KEGG   ENZYME: 1.14.11.52Help
Entry
EC 1.14.11.52               Enzyme                                 

Name
validamycin A dioxygenase;
vldW (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
validamycin-A,2-oxoglutarate:oxygen oxidoreductase (6'-hydroxylating)
Reaction(IUBMB)
validamycin A + 2-oxoglutarate + O2 = validamycin B + succinate + CO2 [RN:R11340]
Reaction(KEGG)
Substrate
validamycin A [CPD:C12112];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
validamycin B [CPD:C21293];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
The enzyme was characterized from the bacterium Streptomyces hygroscopicus subsp. limoneus. Requires Fe2+.
History
EC 1.14.11.52 created 2016
Pathway
ec00525  Acarbose and validamycin biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K21036  validamycin A dioxygenase
Genes
SHY: SHJG_0280 SHJG_0285
SHO: SHJGH_0114 SHJGH_0119
Taxonomy
Reference
1  [PMID:22961651]
  Authors
Almabruk KH, Asamizu S, Chang A, Varghese SG, Mahmud T
  Title
The alpha-ketoglutarate/Fe(II)-dependent dioxygenase VldW is responsible for the  formation of validamycin B.
  Journal
Chembiochem 13:2209-11 (2012)
DOI:10.1002/cbic.201200464
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.52
IUBMB Enzyme Nomenclature: 1.14.11.52
ExPASy - ENZYME nomenclature database: 1.14.11.52
BRENDA, the Enzyme Database: 1.14.11.52

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