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Entry
EC 1.14.11.60               Enzyme                                 

Name
scopoletin 8-hydroxylase;
S8H (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
scopoletin,2-oxoglutarate:oxygen oxidoreductase (8-hydroxylating)
Reaction(IUBMB)
scopoletin + 2-oxoglutarate + O2 = fraxetin + succinate + CO2 [RN:R12222]
Reaction(KEGG)
Substrate
scopoletin [CPD:C01752];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
fraxetin [CPD:C09265];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires iron(II) and ascorbate. A protein involved in biosynthesis of iron(III)-chelating coumarins in higher plants.
History
EC 1.14.11.60 created 2018
Orthology
K23145  scopoletin 8-hydroxylase
Genes
ATH: AT3G12900
ALY: ARALYDRAFT_341402
CRB: 17892971
CSAT: 104745510 104748162 104748679 104765057 104777120 109129070
EUS: EUTSA_v10020841mg
BRP: 103859475 103870212
BNA: 106345421 106375087 106375542 106382146 106386876 106400351
BOE: 106293989 106301500 106333669
RSZ: 108810911 108835958 108861046
THJ: 104810262
CPAP: 110809857
CIT: 102607640
GMX: 100803722
VRA: 106761811
VAR: 108333781
VUN: 114181474
CAM: 101492926
LJA: Lj3g3v0965390.1(Lj3g3v0965390.1) Lj3g3v0965390.2(Lj3g3v0965390.2) Lj3g3v0965390.3(Lj3g3v0965390.3)
FVE: 105350415
RCN: 112203388
PPER: 18778908
PMUM: 103325695
PAVI: 110768994
PXB: 103952012
POP: 7480442
JRE: 109007025
SLY: 101262174
SPEN: 107004094
SOT: 102584589
NSY: 104226661
NTO: 104118671
NAU: 109238378
INI: 109160293
OEU: 111396786
CCAV: 112522925
 » show all
Taxonomy
Reference
1  [PMID:29361149]
  Authors
Siwinska J, Siatkowska K, Olry A, Grosjean J, Hehn A, Bourgaud F, Meharg AA, Carey M, Lojkowska E, Ihnatowicz A
  Title
Scopoletin 8-hydroxylase: a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis.
  Journal
J Exp Bot 69:1735-1748 (2018)
DOI:10.1093/jxb/ery005
  Sequence
[ath:AT3G12900]
Reference
2  [PMID:29581584]
  Authors
Rajniak J, Giehl RFH, Chang E, Murgia I, von Wiren N, Sattely ES
  Title
Biosynthesis of redox-active metabolites in response to iron deficiency in plants.
  Journal
Nat Chem Biol 14:442-450 (2018)
DOI:10.1038/s41589-018-0019-2
  Sequence
[ath:AT3G12900]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.60
IUBMB Enzyme Nomenclature: 1.14.11.60
ExPASy - ENZYME nomenclature database: 1.14.11.60
BRENDA, the Enzyme Database: 1.14.11.60

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