KEGG   ENZYME: 1.14.11.38Help
Entry
EC 1.14.11.38               Enzyme                                 

Name
verruculogen synthase;
fmtF (gene name);
FmtOx1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
fumitremorgin B,2-oxoglutarate:oxygen oxidoreductase (verruculogen-forming)
Reaction(IUBMB)
fumitremorgin B + 2-oxoglutarate + 2 O2 + reduced acceptor = verruculogen + succinate + CO2 + H2O + acceptor [RN:R10445]
Reaction(KEGG)
Substrate
fumitremorgin B [CPD:C20630];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007];
reduced acceptor [CPD:C00030]
Product
verruculogen [CPD:C20045];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001];
acceptor [CPD:C00028]
Comment
Requires Fe2+ and ascorbate. Found in the fungus Aspergillus fumigatus. Both atoms of a dioxygen molecule are incorporated into verruculogen [1,2]. Involved in the biosynthetic pathways of several indole alkaloids such as fumitremorgin A.
History
EC 1.14.11.38 created 2013
Pathway
ec00404  Staurosporine biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K17825  verruculogen synthase
Genes
AFM: AFUA_8G00230
NFI: NFIA_045530 NFIA_093740
URE: UREG_04217 UREG_07661
Taxonomy
Reference
1  [PMID:19763315]
  Authors
Steffan N, Grundmann A, Afiyatullov S, Ruan H, Li SM
  Title
FtmOx1, a non-heme Fe(II) and alpha-ketoglutarate-dependent dioxygenase, catalyses the endoperoxide formation of verruculogen in Aspergillus fumigatus.
  Journal
Org Biomol Chem 7:4082-7 (2009)
DOI:10.1039/b908392h
  Sequence
Reference
2  [PMID:21404415]
  Authors
Kato N, Suzuki H, Takagi H, Uramoto M, Takahashi S, Osada H
  Title
Gene disruption and biochemical characterization of verruculogen synthase of Aspergillus fumigatus.
  Journal
Chembiochem 12:711-4 (2011)
DOI:10.1002/cbic.201000562
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.38
IUBMB Enzyme Nomenclature: 1.14.11.38
ExPASy - ENZYME nomenclature database: 1.14.11.38
BRENDA, the Enzyme Database: 1.14.11.38

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