KEGG   ENZYME: 1.14.11.39
Entry
EC 1.14.11.39               Enzyme                                 
Name
L-asparagine hydroxylase;
L-asparagine 3-hydroxylase;
AsnO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
L-asparagine,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
L-asparagine + 2-oxoglutarate + O2 = (2S,3S)-3-hydroxyasparagine + succinate + CO2 [RN:R10446]
Reaction(KEGG)
R10446
Substrate
L-asparagine [CPD:C00152];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(2S,3S)-3-hydroxyasparagine [CPD:C20631];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+. The enzyme is only able to hydroxylate free L-asparagine. It is not active toward D-asparagine. The beta-hydroxylated asparagine produced is incorporated at position 9 of the calcium-dependent antibiotic (CDA), an 11-residue non-ribosomally synthesized acidic lipopeptide lactone.
History
EC 1.14.11.39 created 2013
Orthology
K18058  L-asparagine oxygenase
Genes
ENCECL_03968
ECLGEC036_33910
EMORL6Y89_16585
SMACSMDB11_0605 SMDB11_1104
SRRSerAS9_0274
SPLYQ5A_006790(asnO)
SRSSerAS12_0274
SRASerAS13_0273
SFGAV650_20270
SERASer39006_015050
SERQCWC46_15045
SSURATE40_003415
RAQRahaq2_2682
ROXBV494_24950
DSOA4U42_21025
CEDLH89_00095 LH89_22315
DFNCVE23_12695
DAQDAQ1742_04254
DDADd703_1456
ETAETA_15700
PLUplu3528 plu3801
PLUMA4R40_17540 A4R40_18915
PTTVY86_04825
XBVXBW1_mp0174
XNEXNC1_2155 XNC1_2460 XNC1_4045
XNMXNC2_1351 XNC2_2084 XNC2_2366 XNC2_3898
XPOXPG1_2874 XPG1_3240
XHOA9255_19685
HIFHIBPF_07710
HILHICON_17210
HAEGNCTC8502_01634(asnO)
XFMXfasm12_0852
XFLP303_03580 P303_04050
FAUFraau_1426
VTUIX91_10335
VAQFIV01_12890(mppO)
VSRVspart_00116(asnO_1) Vspart_02171(asnO_2)
VSAVSAL_I1305
PMAICF386_12490
PPGPputGB1_5419
PPWPputW619_0540
PPUTL483_02555
PPUNPP4_05200
PSPPSPPH_4996
PSYIMME58_00600
PFEPSF113_0453b
PFBVO64_2148
PSVPVLB_22765
PPVNJ69_17710
PSOSPOS17_3974(PputW619_0540)
PPSLBJP27_20500
PANHHU763_002420
PORYEJA05_00025
PSAPST_3923
PSZPSTAB_3884(mppO)
PSRPSTAA_4073
PSCA458_01685
PSJPSJM300_01810
PSHPsest_0326
PSTUUIB01_01625
PSEDDM292_20880
PCHLLLJ08_01665
PKGLW136_01665
ASHAG8E00_11445
MOIMOVS_02950
MCUNNCTC10297_00742
SHPSput200_2525
PRRAT705_10755 AT705_23320
CEBB0D95_16160
LLOLLO_3359
MPSYCEK71_21840
BTHGMS2017_1703
NCINCTC10296_01180(asnO)
BCNBcen_5513
BCHBcen2424_5877
BCMBcenmc03_6386
BCJBCAS0290
BCEOI35_7306
BCEDDM42_6785
BCEPAPZ15_35315
BPYRABD05_30850 ABD05_32945
BSEMWJ12_20955
BMECWJ16_18780
BSTLBBJ41_33110
BURKDM992_33855
BCAIK788_0005646
HYFDTO96_101676
BBROBAU06_22245
BFZBAU07_22400
BOHAKI39_03465
KIAG8A07_15085
JALBZG29_19805
HHTF506_00300
THITHI_1730
FKUFGKAn22_03770
CFUSCYFUS_003163
RBERBE_0478
RBOA1I_02770
AMIHCO731_01849
BICLMTR13_25260
KVLKVU_PA0021
KVUEIO_3104
QCINCF85_14095
BSIACWD84_18685
BWHA9C19_04265
BACSAUL54_07450
PARHI5S86_03595
PFRIL8956_04705
KEBGXN75_09265
PABSJIR001_15040(asnO_1)
MSAMycsm_07253
CPUCPFRC_03130
CPLCp3995_0633(tauD)
CPGCP316_03250
CPPCpP54B96_0633(tauD)
CPKCP1002_10005
CPQCPC231_03130
CPXCPI19_08390
CPZCpPAT10_0623(tauD)
CORCp267_0650(tauD)
COPCP31_03485
CODCp106_0606(tauD)
COSCp4202_0616(tauD)
COICPCIP5297_03275
COECP258_03270
COUCP162_03155
CPSECPTA_01173
CPSUCPTB_00359
CPSFCPTC_01163
CULCULC22_00672(ogs)
CUCCULC809_00665(ogs)
CUECULC0102_0775(ogs)
CUNCul210932_0700(tauD)
CUSCulFRC11_0669(tauD)
CUQCul210931_0669(tauD)
CUZCul05146_0718(tauD)
CUJCUL131002_0678c(tauD)
CGYCGLY_00545 CGLY_11835
CSILCBE74_03910
NFANFA_48610
NFRERS450000_05049(asnO)
NCYNOCYR_4655
NBRO3I_036890
NNONONO_c66170
NSLBOX37_26935
NSRNS506_01690(asnO)
NTPCRH09_30780 CRH09_34590
NOZDMB37_15820 DMB37_36755
NODFOH10_01930
NAHF5544_17945 F5544_17970 F5544_39350
NADNCTC11293_06239(asnO_2)
NWLNWFMUON74_10480
NIEKV110_36135
ROAPd630_LPD16019
GOMD7316_02856(mppO)
SRTSrot_0774
SCOSCO2693(SCC61A.14) SCO3236(SCE29.05c)
SALBXNR_0986
SGBWQO_12245
SCBSCAB_3341 SCAB_5271
SSXSACTE_6543
SVLStrvi_3124
SHYSHJG_1397
SHOSHJGH_1231
SRCM271_40120
SCTSCAT_2689(asnO) SCAT_p1377
SCYSCATT_26730 SCATT_p03480
SBHSBI_00878 SBI_00941 SBI_09256
SDVBN159_6047
SALSSLNWT_6835
STRPF750_6418
SFISFUL_5831
SCIB446_23870
SALUDC74_5997
SALLSAZ_31060
SLVSLIV_21510(asnO1) SLIV_24200(asnO2)
SGUSGLAU_07120
SVTSVTN_22095
STREGZL_01725
SLDT261_2127 T261_7562
SPRISPRI_3466
SCZABE83_06835 ABE83_06955
SCXAS200_25135
SRWTUE45_06291(mppO_1) TUE45_06409(asnO_5) TUE45_06920(mppO_2) TUE45_pSRc_0447(vioC)
SRNA4G23_02365(asnO_1) A4G23_04203 A4G23_04388(vioC) A4G23_05516
SCLFBB341_15030
STSIA4E84_04350
SKYD0C37_06320 D0C37_30510
SALWCP975_03210 CP975_28170
SLSSLINC_4870
SPLULK06_021015
SNWBBN63_06790 BBN63_30085
SSIAA7J05_00255 A7J05_03135 A7J05_07200 A7J05_36480
SVUB1H20_11480
SGVB1H19_08945 B1H19_09120 B1H19_35575
SLAUSLA_5783
SALJSMD11_0981 SMD11_3232
SLXSLAV_05480(mppO) SLAV_07260(asnO)
SFKKY5_0486c
SGEDWG14_08222(mppO)
SLKSLUN_13775 SLUN_22945
SDXC4B68_01260 C4B68_36880
SGDELQ87_17590
SASTCD934_30580
SNQCP978_01995
STIRDDW44_23825
SKACP970_40345
SGZC0216_32755(vioC)
SVNCP980_28965
SNKCP967_17245
SRKFGW37_28385
SAQUEJC51_46700
SCAVCVT27_26855
SSEOD0Z67_22420
SBYH7H31_00850
SRIMCP984_39215
SPHVF9278_45090
SCINCP977_31230
SCHACP983_18395
SGXH4W23_33125
SBATG4Z16_04870
SNFJYK04_08164(asnO_2)
SPLACP981_21715
SATAC5746_39315
SHUNDWB77_00456(asnO_1)
SLIAHA039_20700
SDWK7C20_27875
SCALI6J39_11685 I6J39_22910
SFBCP974_12965 CP974_22660 CP974_29360
SROIIAG44_03950
SXNIAG42_37060 IAG42_37075
SHKJ2N69_28135
STRYEQG64_28155
SDECL3078_05850
KABB7C62_11150 B7C62_26740
KITCFP65_1227
MSFIT882_06300
RTNA6122_0640
RRYC1O28_01960
RTEGSU10_09185
PNVJMY29_00220
NCANoca_1042
KQIF1D05_30950
SROSros_3755
NOABKM31_45775
FALFRAAL4688
KRAKrad_3958
SENSACE_4278 SACE_5782
AOIAORI_3167
AJAAJAP_23525
AMYCCU254_26135
AMYYYIM_25785(asnO3)
AORISD37_09295 SD37_16155
APRECNX65_18320
SSYIEKG83_30670
KALKALB_1756 KALB_2593 KALB_5896 KALB_6160
LEDBBK82_35715
ACTAC1701_17135 C1701_25725
ALOCRK62029
MAUMicau_1827
MTEMGCE86_18175
VMAVAB18032_03125
MCRAID554_06655 ID554_16940
ASEACPL_2187(cs1)
ACTNL083_4621
ACTSACWT_2065
PLKCIK06_09170(vioC)
PLABC6361_01855 C6361_01950
PLATC6W10_19720 C6W10_19815
PSUUPsuf_065540(asnO)
VERHUT12_06305 HUT12_30735
CAICaci_0231 Caci_6328
RXYRxyl_1155
CYBCYB_1854
LETO77CONTIG1_00553(cs2)
THEUHPC62_14935
GENGM3709_3716
GESVT84_06800(asnO)
CPICpin_2834
CHIHGWR21_13095
NKONiako_2766
FLNFLA_1331
FJOFjoh_3169
CIOCEQ15_06495
CHRYCEY12_03435
CJTEG359_03155
CCAUEG346_19170
CGLENCTC11432_00670(asnO)
 » show all
Reference
1  [PMID:17373765]
  Authors
Strieker M, Kopp F, Mahlert C, Essen LO, Marahiel MA
  Title
Mechanistic and structural basis of stereospecific Cbeta-hydroxylation in calcium-dependent antibiotic, a daptomycin-type lipopeptide.
  Journal
ACS Chem Biol 2:187-96 (2007)
DOI:10.1021/cb700012y
  Sequence
[sco:SCO3236]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.39
IUBMB Enzyme Nomenclature: 1.14.11.39
ExPASy - ENZYME nomenclature database: 1.14.11.39
BRENDA, the Enzyme Database: 1.14.11.39

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