KEGG   ENZYME: 1.14.11.48Help
Entry
EC 1.14.11.48               Enzyme                                 

Name
xanthine dioxygenase;
XanA;
alpha-ketoglutarate-dependent xanthine hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
xanthine,2-oxoglutarate:oxygen oxidoreductase
Reaction(IUBMB)
xanthine + 2-oxoglutarate + O2 = urate + succinate + CO2 [RN:R10920]
Reaction(KEGG)
Substrate
xanthine [CPD:C00385];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
urate [CPD:C00366];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and L-ascorbate. The enzyme, which was characterized from fungi, is specific for xanthine.
History
EC 1.14.11.48 created 2015
Reference
1  [PMID:15948966]
  Authors
Cultrone A, Scazzocchio C, Rochet M, Montero-Moran G, Drevet C, Fernandez-Martin R
  Title
Convergent evolution of hydroxylation mechanisms in the fungal kingdom: molybdenum cofactor-independent hydroxylation of xanthine via alpha-ketoglutarate-dependent dioxygenases.
  Journal
Mol Microbiol 57:276-90 (2005)
DOI:10.1111/j.1365-2958.2005.04686.x
Reference
2  [PMID:17429948]
  Authors
Montero-Moran GM, Li M, Rendon-Huerta E, Jourdan F, Lowe DJ, Stumpff-Kane AW, Feig M, Scazzocchio C, Hausinger RP
  Title
Purification and characterization of the FeII- and alpha-ketoglutarate-dependent  xanthine hydroxylase from Aspergillus nidulans.
  Journal
Biochemistry 46:5293-304 (2007)
DOI:10.1021/bi700065h
Reference
3  [PMID:18036331]
  Authors
Li M, Muller TA, Fraser BA, Hausinger RP
  Title
Characterization of active site variants of xanthine hydroxylase from Aspergillus nidulans.
  Journal
Arch Biochem Biophys 470:44-53 (2008)
DOI:10.1016/j.abb.2007.11.002
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.48
IUBMB Enzyme Nomenclature: 1.14.11.48
ExPASy - ENZYME nomenclature database: 1.14.11.48
BRENDA, the Enzyme Database: 1.14.11.48

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