KEGG   ENZYME: 1.14.11.75
Entry
EC 1.14.11.75               Enzyme                                 

Name
31-hydroxy-L-isoleucine 4-dioxygenase;
hilB (gene name);
4'-hydroxy-L-isoleucine 4-dioxygenase (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
Sysname
31-hydroxy-L-isoleucine,2-oxoglutarate:oxygen oxidoreductase (4S-hydroxylating)
Reaction(IUBMB)
31-hydroxy-L-isoleucine + 2-oxoglutarate + O2 = (4S)-31,4-dihydroxy-L-isoleucine + succinate + CO2 [RN:R12651]
Reaction(KEGG)
R12651
Substrate
31-hydroxy-L-isoleucine [CPD:C22300];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(4S)-31,4-dihydroxy-L-isoleucine [CPD:C22301];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. The enzyme has been characterized from the bacterium Pantoea ananatis.
History
EC 1.14.11.75 created 2020
Orthology
K24449  31-hydroxy-L-isoleucine 4-dioxygenase
Genes
RAQ: Rahaq2_0878
PPUJ: E2566_20110
PAM: PANA_3819
PLF: PANA5342_0219
PAJ: PAJ_3037
PAQ: PAGR_g0214
XNE: XNC1_0853
XNM: XNC2_0838
PSP: PSPPH_3986
Reference
1  [PMID:23554367]
  Authors
Smirnov SV, Sokolov PM, Kotlyarova VA, Samsonova NN, Kodera T, Sugiyama M, Torii T, Hibi M, Shimizu S, Yokozeki K, Ogawa J
  Title
A novel l-isoleucine-4'-dioxygenase and l-isoleucine dihydroxylation cascade in Pantoea ananatis.
  Journal
Microbiologyopen 2:471-81 (2013)
DOI:10.1002/mbo3.87
  Sequence
[paj:PAJ_3037]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.75
IUBMB Enzyme Nomenclature: 1.14.11.75
ExPASy - ENZYME nomenclature database: 1.14.11.75
BRENDA, the Enzyme Database: 1.14.11.75

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