Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

thymine,2-oxoglutarate:oxygen oxidoreductase (7-hydroxylating)

thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2 [RN:R01412]

R01412;
(other) R08610 R08611

thymine [CPD:C00178];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]

Requires Fe2+ and ascorbate. Also acts on 5-hydroxymethyluracil to oxidize its -CH2OH group first to -CHO and then to -COOH.

EC 1.14.11.6 created 1972, modified 1976 (EC 1.14.11.5 created 1972, incorporated 1976)

1  [PMID:11946494]

Bankel L, Holme E, Lindstedt G, Lindstedt S.

Oxygenases involved in thymine and thymidine metabolism in Neurospora crassa.

FEBS Lett 21:135-138 (1972)
DOI:10.1016/0014-5793(72)80121-2

2  [PMID:4274083]

Liu CK, Hsu CA, Abbott MT.

Catalysis of three sequential dioxygenase reactions by thymine 7-hydroxylase.

Arch Biochem Biophys 159:180-7 (1973)
DOI:10.1016/0003-9861(73)90443-8

3  [PMID:6684117]

Warn-Cramer BJ, Macrander LA, Abbott MT.

Markedly different ascorbate dependencies of the sequential alpha-ketoglutarate dioxygenase reactions catalyzed by an essentially homogeneous thymine 7-hydroxylase from Rhodotorula glutinis.

J Biol Chem 258:10551-7 (1983)