KEGG   ENZYME: 1.14.11.70
Entry
EC 1.14.11.70               Enzyme                                 

Name
7-deoxycylindrospermopsin hydroxylase;
cyrI (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
7-deoxycylindrospermopsin,2-oxoglutarate:oxygen oxidoreductase (7-hydroxylating)
Reaction(IUBMB)
(1) 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 = cylindrospermopsin + succinate + CO2 [RN:R12509];
(2) 7-deoxycylindrospermopsin + 2-oxoglutarate + O2 = 7-epi-cylindrospermopsin + succinate + CO2 [RN:R12510]
Reaction(KEGG)
R12509 R12510
Substrate
7-deoxycylindrospermopsin [CPD:C22221];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
cylindrospermopsin [CPD:C19999];
succinate [CPD:C00042];
CO2 [CPD:C00011];
7-epi-cylindrospermopsin [CPD:C22222]
Comment
Requires Fe(II). The enzyme, found in some cyanobacterial species, catalyses the last step in the biosynthesis of the toxins cylindrospermopsin and 7-epi-cylindrospermopsin. The ratio of the two products differs among different strains.
History
EC 1.14.11.70 created 2019
Orthology
K24001  7-deoxycylindrospermopsin hydroxylase
Reference
1  [PMID:21404419]
  Authors
Mazmouz R, Chapuis-Hugon F, Pichon V, Mejean A, Ploux O
  Title
The last step of the biosynthesis of the cyanotoxins cylindrospermopsin and 7-epi-cylindrospermopsin is catalysed by CyrI, a 2-Oxoglutarate-dependent iron oxygenase.
  Journal
Chembiochem 12:858-62 (2011)
DOI:10.1002/cbic.201000726
Reference
2  [PMID:29653078]
  Authors
Mazmouz R, Essadik I, Hamdane D, Mejean A, Ploux O
  Title
Characterization of CyrI, the hydroxylase involved in the last step of cylindrospermopsin biosynthesis: Binding studies, site-directed mutagenesis and stereoselectivity.
  Journal
Arch Biochem Biophys 647:1-9 (2018)
DOI:10.1016/j.abb.2018.04.007
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.70
IUBMB Enzyme Nomenclature: 1.14.11.70
ExPASy - ENZYME nomenclature database: 1.14.11.70
BRENDA, the Enzyme Database: 1.14.11.70

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