KEGG   ENZYME: 1.14.11.60
Entry
EC 1.14.11.60               Enzyme                                 
Name
scopoletin 8-hydroxylase;
S8H (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
scopoletin,2-oxoglutarate:oxygen oxidoreductase (8-hydroxylating)
Reaction(IUBMB)
scopoletin + 2-oxoglutarate + O2 = fraxetin + succinate + CO2 [RN:R12222]
Reaction(KEGG)
R12222
Substrate
scopoletin [CPD:C01752];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
fraxetin [CPD:C09265];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires iron(II) and ascorbate. A protein involved in biosynthesis of iron(III)-chelating coumarins in higher plants.
History
EC 1.14.11.60 created 2018
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01110  Biosynthesis of secondary metabolites
Orthology
K23145  scopoletin 8-hydroxylase
Genes
ATHAT3G12900
ALY9318860
CRB17892971
CSAT104745510 104748679 104765057 104777120
EUSEUTSA_v10020841mg
BRP103849720 103859475 103870212
BNA106345421 106375087 106375542 106382146 106386876 106400351
BOE106293989 106301500 106333669
RSZ108810911 108835958 108861046
THJ104810262
CPAP110809857
CIT102607640
CICCICLE_v10006882mg
PVY116119197 116133563
MINC123206691 123226450
TCC18594550 18594551 18594553 18594556
GRA105761379 105775787 105775819 105784310 105784311 105787151 105787154
GHI107886197 107911338 107912932 107913465 107919665 107932640 107932661 107932662 107936166 107936167 107936168 107936170 121223450
GAB108452509 108461393 108461394 108480494 108480495 108480496 108480497 108482454 108482635
DZI111276015 111276016 111276018 111276020 111276597 111276599 111276600 111287890 111318382 111318388 111318392
GMX100803722 102659647
GSJ114423069 114423906
PVUPHAVU_005G059100g
VRA106761811
VAR108333781
VUN114181474
CCAJ109790572 109790573 109790576 109818815
MTRMTR_2g068800 MTR_2g068840 MTR_2g068850
CAM101492926
LJALj3g3v0965390.1(Lj3g3v0965390.1) Lj3g3v0965390.2(Lj3g3v0965390.2) Lj3g3v0965390.3(Lj3g3v0965390.3)
ADU107466129 107471942 107480135
AIP107619811 107630421
AHF112708410 112711180 112738254 112779478 112791023
LANG109333837 109355985
FVE105350415
RCN112203388
PPER18778908
PMUM103325695
PAVI110768994
PDUL117626205
MDM103435419 114823354
PXB103952012
MNT21399318
RCU107260838 8272615
JCU105646195 105646196
HBR110638461 110662018 110662019 110662020
MESC110617017 110630845 122721679 122723798
POP7480442
PEU105123997
PALZ118054079
JRE109007025
QSU111986809 112009979
QLO115984049 115984050
TWL120009221
VVI100259503 100854658 104877301
VRI117908689 117908690 117910864
SLY101262174
SPEN107004094
SOT102584589
NTA107766774 107821681
NSY104226661
NTO104118671
NAU109238378
INI109160293
ITR116005047
OEU111396786
HAN110878125 110878126 110878127 110937900 110939629 110939645 110939654 110939655
ECAD122594033
LSV111890983 111890990 111891065 111902368
CCAV112522925
DCR108216893 108218127
CSIN114267034
NNU104603880 104609462
MING122059183 122063766 122063776 122067316 122092711
TSS122640930 122649187
PSOM113289572 113298261 113298269 113326671 113332375
ATR18424098 18424099 18424100 18424103
 » show all
Reference
1  [PMID:29361149]
  Authors
Siwinska J, Siatkowska K, Olry A, Grosjean J, Hehn A, Bourgaud F, Meharg AA, Carey M, Lojkowska E, Ihnatowicz A
  Title
Scopoletin 8-hydroxylase: a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis.
  Journal
J Exp Bot 69:1735-1748 (2018)
DOI:10.1093/jxb/ery005
  Sequence
[ath:AT3G12900]
Reference
2  [PMID:29581584]
  Authors
Rajniak J, Giehl RFH, Chang E, Murgia I, von Wiren N, Sattely ES
  Title
Biosynthesis of redox-active metabolites in response to iron deficiency in plants.
  Journal
Nat Chem Biol 14:442-450 (2018)
DOI:10.1038/s41589-018-0019-2
  Sequence
[ath:AT3G12900]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.60
IUBMB Enzyme Nomenclature: 1.14.11.60
ExPASy - ENZYME nomenclature database: 1.14.11.60
BRENDA, the Enzyme Database: 1.14.11.60

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