KEGG   ENZYME: 1.13.11.91
Entry
EC 1.13.11.91               Enzyme                                 
Name
3-mercaptopropionate dioxygenase;
mdo (gene name);
3-mercaptopropionic acid dioxygenase;
3-sulfanylpropanoate dioxygenase
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
Sysname
3-sulfanylpropanoate:oxygen oxidoreductase
Reaction(IUBMB)
3-sulfanylpropanoate + O2 = 3-sulfinopropanoate [RN:R12662]
Reaction(KEGG)
R12662
Substrate
3-sulfanylpropanoate [CPD:C22312];
O2 [CPD:C00007]
Product
3-sulfinopropanoate [CPD:C22313]
Comment
This bacterial enzyme contains an iron(2+) atom coordinated by three protein-derived histidines and a Ser-His-Tyr motif. It is similar to EC 1.13.11.20, cysteine dioxygenase, and can act on L-cysteine, but has a much higher activity with its native substrate, 3-sulfanylpropanoate.
History
EC 1.13.11.91 created 2020
Orthology
K24700  3-mercaptopropionate dioxygenase
Genes
KPNKPN_02113
KPUKP1_3207
KPMKPHS_30950
KPPA79E_2131
KPHKPNIH24_12975
KPZKPNIH27_14820
KPVKPNIH29_15480
KPWKPNIH30_15760
KPYKPNIH31_14615
KPGKPNIH32_15655
KPCKPNIH10_15280
KPQKPR0928_15255
KPTVK055_0348
KPOKPN2242_13520
KPRKPR_2657
KPJN559_2177
KPID364_10830
KPAKPNJ1_02349
KPSKPNJ2_02309
KPXPMK1_04477
KPBFH42_06660
KPNEKU54_010860
KPNULI86_10815
KPNKBN49_3218
KVAKvar_2162
KPEKPK_2210
KPKA593_25620
KVDKR75_23540
KVQSP68_04195
KOXKOX_22645
KOEA225_3406
KOYJ415_15000
KOMHR38_20940
KOKKONIH1_16680
KOCAB185_19225
KQUAVR78_19645
EAEEAE_17230
EARCCG30445
KQVB8P98_11835
KLLBJF97_16800
KLWDA718_12135
KARLGL98_10350
KGRJJJ10_12105
RORRORB6_04320
RONTE10_17160
RPLNB1209_10975
RAODSD31_10750
RTGNCTC13098_03110
IZHFEM41_08875
EBUCUC76_00425
SMAFD781_3811
SRZAXX16_1272
SERMCLM71_15115
SFJSAMEA4384070_2031
SOFNCTC11214_03693
SENPKHA73_10080
RAHRahaq_2235
RACEJHW33_05650
RAQRahaq2_2339
RAAQ7S_11305
RVCJ9880_18405
GQUAWC35_21290
EAMEGXP68_10730
BNGEH206_03165
SODSant_P0280
EBIEbC_32610
PAOPat9b_2825
KLNLH22_08480
PANSFCN45_13670
TCIA7K98_11630
SMLSmlt0074
SMTSmal_0030
BUJBurJV3_0029
SMZSMD_0027
SACZAOT14_00260
STEKAXG53_17740
SRHBAY15_0158
SLMBIZ42_09715
STENCCR98_00135
STEMCLM74_00175
STESMG068_00135
SINCDAIF1_00320
SPAQSTNY_R00340
PSUPsesu_1262
PSUWWQ53_01035
PMEXH4W19_06410
PJPLAG73_05340
PDDMNQ95_00015
LABLA76x_2383
LAQGLA29479_2751
LCPLC55x_2406
LGULG3211_2831
LEZGLE_2837
LEMLEN_2194
LMBC9I47_1407
LYTDWG18_05335
LUEDCD74_03595
LYJFKV23_09380
LSOLGOY17_07420
LSXH8B22_01125
LHXLYSHEL_30870
LCASLYSCAS_30840
LCICINQ41_06220
LAVIINQ42_05995
LUMCNR27_11695
LUSE5843_06375
LUGFPZ22_13005
THESFHQ07_01680
THEHG7079_08425
TCNH9L16_11655
TBVH9L17_01765
XBCELE36_12710
RHDR2APBS1_2402
RGLCS053_08860
RTHLRK53_12755
DJICH75_05010
DJAHY57_10575
DTXATSB10_07260
DYEEO087_08025
DCSISN74_06470
DTLH8F01_17200
DKOI596_2160
LRZBJI69_16600
LPYFIV34_08800
XBAC7S18_11570
RBDALSL_1933
VNAPN96_21280
VEJVEJY3_20461
PAEPA2602
PAEVN297_2681
PAEIN296_2681
PAUPA14_30440
PAGPLES_25681
PAFPAM18_2373
PNCNCGM2_3617
PAEBNCGM1900_3913
PDKPADK2_11650
PSGG655_11990
PRPM062_13825
PAEPPA1S_12600
PAERPA1R_gp0402
PAEMU769_12165
PAELT223_13125
PAESSCV20265_2652
PAEUBN889_02867
PAEGAI22_21310
PAECM802_2678
PAEOM801_2547
PREPCA10_18560
PCQPcP3B5_55350
PMUIG4G71_06785 G4G71_13885
PPUPP_3216
PPGPputGB1_2513
PPUTL483_13520
PPUNPP4_28340
PORAPT59_09275
PMOLCLJ08_25500
PSTPSPTO_1793
PSBPsyr_3601
PSYRN018_07420
PSPPSPPH_3612
PSAVPSA3335_21955
PAMGBKM19_021500
PCIPCH70_36900
PAVLBKM03_20740
PVDCFBP1590__1674
PCABJGS08_06810
PFLPFL_1375
PPRCPFLCHA0_c14110
PPROPPC_1429
PFSPFLU_3685
PFCPflA506_2354
PFBVO64_5651
PTVAA957_26810
PVRPverR02_12895
PAZOAYR47_19385
POIBOP93_10570
PFWPF1751_v1c31280
PFFPFLUOLIPICF720575
PFXA7318_15220
PSJPSJM300_08630
PSVPVLB_11810
PSKU771_13185
PCHEY04_15430
PCZPCL1606_25550
PRHLT40_05185
PSWLK03_17315
PPVNJ69_07865
PSESPSCI_0202
PSEMTO66_18620
PSECCCOS191_2655
PSOSPOS17_1395
PANRA7J50_2281
PYMAK972_3381
POJPtoMrB4_28320
PGGFX982_00057
PEZHWQ56_13340
PBZGN234_29645
PSEPC4K39_1419
PVWHU752_007980
PTRTHU722_0012540
PXAKSS93_20010
PQIKH389_11370
PPRGHU725_010140
PASGKSS96_12975
PFAKKSS94_09970
PMAMKSS90_12710
PTWTUM18999_21930 TUM18999_30890
PWYHU734_012725
AVNAvin_30970
AVLAvCA_30970
AVDAvCA6_30970
ACXAchr_17290
ACDAOLE_08655
MBAHHYN46_06875
SLOShew_3576
SMAVCFF01_17745
PXIJ5O05_20990
TLOJ9253_15235
THIPN838_10350
WMAWM2015_1273
WOCBA177_06005
HAAA5892_12020
MARSA8C75_17040
NJPNEJAP_2135
ENMEBS_1100
RPIRpic_4463
RPFRpic12D_4597
RPJN234_30900
RMNTK49_16850
RINACS15_4473
REHH16_B1863(cdo)
CNCCNE_2c18080(cdo)
CUHBJN34_31885
REUReut_B5045
RMERmet_2216
CTIRALTA_B1562
CBWRR42_s2963
CGDCR3_3061
CCUPBKK81_24180
CUPBKK80_32155
CUUBKK79_23090
CPAUEHF44_11665
COXE0W60_02810
BTDBTI_5524
BOKDM82_4798
BOCBG90_5795
BSAVWS86_22210
BVIBcep1808_4826
BVEAK36_4092
BURBcep18194_B1752
BCNBcen_4107
BCHBcen2424_4259
BCMBcenmc03_3258
BCJBCAM1394
BCENDM39_3611
BCEWDM40_4282
BCEOI35_5246
BAMBamb_3683
BACBamMC406_4156
BMJBMULJ_04175
BMUBmul_4331
BMKDM80_3510
BMULNP80_4287
BCTGEM_4361
BCEDDM42_3693
BCEPAPZ15_17685
BDLAK34_4116
BPYRABD05_29225
BCONNL30_05690
BUBBW23_5071
BDFWI26_19960
BLATWK25_23780
BTEIWS51_04650
BSEMWJ12_26770
BPSLWS57_07115
BMECWJ16_25360
BSTGWT74_25735
BSTLBBJ41_20025
BANNJFN94_24055
BGLbglu_2g17800
BGUKS03_4150
BUGBC1001_1273
BGFBC1003_2169
BGDbgla_2g23150
BGOBM43_5987
BUKMYA_4394
BULBW21_5205
BUQAC233_06160
BGPBGL_2c23790
BPLAbpln_2g24090
BUDAQ610_27190
BURKDM992_18975
BXEBxe_B1914
BXBDR64_7223
BPHBphy_5233
BGEBC1002_5842
BPXBUPH_01812 BUPH_08268
BPYBphyt_1548
BFNOI25_174
BCAIK788_0006152
PSPWBJG93_28360
PHSC2L64_37875
PTERC2L65_33780
PGPCUJ91_06655
PCJCUJ87_10065
PTSCUJ90_11730
PCAFDSC91_006644
PMEGFNZ07_17025
PTROG5S35_27170
PGISI6I06_05980
PACSFAZ98_16250
PNUPnuc_1341
PNEPnec_0608
PDQCL55_00012540
POHDPM16_06380
PPKU875_06390
PPNODA70_23460
PPNMLV28_07250
PRBX636_03185
PPULRO07_01360 RO07_24385
PSPUNA29_10275
PAPISG18_01525
PVEUC34_17625
POXMB84_22400
PTXABW99_02520
PFGAB870_04655
PNRAT302_14470 AT302_16235
PANDDRB87_16185
PFIBPI93_010425
PLGNCTC10937_03895
CABASBC2_66470
BPEBP3320
BPCBPTD_3277
BPERBN118_0480
BPETB1917_0503
BPARBN117_4175
BBHBN112_3848
BBRBB4575
BBMBN115_4251
BBXBBS798_4351
BPTBpet3443
BHOD560_1649
BHMD558_1637
BHZACR54_04606
BTRMSAMEA390648702964 SAMEA390648703926
BBROBAU06_14170
BFZBAU07_14265
BPDZBBN53_00130
BOHAKI39_13480
BGMCAL15_17295
AXYAXYL_04523
AXONH44784_061301
AXNAX27061_4377
AXXERS451415_04394
ADTAPT56_20115
AISBUW96_11845
ASWCVS48_23990
ACHRC2U31_26255 C2U31_30155
ACHBDVB37_18380
ADYHLG70_01580
PUTPT7_2409
AKATKWG_22195
AMIMMIM_c31400(mdo) MIM_c36110(cdo)
AFAUZ73_14325
AFQAFA_15275
AAQUD3M96_16215
AAMMFE795_14165
ODIODI_R4373 ODI_R4379
BOZDBV39_18855
PIGEGT29_12880
PACRFXN63_15650
PARKLSG25_16080
RHYRD110_24255
POLBpro_1656
ACIOEAG14_04030
AANTHUK68_13480
VEIVeis_3618
VAPVapar_3875
VPEVarpa_4479
VPDVAPA_1c39920
VBOCKY39_09260
VAMC4F17_30945
CSERCCO03_16080
OTKC6570_08850
HYNF9K07_00930
DIHG7047_23105
DAERH9K75_13545
DRGH9K76_15470 H9K76_20680
MPTMpe_A0862
METPC1M51_13355
HARHEAR1183
JAGGJA_1725
HHTF506_03470
HEEhmeg3_03390
HHFE2K99_19065
HFRG5S34_20395
CFUCFU_2069
CARELT85_2611
CPRACPter91_2702
MASWAM586_19690
MASSCR152_17940
MALIEYF70_13805
MUMFCL38_05980
MANCIV454_30430
GLCJQN73_06395
RGERGE_41750
MIUABE85_04995
RGUA4W93_07405
XYKGT347_24205
ATERMW290_09545
URUDSM104443_03822
UPLDSM104440_03385
OTROTERR_21070
ABREpbN1_34560
ATWC0099_15030
AOAdqs_2515
AZRCJ010_11130
THKCCZ27_12840
BPRCD521_1134
BEBABWI17_07895
RLNJ0663_18650
BRABRADO1837
BBTBBta_2159
BRSS23_10840
AOLS58_57150
BROBRAD285_5450
BGQX265_39690
BGZXH91_34245
BBETF8237_20295
OCAOCAR_6784
OCGOCA5_c12920
OCOOCA4_c12920
XAUXaut_1493
XDIEZH22_25005
BIDBind_3544
MSLMsil_2674
MSCBN69_2603
MBRYB1812_07810
MROSEHO51_16590
MHEYH2LOC_003215
MPARF7D14_06900
MTWCQW49_08975
CAKCaul_2664
CSECseg_2033
CFHC1707_21710
NREBES08_00330
NOVTQ38_014280
SGISGRAN_0452
SPHOC3E99_01795
SWISwit_1712
SPHDHY78_17025
SPANAWL63_15885
SECHB18_25445
SJPSJA_C2-04310
SCHSphch_3545
SYBTZ53_08940
SPHTK426_24859
SYAA6768_10345
SPYGYGS_C1P1307
SUFLFIL70_19075
SBARH5V43_20725
SAMISAMIE_1002950 SAMIE_1016930
GOHB932_2957
GALA0U94_05750
GTIFXF46_12775
GFAMKW11_13235
AACEA0U92_03210
ACEKFLP30_11025
SRFLHU95_07550
AZLAZL_b02610
ABSAZOBR_p330112
ABQABAZ39_27365
AZTTSH58p_30400
AZMDM194_18955
AZZDEW08_24195
NAOY958_18715
HTQFRZ44_26450
HADHFRZ61_20910
BSTGYO_2891
BHTDIC78_18340
BMOJHC660_24720
BANBA_2633
BARGBAA_2633
BATBAS2453
BAHBAMEG_1959
BAIBAA_2699
BAXH9401_2508
BANTA16_26700
BANRA16R_27050
BANSBAPAT_2529
BANHHYU01_13080
BANVDJ46_1416
BCEBC2617
BCABCE_2660
BCZBCE33L2378
BCRBCAH187_A2701
BCBBCB4264_A2657
BCUBCAH820_2651
BCGBCG9842_B2667
BCQBCQ_2499
BCXBCA_2724
BALBACI_c26080
BNCBCN_2514
BCERBCK_21680
BCEFBcrFT9_02074
BCYBcer98_1882
BTKBT9727_2415
BTLBALH_2369
BTBBMB171_C2371
BTTHD73_3375
BTHRYBT1520_16640
BTHIBTK_16655
BTCCT43_CH2616
BTFYBT020_13215
BTMMC28_1836
BTGBTB_c27430
BTIBTG_06595
BTNBTF1_10365
BTHTH175_ch2661
BTHUYBT1518_14490
BTWBF38_3813
BTHYAQ980_14360
BWEBcerKBAB4_2507
BWWbwei_2324(cdoA)
BMYOBG05_3319
BBYCY96_11915
BWDCT694_14135
BTROFJR70_06110
BMOBMLA2C4_14055
BNTGSN03_13105
BPACLMD38_13825
BMHBMWSH_1668
VIRX953_14930
VHLBME96_14255
VILCFK37_09180
VNECFK40_00025 CFK40_12610
PBUTDTO10_07025
PNPIJ22_14000
POWIJ21_32750
PPSCEHS13_09330
PCELHUB94_04505
COHNKCTCHS21_05330
AACOK1I37_00635
NBRO3I_034070
NNONONO_c67080
NOZDMB37_12830
NAHF5544_09310
NIEKV110_14335
RAVAAT18_12070
RRZCS378_01840
SVLStrvi_4009 Strvi_8295
SHYSHJG_0437
SHOSHJGH_0271
SRCM271_30150
SMALSMALA_3006
SSOII1A49_18945
SCTSCAT_p1038
SCYSCATT_p06980
SBHSBI_06641(cdo)
STRMM444_17355
SPRISPRI_1136
SALWCP975_10895
SGMGCM10017557_33670
SCADDN051_37380
SAUOBV401_18685
SSIAA7J05_24795
SALFSMD44_06086
SALJSMD11_4272
SLXSLAV_18760
SFKKY5_2501
SLKSLUN_33285
SGDELQ87_00520
SASTCD934_10040
STIRDDW44_26540
SKACP970_29970
SGZC0216_30030
SVNCP980_17405
SRKFGW37_12130
SFICEIZ62_19295
SSPODDQ41_27810
SVRCP971_05040
SBYH7H31_22785
SSUBCP968_17450
SPACB1H29_09170
SBATG4Z16_21990
SSPBCP982_29210 CP982_37260
SNFJYK04_04301
SMOBJ7W19_11915
SPEUCGZ69_31255
SHKJ2N69_10765
SMAOCAG99_20055
SGOBtest1122_08015
SLFJEQ17_02615
KAUB6264_00450 B6264_25015
KITCFP65_6603
GSDM3M28_10750
NOOFE634_01170
NMESH9L09_11785
PSIMKR76_25615
KQIF1D05_31665
NGVCDO52_24645
SROSros_1508
NOABKM31_19670 BKM31_38140
NOWGBF35_07165
NCXNocox_06600
NGNLCN96_07230
SENSACE_0349
SACCEYD13_11810
AOIAORI_6200
AJAAJAP_08410
AMYBBKN51_24500
AMYYYIM_41650
AORISD37_23210 SD37_30325
PHHAFB00_20915
KPHYAOZ06_17025 AOZ06_27160
PSUUPsuf_031540
SNASnas_1987 Snas_5454
AEYCDG81_01365
RMARGBA65_18415
NPUNpun_R0623
NONNOS3756_01070
NFLCOO91_08661
NSHGXM_02350
NEDHUN01_09840
CALOCal7507_4484
DFSHGD76_01070
TOQHCG51_03625
STISthe_1545 Sthe_2451
FGIOP10G_3682
GRWFTO74_13110
HDABB347_14670
NAYHYG81_24305
 » show all
Reference
1  [PMID:19001372]
  Authors
Bruland N, Wubbeler JH, Steinbuchel A.
  Title
3-mercaptopropionate dioxygenase, a cysteine dioxygenase homologue, catalyzes the initial step of 3-mercaptopropionate catabolism in the 3,3-thiodipropionic acid-degrading bacterium variovorax paradoxus.
  Journal
J Biol Chem 284:660-72 (2009)
DOI:10.1074/jbc.M806762200
  Sequence
Reference
2  [PMID:25307852]
  Authors
Driggers CM, Hartman SJ, Karplus PA.
  Title
Structures of Arg- and Gln-type bacterial cysteine dioxygenase homologs.
  Journal
Protein Sci 24:154-61 (2015)
DOI:10.1002/pro.2587
  Sequence
Reference
3  [PMID:26624219]
  Authors
Pierce BS, Subedi BP, Sardar S, Crowell JK.
  Title
The "Gln-Type" Thiol Dioxygenase from Azotobacter vinelandii is a 3-Mercaptopropionic Acid Dioxygenase.
  Journal
Biochemistry 54:7477-90 (2015)
DOI:10.1021/acs.biochem.5b00636
Reference
4  [PMID:26272617]
  Authors
Tchesnokov EP, Fellner M, Siakkou E, Kleffmann T, Martin LW, Aloi S, Lamont IL, Wilbanks SM, Jameson GN.
  Title
The cysteine dioxygenase homologue from Pseudomonas aeruginosa is a 3-mercaptopropionate dioxygenase.
  Journal
J Biol Chem 290:24424-37 (2015)
DOI:10.1074/jbc.M114.635672
  Sequence
[pae:PA2602]
Reference
5  [PMID:26878277]
  Authors
Fellner M, Aloi S, Tchesnokov EP, Wilbanks SM, Jameson GN.
  Title
Substrate and pH-Dependent Kinetic Profile of 3-Mercaptopropionate Dioxygenase from Pseudomonas aeruginosa.
  Journal
Biochemistry 55:1362-71 (2016)
DOI:10.1021/acs.biochem.5b01203
Reference
6  [PMID:27311613]
  Authors
Crowell JK, Sardar S, Hossain MS, Foss FW Jr, Pierce BS.
  Title
Non-chemical proton-dependent steps prior to O2-activation limit Azotobacter vinelandii 3-mercaptopropionic acid dioxygenase (MDO) catalysis.
  Journal
Arch Biochem Biophys 604:86-94 (2016)
DOI:10.1016/j.abb.2016.06.009
Reference
7  [PMID:31045343]
  Authors
Aloi S, Davies CG, Karplus PA, Wilbanks SM, Jameson GNL.
  Title
Substrate Specificity in Thiol Dioxygenases.
  Journal
Biochemistry 58:2398-2407 (2019)
DOI:10.1021/acs.biochem.9b00079
  Sequence
[pae:PA2602] [reu:Reut_B5045]
Reference
8  [PMID:31750652]
  Authors
Sardar S, Weitz A, Hendrich MP, Pierce BS.
  Title
Outer-Sphere Tyrosine 159 within the 3-Mercaptopropionic Acid Dioxygenase S-H-Y Motif Gates Substrate-Coordination Denticity at the Non-Heme Iron Active Site.
  Journal
Biochemistry 58:5135-5150 (2019)
DOI:10.1021/acs.biochem.9b00674
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.91
IUBMB Enzyme Nomenclature: 1.13.11.91
ExPASy - ENZYME nomenclature database: 1.13.11.91
BRENDA, the Enzyme Database: 1.13.11.91

DBGET integrated database retrieval system