KEGG   ENZYME: 1.14.11.56Help
Entry
EC 1.14.11.56               Enzyme                                 

Name
L-proline cis-4-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
L-proline,2-oxoglutarate:oxygen oxidoreductase (cis-4-hydroxylating)
Reaction(IUBMB)
L-proline + 2-oxoglutarate + O2 = cis-4-hydroxy-L-proline + succinate + CO2 [RN:R11594]
Reaction(KEGG)
Substrate
L-proline [CPD:C00148];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
cis-4-hydroxy-L-proline [CPD:C01015];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. The enzyme, isolated from Rhizobium species, only produces cis-4-hydroxy-L-proline (cf. EC 1.14.11.57, L-proline trans-4-hydroxylase).
History
EC 1.14.11.56 created 2017
Orthology
K21614  L-proline cis-4-hydroxylase
Genes
MLO: mlr6283
SME: SMc03253
SMEL: SM2011_c03253
Taxonomy
Reference
1  [PMID:19133227]
  Authors
Hara R, Kino K
  Title
Characterization of novel 2-oxoglutarate dependent dioxygenases converting L-proline to cis-4-hydroxy-l-proline.
  Journal
Biochem Biophys Res Commun 379:882-6 (2009)
DOI:10.1016/j.bbrc.2008.12.158
  Sequence
[sme:SMc03253] [mlo:mlr6283]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.56
IUBMB Enzyme Nomenclature: 1.14.11.56
ExPASy - ENZYME nomenclature database: 1.14.11.56
BRENDA, the Enzyme Database: 1.14.11.56

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