A6J80_00020 A6J80_23200

PSEW:

JHW44_20155

PFIS:

JHX87_02950

DAA:	AKL17_3241

LVS:	LOKVESSMR4R_03232(nicX)

PALW:

PSAL_024210(nicX)

FAP:	GR316_10985

GCE:	KYE46_03215

NSM:	JO391_01895

SPJJ:

V6U71_03575(ndpE)

SNJ:	A7E77_04545

SMY:	BJP26_14900

SNAP:

PQ455_05085

SBAR:

H5V43_21650(ndpE)

SLAA:

EUU25_06890(ndpE)

ELQ:	Ga0102493_111595

NTD:	EGO55_05275

GOY:	GLS_c14230

GAL:	A0U94_10315

GTI:	FXF46_02575

GSP:	IGS75_12410

GXL:

H845_1042

KEU:	S101446_01672(nicX)

KSC:	CD178_02878(nicX)

KRE:	GWK63_12515

APT:	APA01_23760

APW:	APA42C_23760

APF:	APA03_23760

APU:	APA07_23760

APG:	APA12_23760

APQ:	APA22_23760

APX:	APA26_23760

APZ:	APA32_23760

APK:	APA386B_1210

ASV:	WG31_11905

APOM:

CPF11_08775

ATO:	CIW82_09425

AASC:

A4S02_13830

ACET:

DS739_11635

KHA:	IFJ82_11095

NGU:	QN315_14015

ROS:	CTJ15_00290

RMUC:

FOB66_01435

SRF:	LHU95_20255

ALI:	AZOLI_p20433

ABS:	AZOBR_p480011

ABQ:	ABAZ39_30180

ABF:	AMK58_25905

ATI:	AL072_30665

AHU:	A6A40_15010

AZT:	TSH58p_19530

AZM:	DM194_20100 DM194_21870

AOZ:	HUE56_08300

AARE:

D3093_29340

AZS:	E6C72_22660

AZP:	E6C67_13215

HTQ:	FRZ44_18690

HADH:

FRZ61_39250 FRZ61_46310

APB:	SAR116_0287

BACA:

FAY30_11695

PARH:

I5S86_12070

MYW:	BVC93_06675 BVC93_12850

MVQ:

MYVA_3797

ROA:	Pd630_LPD07329

RCR:	NCTC10994_03415

RFA:	A3L23_03312(nicX)

RHS:	A3Q41_00020(nicX)

SVL:	Strvi_4768

SRC:	M271_44585

SMAL:

SMALA_0613

SSOI:

I1A49_06645

STRE:

GZL_07763

SNR:	SNOUR_35470

SALU:

DC74_1449

SALL:

SAZ_07780

SAUO:

BV401_06895

SBAT:

G4Z16_06625

SINE:

KI385_04355

SDUR:

M4V62_06910

SLON:

LGI35_43460

SANT:

QR300_41505

SYUN:

MOV08_36035

SPRF:

K4G22_30140

JME:	EEW87_000025

SAIU:

J4H86_13115

FAL:

FRAAL6169

AOI:

AORI_4776

AJA:	AJAP_15760(nicX)

AMQ:	AMETH_3951

AMYC:

CU254_15755

AMYY:

YIM_29130(nicX)

AACD:

LWP59_17615

AROO:

NQK81_43040

ATHM:

L1857_21475

ACOR:

LCL61_20970

AMYE:

MJQ72_22195

AMYS:

CVV72_04600

PSEA:

WY02_22100

PSEE:

FRP1_20590

PSEH:

XF36_05315

PSEQ:

AD006_28010

PECQ:

AD017_07605

PHH:	AFB00_17850

PAUT:

Pdca_13590

PBRO:

HOP40_05480

PSDS:

K1T35_32445

ACTY:

OG774_13255

PSUU:

Psuf_015390

SBAE:

DSM104329_02297(nicX_1) DSM104329_02308(nicX_2) DSM104329_02367(nicX_3) DSM104329_02480(nicX_4) DSM104329_03697(nicX_5) DSM104329_03708(nicX_6)

HAAD:

MW046_15080

HLS:	KU306_03805

SRUB:

C2R22_21800

MJN:	MjAS7_2157

» show all

Reference

1 [PMID:13475371]

Authors

BEHRMAN EJ, STANIER RY.

Title

The bacterial oxidation of nicotinic acid.

Journal

J Biol Chem 228:923-45 (1957)

Reference

2 [PMID:5578917]

Authors

Gauthier JJ, Rittenberg SC

Title

The metabolism of nicotinic acid. I. Purification and properties of 2,5-dihydroxypyridine oxygenase from Pseudomonas putida N-9.

Journal

J Biol Chem 246:3737-42 (1971)

Sequence

[ppu:PP_3945]

Reference

3 [PMID:5578918]

Authors

Gauthier JJ, Rittenberg SC.

Title

The metabolism of nicotinic acid. II. 2,5-dihydroxypyridine oxidation, product formation, and oxygen 18 incorporation.

Journal

J Biol Chem 246:3743-8 (1971)

Sequence

[ppu:PP_3945]

Reference

4 [PMID:18678916]

Authors

Jimenez JI, Canales A, Jimenez-Barbero J, Ginalski K, Rychlewski L, Garcia JL, Diaz E

Title

Deciphering the genetic determinants for aerobic nicotinic acid degradation: the nic cluster from Pseudomonas putida KT2440.

Journal

Proc Natl Acad Sci U S A 105:11329-34 (2008)
DOI:10.1073/pnas.0802273105

Sequence

[ppu:PP_3945]

Other DBs

ExplorEnz - The Enzyme Database:

1.13.11.9

IUBMB Enzyme Nomenclature:

1.13.11.9

ExPASy - ENZYME nomenclature database:

1.13.11.9

UM-BBD (Biocatalysis/Biodegradation Database):

1.13.11.9

BRENDA, the Enzyme Database:

1.13.11.9

CAS:

9029-57-6

DBGET integrated database retrieval system