Entry |
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Name |
kanamycin B dioxygenase;
kanJ (gene name)
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
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Sysname |
kanamycin-B,2-oxoglutarate:oxygen oxidoreductase (deaminating, 2'-hydroxylating)
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Reaction(IUBMB) |
kanamycin B + 2-oxoglutarate + O2 = 2'-dehydrokanamycin A + succinate + NH3 + CO2 [RN: R10312]
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
Requires Fe2+ and ascorbate. Found in the bacterium Streptomyces kanamyceticus where it is involved in the conversion of the aminoglycoside antibiotic kanamycin B to kanamycin A.
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History |
EC 1.14.11.37 created 2013, modified 2013
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Pathway |
ec00524 | Neomycin, kanamycin and gentamicin biosynthesis |
ec01110 | Biosynthesis of secondary metabolites |
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Orthology |
K20574 | kanamycin B dioxygenase |
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Genes |
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Reference |
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Authors |
Sucipto H, Kudo F, Eguchi T |
Title |
The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the alpha-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK. |
Journal |
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Sequence |
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Other DBs |
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