KEGG   ENZYME: 1.14.11.39
Entry
EC 1.14.11.39               Enzyme                                 
Name
L-asparagine hydroxylase;
L-asparagine 3-hydroxylase;
AsnO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
L-asparagine,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
L-asparagine + 2-oxoglutarate + O2 = (2S,3S)-3-hydroxyasparagine + succinate + CO2 [RN:R10446]
Reaction(KEGG)
R10446
Substrate
L-asparagine [CPD:C00152];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(2S,3S)-3-hydroxyasparagine [CPD:C20631];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+. The enzyme is only able to hydroxylate free L-asparagine. It is not active toward D-asparagine. The beta-hydroxylated asparagine produced is incorporated at position 9 of the calcium-dependent antibiotic (CDA), an 11-residue non-ribosomally synthesized acidic lipopeptide lactone.
History
EC 1.14.11.39 created 2013
Orthology
K18058  L-asparagine oxygenase
Genes
ENC: ECL_03968
ECLG: EC036_33910
EMOR: L6Y89_16585
SMAC: SMDB11_0605 SMDB11_1104
SRR: SerAS9_0274
SPLY: Q5A_006790(asnO)
SRS: SerAS12_0274
SRA: SerAS13_0273
SFG: AV650_20270
SERA: Ser39006_015050
SERQ: CWC46_15045
SSUR: ATE40_003415
RAQ: Rahaq2_2682
ROX: BV494_24950
DSO: A4U42_21025
CED: LH89_00095 LH89_22315
DFN: CVE23_12695
DAQ: DAQ1742_04254
DDA: Dd703_1456
ETA: ETA_15700
PLU: plu3528 plu3801
PLUM: A4R40_17540 A4R40_18915
PTT: VY86_04825
XBV: XBW1_mp0174
XNE: XNC1_2155 XNC1_2460 XNC1_4045
XNM: XNC2_1351 XNC2_2084 XNC2_2366 XNC2_3898
XPO: XPG1_2874 XPG1_3240
XHO: A9255_19685
HIF: HIBPF_07710
HIL: HICON_17210
HAEG: NCTC8502_01634(asnO)
XFM: Xfasm12_0852
XFL: P303_03580 P303_04050
FAU: Fraau_1426
VTU: IX91_10335
VAQ: FIV01_12890(mppO)
VSR: Vspart_00116(asnO_1) Vspart_02171(asnO_2)
VSA: VSAL_I1305
PMAI: CF386_12490
PPG: PputGB1_5419
PPW: PputW619_0540
PPUT: L483_02555
PPUN: PP4_05200
PSP: PSPPH_4996
PSYI: MME58_00600
PFE: PSF113_0453b
PFB: VO64_2148
PSV: PVLB_22765
PPV: NJ69_17710
PSOS: POS17_3974(PputW619_0540)
PPSL: BJP27_20500
PANH: HU763_002420
PORY: EJA05_00025
PSIH: LOY51_00025
PSA: PST_3923
PSZ: PSTAB_3884(mppO)
PSR: PSTAA_4073
PSC: A458_01685
PSJ: PSJM300_01810
PSH: Psest_0326
PSTU: UIB01_01625
PSED: DM292_20880
PCHL: LLJ08_01665
PKG: LW136_01665
ASHA: G8E00_11445
MOI: MOVS_02950
MCUN: NCTC10297_00742
SHP: Sput200_2525
PRR: AT705_10755 AT705_23320
CEB: B0D95_16160
LLO: LLO_3359
MPSY: CEK71_21840
HBH: E4T21_00675
BTHG: MS2017_1703
NCI: NCTC10296_01180(asnO)
BCM: Bcenmc03_6386
BCH: Bcen2424_5877
BCJ: BCAS0290
BCEO: I35_7306
BCED: DM42_6785
BCEP: APZ15_35315
BPYR: ABD05_30850 ABD05_32945
BSEM: WJ12_20955
BMEC: WJ16_18780
BSTL: BBJ41_33110
BARI: NLX30_36345
BURK: DM992_33855
BCAI: K788_0005646
PBRY: NDK50_01175
HYF: DTO96_101676
BBRO: BAU06_22245
BFZ: BAU07_22400
BOH: AKI39_03465
KIA: G8A07_15085
JAL: BZG29_19805
HHT: F506_00300
THI: THI_1730
FKU: FGKAn22_03770
RBE: RBE_0478
RBO: A1I_02770
AMIH: CO731_01849
BIC: LMTR13_25260
KVL: KVU_PA0021
KVU: EIO_3104
CFUS: CYFUS_003163
BSIA: CWD84_18685
BWH: A9C19_04265
BACS: AUL54_07450
PARH: I5S86_03595
PFRI: L8956_04705
KEB: GXN75_09265
PABS: JIR001_15040(asnO_1)
MSA: Mycsm_07253
CPU: CPFRC_03130
CPL: Cp3995_0633(tauD)
CPG: CP316_03250
CPP: CpP54B96_0633(tauD)
CPK: CP1002_10005
CPQ: CPC231_03130
CPX: CPI19_08390
CPZ: CpPAT10_0623(tauD)
COR: Cp267_0650(tauD)
COP: CP31_03485
COD: Cp106_0606(tauD)
COS: Cp4202_0616(tauD)
COI: CPCIP5297_03275
COE: CP258_03270
COU: CP162_03155
CPSE: CPTA_01173
CPSU: CPTB_00359
CPSF: CPTC_01163
CUL: CULC22_00672(ogs)
CUC: CULC809_00665(ogs)
CUE: CULC0102_0775(ogs)
CUN: Cul210932_0700(tauD)
CUS: CulFRC11_0669(tauD)
CUQ: Cul210931_0669(tauD)
CUZ: Cul05146_0718(tauD)
CUJ: CUL131002_0678c(tauD)
CGY: CGLY_00545 CGLY_11835
CSIL: CBE74_03910
NFA: NFA_48610
NFR: ERS450000_05049(asnO)
NCY: NOCYR_4655
NBR: O3I_036890
NNO: NONO_c66170
NSL: BOX37_26935
NSR: NS506_01690(asnO)
NTP: CRH09_30780 CRH09_34590
NOZ: DMB37_15820 DMB37_36755
NOD: FOH10_01930
NAH: F5544_17945 F5544_17970 F5544_39350
NAD: NCTC11293_06239(asnO_2)
NWL: NWFMUON74_10480
NIE: KV110_36135
ROA: Pd630_LPD16019
ROZ: CBI38_01540
GOM: D7316_02856(mppO)
SRT: Srot_0774
SCO: SCO2693(SCC61A.14) SCO3236(SCE29.05c)
SALB: XNR_0986
SGB: WQO_12245
SCB: SCAB_3341 SCAB_5271
SSX: SACTE_6543
SVL: Strvi_3124
SHY: SHJG_1397
SHO: SHJGH_1231
SRC: M271_40120
SBH: SBI_00878 SBI_00941 SBI_09256
SDV: BN159_6047
SALS: SLNWT_6835
STRP: F750_6418
SFI: SFUL_5831
SCI: B446_23870
SLV: SLIV_21510(asnO1) SLIV_24200(asnO2)
SGU: SGLAU_07120
SVT: SVTN_22095
STRE: GZL_01725
SLD: T261_2127 T261_7562
SPRI: SPRI_3466
SCZ: ABE83_06835 ABE83_06955
SCX: AS200_25135
SRW: TUE45_06291(mppO_1) TUE45_06409(asnO_5) TUE45_06920(mppO_2) TUE45_pSRc_0447(vioC)
SRN: A4G23_02365(asnO_1) A4G23_04203 A4G23_04388(vioC) A4G23_05516
SCLF: BB341_15030
STSI: A4E84_04350
SKY: D0C37_06320 D0C37_30510
SALW: CP975_03210 CP975_28170
SLS: SLINC_4870
SALU: DC74_5997
SALL: SAZ_31060
SPLU: LK06_021015
SNW: BBN63_06790 BBN63_30085
SSIA: A7J05_00255 A7J05_03135 A7J05_07200 A7J05_36480
SVU: B1H20_11480
SGV: B1H19_08945 B1H19_09120 B1H19_35575
SLAU: SLA_5783
SALJ: SMD11_0981 SMD11_3232
SLX: SLAV_05480(mppO) SLAV_07260(asnO)
SFK: KY5_0486c
SGE: DWG14_08222(mppO)
SLK: SLUN_13775 SLUN_22945
SDX: C4B68_01260 C4B68_36880
SGD: ELQ87_17590
SAST: CD934_30580
SNQ: CP978_01995
STIR: DDW44_23825
SKA: CP970_40345
SGZ: C0216_32755(vioC)
SVN: CP980_28965
SNK: CP967_17245
SRK: FGW37_28385
SAQU: EJC51_46700
SCAV: CVT27_26855
SSEO: D0Z67_22420
SBY: H7H31_00850
SRIM: CP984_39215
SPHV: F9278_45090
SCIN: CP977_31230
SCHA: CP983_18395
SGX: H4W23_33125
SBAT: G4Z16_04870
SNF: JYK04_08164(asnO_2)
SPLA: CP981_21715
SATA: C5746_39315
SHUN: DWB77_00456(asnO_1)
SLIA: HA039_20700
SDW: K7C20_27875
SCAL: I6J39_11685 I6J39_22910
SFB: CP974_12965 CP974_22660 CP974_29360
SROI: IAG44_03950
SXN: IAG42_37060 IAG42_37075
SHK: J2N69_28135
STRY: EQG64_28155
SDEC: L3078_05850
SDUR: M4V62_11920
SAKB: K1J60_40335
STAA: LDH80_07070
SCT: SCAT_2689(asnO) SCAT_p1377
SCY: SCATT_26730 SCATT_p03480
KAB: B7C62_11150 B7C62_26740
KIT: CFP65_1227
STRH: GXP74_29695
MSF: IT882_06300
RTN: A6122_0640
RRY: C1O28_01960
RTE: GSU10_09185
PNV: JMY29_00220
NCA: Noca_1042
KQI: F1D05_30950
SRO: Sros_3755
NOA: BKM31_45775
FAL: FRAAL4688
KRA: Krad_3958
SEN: SACE_4278 SACE_5782
AOI: AORI_3167
AJA: AJAP_23525
AMYC: CU254_26135
AMYY: YIM_25785(asnO3)
AORI: SD37_09295 SD37_16155
AROO: NQK81_06740
APRE: CNX65_18320
SSYI: EKG83_30670
KAL: KALB_1756 KALB_2593 KALB_5896 KALB_6160
LED: BBK82_35715
ACTA: C1701_17135 C1701_25725
ALO: CRK62029
ACTU: Actkin_01205(asnO_1) Actkin_01360(asnO_2)
MAU: Micau_1827
MTEM: GCE86_18175
VMA: VAB18032_03125
MCRA: ID554_06655 ID554_16940
VER: HUT12_06305 HUT12_30735
ASE: ACPL_2187(cs1)
ACTN: L083_4621
ACTS: ACWT_2065
PLK: CIK06_09170(vioC)
PLAB: C6361_01855 C6361_01950
PLAT: C6W10_19720 C6W10_19815
PSUU: Psuf_065540(asnO)
CAI: Caci_0231 Caci_6328
RXY: Rxyl_1155
CYB: CYB_1854
LET: O77CONTIG1_00553(cs2)
THEU: HPC62_14935
GEN: GM3709_3716
GES: VT84_06800(asnO)
CPI: Cpin_2834
CHIH: GWR21_13095
NKO: Niako_2766
FLN: FLA_1331
FJO: Fjoh_3169
CIO: CEQ15_06495
CHRY: CEY12_03435
CJT: EG359_03155
CCAU: EG346_19170
CGLE: NCTC11432_00670(asnO)
 » show all
Reference
1  [PMID:17373765]
  Authors
Strieker M, Kopp F, Mahlert C, Essen LO, Marahiel MA
  Title
Mechanistic and structural basis of stereospecific Cbeta-hydroxylation in calcium-dependent antibiotic, a daptomycin-type lipopeptide.
  Journal
ACS Chem Biol 2:187-96 (2007)
DOI:10.1021/cb700012y
  Sequence
[sco:SCO3236]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.39
IUBMB Enzyme Nomenclature: 1.14.11.39
ExPASy - ENZYME nomenclature database: 1.14.11.39
BRENDA, the Enzyme Database: 1.14.11.39

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Chemical substance (6)   
   KEGG COMPOUND (6)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (456)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (360)   
   KEGG MGENES (95)   
Protein sequence (69)   
   UniProt (68)   
   SWISS-PROT (1)   
DNA sequence (50)   
   GenBank (26)   
   EMBL (24)   
Protein domain (3)   
   InterPro (3)   
All databases (586)   

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