KEGG   ENZYME: 1.14.11.45Help
Entry
EC 1.14.11.45               Enzyme                                 

Name
L-isoleucine 4-hydroxylase;
ido (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
L-isoleucine,2-oxoglutarate:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
L-isoleucine + 2-oxoglutarate + O2 = (4S)-4-hydroxy-L-isoleucine + succinate + CO2 [RN:R10723]
Reaction(KEGG)
Substrate
L-isoleucine [CPD:C00407];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(4S)-4-hydroxy-L-isoleucine [CPD:C20802];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
The enzyme, characterized from the bacterium Bacillus thuringiensis, can also catalyse the hydroxylation of L-leucine, L-norvaline, L-norleucine, and L-allo-isoleucine, as well as the sulfoxidation of L-methionine, L-ethionine, S-methyl-L-cysteine, S-ethyl-L-cysteine, and S-allyl-L-cysteine.
History
EC 1.14.11.45 created 2014
Orthology
K20418  L-isoleucine 4-hydroxylase
Genes
BSL: A7A1_2008
BSO: BSNT_10608
BAE: BATR1942_17630
BATR: TD68_16535
BCE: BC1061
BCB: BCB4264_A1096
BCG: BCG9842_B4209
BTT: HD73_1215
BTHR: YBT1520_06070
BTHI: BTK_06120
BTI: BTG_15640
BJS: MY9_4045
 » show all
Taxonomy
Reference
1  [PMID:19850012]
  Authors
Kodera T, Smirnov SV, Samsonova NN, Kozlov YI, Koyama R, Hibi M, Ogawa J, Yokozeki K, Shimizu S
  Title
A novel l-isoleucine hydroxylating enzyme, l-isoleucine dioxygenase from Bacillus thuringiensis, produces (2S,3R,4S)-4-hydroxyisoleucine.
  Journal
Biochem Biophys Res Commun 390:506-10 (2009)
DOI:10.1016/j.bbrc.2009.09.126
  Sequence
Reference
2  [PMID:21821743]
  Authors
Hibi M, Kawashima T, Kodera T, Smirnov SV, Sokolov PM, Sugiyama M, Shimizu S, Yokozeki K, Ogawa J
  Title
Characterization of Bacillus thuringiensis L-isoleucine dioxygenase for production of useful amino acids.
  Journal
Appl Environ Microbiol 77:6926-30 (2011)
DOI:10.1128/AEM.05035-11
  Sequence
Reference
3
  Authors
Hibi, M., Kawashima, T., Yajima, H., Smirnov, S.V., Kodera, T., Sugiyama, M., Shimizu, S., Yokozeki, K., and Ogawa, J.
  Title
Enzymatic synthesis of chiral amino acid sulfoxides by Fe(II)/alpha ketoglutarate-dependent dioxygenase.
  Journal
Tetrahedron Asym 24:990-994 (2013)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.45
IUBMB Enzyme Nomenclature: 1.14.11.45
ExPASy - ENZYME nomenclature database: 1.14.11.45
BRENDA, the Enzyme Database: 1.14.11.45

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