EC                Enzyme                                 

procollagen-proline 3-dioxygenase;
proline,2-oxoglutarate 3-dioxygenase;
prolyl 3-hydroxylase;
protocollagen proline 3-hydroxylase;
prolyl-4-hydroxyprolyl-glycyl-peptide,2-oxoglutarate:oxygen oxidoreductase, 3-hydroxylating
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
[procollagen]-L-proline,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
[procollagen]-L-proline + 2-oxoglutarate + O2 = [procollagen]-trans-3-hydroxy-L-proline + succinate + CO2 [RN:R03218]
[procollagen]-L-proline [CPD:C01078];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
[procollagen]-trans-3-hydroxy-L-proline [CPD:C04397];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Requires Fe2+ and ascorbate. The enzyme forms a complex with protein disulfide isomerase, and is located in the endoplasmic reticulum. It modifies proline residues within the procollagen peptide of certain collagen types. The modification is essential for proper collagen triple helix formation.
EC created 1981, modified 1983, modified 2017
K08134  procollagen-proline 3-dioxygenase 1
K22459  procollagen-proline 3-dioxygenase 2
K22460  procollagen-proline 3-dioxygenase 3
HSA: 10536(P3H3) 55214(P3H2) 64175(P3H1)
PTR: 100612169(P3H3) 456811(P3H1) 740124(P3H2)
PPS: 100967913(P3H3) 100988847(P3H2) 100995838(P3H1)
GGO: 101124810(P3H2) 101134181(P3H3) 101141437(P3H1)
PON: 100445803(P3H2) 100452759(P3H3) 100454619(P3H1)
NLE: 100579995(P3H2) 100580431(P3H1) 100592815(P3H3)
MCC: 697917(P3H1) 704118(P3H2) 713865(P3H3)
MCF: 102115049(P3H1) 102125709(P3H3) 102137302(P3H2)
CSAB: 103218477(P3H3) 103224997(P3H1) 103241897(P3H2)
RRO: 104669007(P3H1) 104680052(P3H2) 104680134(P3H3)
RBB: 108530367(P3H1) 108530476(P3H2) 108542032(P3H3)
CJC: 100385724(P3H3) 100388266(P3H2) 100402370(P3H1)
SBQ: 101029883 101038436(LEPREL1) 101052278(LEPRE1)
MMU: 14789(P3h3) 210530(P3h2) 56401(P3h1)
RNO: 114200(P3h1) 288016(P3h2) 297595(P3h3)
CGE: 100752370(P3h2) 100767056(P3h3) 100769529(P3h1)
NGI: 103727504(P3h3) 103739336(P3h2) 103741906(P3h1)
HGL: 101709763(P3h2) 101719240(P3h3) 101725544(P3h1)
CCAN: 109678351(P3h2) 109682771(P3h3) 109691192(P3h1)
OCU: 100344567(P3H3) 100356128(P3H2) 100357143(P3H1)
TUP: 102468745(P3H1) 102489939(P3H3) 102490664(P3H2)
CFA: 100686626(P3H3) 478674(P3H2) 606980(P3H1)
AML: 100477435(P3H1) 100482440(P3H3) 100484169(P3H2)
UMR: 103656188(P3H3) 103670920(P3H1) 103676024(P3H2)
ORO: 101366397(P3H1) 101370866(P3H3) 101373687(P3H2)
FCA: 101093365(P3H3) 101094278(P3H1) 101094873(P3H2)
PTG: 102950281(P3H2) 102955345(P3H3) 102958232(P3H1)
AJU: 106965441(P3H3) 106968016(P3H2) 106970202(P3H1)
BTA: 511799(P3H2) 539976(P3H1)
BOM: 102280597(P3H1) 102284601(P3H2) 102287577(P3H3)
BIU: 109556425(P3H1) 109556764(P3H2) 109558918(P3H3)
PHD: 102332498(P3H1) 102333393(P3H3) 102343352(P3H2)
CHX: 102176848(P3H1) 102188119(P3H3) 102189992(P3H2)
OAS: 101105862(P3H1) 101108618(P3H2) 101108826(P3H3)
SSC: 100156539(P3H3) 100520219(P3H2) 100620503(P3H1)
CFR: 102510969(P3H3) 102516638(P3H2) 102516978(P3H1)
CDK: 105097544(P3H2) 105098205(P3H3) 105103354(P3H1)
BACU: 103005242(P3H2) 103015069(P3H3) 103015131(P3H1)
LVE: 103076723(P3H2) 103081402(P3H1) 103090013(P3H3)
OOR: 101273511(P3H1) 101285801(P3H3) 101289013(P3H2)
ECB: 100059777(P3H2) 100060032(P3H3) 100067315(P3H1)
EPZ: 103547016(P3H3) 103557310(P3H1) 103564888(P3H2)
EAI: 106832556(P3H2) 106847326(P3H3) 106847794(P3H1)
MYB: 102239815(P3H1) 102245885(P3H2) 102248956(P3H3)
MYD: 102755248(P3H3) 102759591(P3H2) 102764443(P3H1)
HAI: 109371222(P3H2) 109373668(P3H3) 109383352(P3H1)
RSS: 109453445(P3H2) 109457864(P3H1) 109459705(P3H3)
PALE: 102884197(P3H1) 102890385(P3H2) 102893257(P3H3)
LAV: 100658405(P3H2) 100665625(P3H1) 100675290(P3H3)
MDO: 100016121(P3H3) 100032694(P3H1) 100618320(P3H2)
SHR: 100914814(P3H1) 100923448(P3H3) 100933913(P3H2)
OAA: 100081783(P3H2) 100085351(P3H1)
GGA: 414142(P3H1) 414143(P3H2) 418289(P3H3)
MGP: 100546471(P3H1) 100548323(P3H3) 100549834(P3H2)
CJO: 107318275(P3H2) 107318675(P3H3) 107323389(P3H1)
APLA: 101793572(P3H1) 101794735(P3H3) 101795167(P3H2)
ACYG: 106044830(P3H2) 106047855(P3H3) 106049155(P3H1)
TGU: 100221140(P3H3) 100221254(P3H1) 100224069(P3H2)
SCAN: 103812452(P3H3) 103815383(P3H2) 103824173(P3H1)
GFR: 102036321(P3H1) 102040203(P3H3) 102040654(P3H2)
FAB: 101810645(P3H3) 101821011(P3H2) 101821087(P3H1)
PHI: 102099220(P3H2) 102106882(P3H1) 102112679(P3H3)
PMAJ: 107208918(P3H2) 107213395(P3H3) 107213791(P3H1)
CCAE: 111933453(P3H2) 111938269(P3H3) 111938574(P3H1)
CCW: 104687224(P3H3) 104687928(P3H2) 104697539(P3H1)
FPG: 101917584(P3H2) 101919025(P3H3) 101923126(P3H1)
FCH: 102048595(P3H1) 102050123(P3H2) 102055974(P3H3)
CLV: 102084407(P3H3) 102094017(P3H1) 102097675(P3H2)
EGZ: 104123376(LEPREL1) 104126599(LEPREL2) 104131732(LEPRE1)
NNI: 104011258(LEPRE1) 104015666(LEPREL1) 104022495(LEPREL2)
ACUN: 113476602(P3H3) 113483556(P3H2) 113487825(P3H1)
AAM: 106482208 106497271(P3H3) 106498214(P3H2) 106498600(P3H1)
AMJ: 102562134(P3H3) 102572352(P3H2) 106737225(P3H1)
PSS: 102444832(P3H2) 102450598(P3H1) 102461480(P3H3)
CMY: 102929854(P3H3) 102932162(P3H1) 102939997(P3H2)
CPIC: 101931004(LEPREL2) 101931818(P3H2) 101935857(P3H1)
ACS: 100551581 100562772(p3h2) 100567613(p3h3)
PVT: 110084656(P3H3) 110085947(P3H2) 110086810(P3H1)
PBI: 103048096(P3H2) 103049535(P3H3) 103066967(P3H1)
PMUR: 107285772(P3H3) 107290023(P3H2) 107299512
GJA: 107116689(P3H1) 107116803(P3H3) 107123825(P3H2)
XLA: 108697338(p3h3.S) 108716969 108718050 414447(p3h1.L)
NPR: 108783932(P3H1) 108795998(P3H3) 108804588(P3H2)
DRE: 553311(p3h3) 553321(p3h1) 566697(p3h2)
IPU: 108270766(p3h2) 108274273(p3h3) 108281059(p3h1)
PHYP: 113537803(p3h1) 113542320(p3h3) 113547067(p3h2)
AMEX: 103024133(p3h1) 103026715(p3h3) 103045377(p3h2)
EEE: 113570617(p3h3) 113582372(p3h2) 113586117(p3h1)
TRU: 101075372(p3h3) 101075489(p3h1) 101076075(p3h2)
LCO: 104920250(p3h2) 104922230(p3h3) 104939521(p3h1)
MZE: 101465294(p3h1) 101479402(p3h2) 101486114(p3h3)
OLA: 101170663(p3h2) 101171860(p3h3) 101172073(p3h1)
XMA: 102223713(p3h2) 102234680(p3h3) 102238287(p3h1)
PRET: 103464183(p3h2) 103465445(p3h1) 103477971(p3h3)
NFU: 107373178 107379477(p3h3) 107392670(p3h2) 107396976(p3h1)
KMR: 108230631 108235396(p3h3) 108250443(p3h1)
CSEM: 103386606(p3h1) 103387968(p3h3) 103392998(p3h2)
LCF: 108877527(p3h1) 108880725(p3h3) 108881307(p3h2)
SDU: 111217020(p3h2) 111221750(p3h1) 111230047(p3h3)
HCQ: 109515135(p3h1) 109515281(p3h3) 109522012(p3h2)
BPEC: 110160074(p3h2) 110163193(p3h3) 110171175(p3h1)
ELS: 105007738(p3h2) 105028240(p3h3) 105030006(p3h1)
SFM: 108922091(p3h3) 108937368(p3h2) 108938176(p3h1)
LCM: 102348936(P3H3) 102353193(P3H2) 102356877(P3H1) 102366409
CMK: 103181352(p3h1) 103191590(p3h2)
APLC: 110983136
SKO: 100313646
AME: 725819
BIM: 100740426
BTER: 100650141
SOC: 105199999
MPHA: 105840059
AEC: 105148061
ACEP: 105624115
PBAR: 105427498
HST: 105190225
DQU: 106747649
CFO: 105259052
LHU: 105679345
PGC: 109859021
OBO: 105282343
PCF: 106788117
NVI: 100123941
MDL: 103575387
TCA: 657387
NVL: 108566762
ZNE: 110827209
LAK: 106160773
EPA: 110243698
ADF: 107344196
PDAM: 113667901
HMG: 100203735
AQU: 100638799
GHI: 107898752
EGR: 104433013
GMX: 100788718
VRA: 106768895
VAR: 108347895
LANG: 109359831
PPER: 18791527
ZJU: 107405477
CSV: 101218213
CMO: 103496668
CMAX: 111487019
RCU: 8272312
LSV: 111917745
PPP: 112278355
 » show all
1  [PMID:191255]
Risteli J, Tryggvason K, Kivirikko KI.
Prolyl 3-hydroxylase: partial characterization of the enzyme from rat kidney cortex.
Eur J Biochem 73:485-92 (1977)
2  [PMID:204218]
Risteli J, Tryggvason K, Kivirikko KI.
A rapid assay for prolyl 3-hydroxylase activity.
Anal Biochem 84:423-31 (1978)
3  [PMID:15044469]
Vranka JA, Sakai LY, Bachinger HP
Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes.
J Biol Chem 279:23615-21 (2004)
4  [PMID:18487197]
Tiainen P, Pasanen A, Sormunen R, Myllyharju J
Characterization of recombinant human prolyl 3-hydroxylase isoenzyme 2, an enzyme modifying the basement membrane collagen IV.
J Biol Chem 283:19432-9 (2008)
Other DBs
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IUBMB Enzyme Nomenclature:
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CAS: 63551-75-7

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