KEGG   ENZYME: 1.14.11.74
Entry
EC 1.14.11.74               Enzyme                                 
Name
L-isoleucine 31-dioxygenase;
hilA (gene name);
L-isoleucine 4'-dioxygenase (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
L-isoleucine,2-oxoglutarate:oxygen oxidoreductase (31-hydroxylating)
Reaction(IUBMB)
L-isoleucine + 2-oxoglutarate + O2 = 31-hydroxy-L-isoleucine + succinate + CO2 [RN:R12650]
Reaction(KEGG)
R12650
Substrate
L-isoleucine [CPD:C00407];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
31-hydroxy-L-isoleucine;
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. The enzyme has been characterized from the bacterium Pantoea ananatis.
History
EC 1.14.11.74 created 2020
Orthology
K24448  L-isoleucine 31-dioxygenase
Genes
RAQRahaq2_0877
RVCJ9880_11720
RBONQNM34_04185(hilA)
PPUJE2566_20105
PAMPANA_3818
PLFPANA5342_0220
PAJPAJ_3036
PAQPAGR_g0215
XNEXNC1_0854
XNMXNC2_0839
PSPPSPPH_3987
 » show all
Reference
1  [PMID:23554367]
  Authors
Smirnov SV, Sokolov PM, Kotlyarova VA, Samsonova NN, Kodera T, Sugiyama M, Torii T, Hibi M, Shimizu S, Yokozeki K, Ogawa J
  Title
A novel l-isoleucine-4'-dioxygenase and l-isoleucine dihydroxylation cascade in Pantoea ananatis.
  Journal
Microbiologyopen 2:471-81 (2013)
DOI:10.1002/mbo3.87
  Sequence
[paj:PAJ_3036]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.74
IUBMB Enzyme Nomenclature: 1.14.11.74
ExPASy - ENZYME nomenclature database: 1.14.11.74
BRENDA, the Enzyme Database: 1.14.11.74

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