KEGG   ENZYME: 1.14.12.10
Entry
EC 1.14.12.10               Enzyme                                 

Name
benzoate 1,2-dioxygenase;
benzoate hydroxylase;
benzoate hydroxylase;
benzoic hydroxylase;
benzoate dioxygenase;
benzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating, decarboxylating) [incorrect]
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
Sysname
benzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Reaction(IUBMB)
benzoate + NADH + H+ + O2 = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ [RN:R07188]
Reaction(KEGG)
Substrate
benzoate [CPD:C00180];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate [CPD:C06321];
NAD+ [CPD:C00003]
Comment
A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), and an iron-sulfur oxygenase. Requires Fe2+.
History
EC 1.14.12.10 created 1972 as EC 1.13.99.2, transferred 1992 to EC 1.14.12.10
Pathway
ec00362  Benzoate degradation
ec00364  Fluorobenzoate degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K05549  benzoate/toluate 1,2-dioxygenase subunit alpha
K05550  benzoate/toluate 1,2-dioxygenase subunit beta
Genes
EFE: EFER_1482(benA) EFER_1483(benB)
KPN: KPN_01871 KPN_01872
KPU: KP1_2936(hcaF) KP1_2937(hcaE)
KPM: KPHS_28410 KPHS_28420
KPP: A79E_2373 A79E_2374
KPH: KPNIH24_14195 KPNIH24_14200
KPZ: KPNIH27_13580 KPNIH27_13585
KPV: KPNIH29_14250 KPNIH29_14255
KPW: KPNIH30_14515 KPNIH30_14520
KPY: KPNIH31_13530 KPNIH31_13535
KPT: VK055_0611(cbdA) VK055_0612(benB)
KPR: KPR_2935(benB) KPR_2937
KPX: PMK1_04212(cbdB) PMK1_04213(cbdA_2)
KPNK: BN49_2971(benB) BN49_2972(benA)
KPE: KPK_2484(cbeA) KPK_2485(cbeB)
PAE: PA2083 PA2517(xylY) PA2518(xylX)
PAEV: N297_2588(benB) N297_2589(benA)
PAEI: N296_2588(benB) N296_2589(benA)
PAU: PA14_32080(xylX) PA14_32100(xylY) PA14_37570
PAP: PSPA7_2719(benA) PSPA7_2720(benB)
PAG: PLES_27771(xylX) PLES_27781(xylY) PLES_32381
PAF: PAM18_2521(xylX) PAM18_2522(xylY)
PNC: NCGM2_3522(xylY) NCGM2_3523(xylX)
PAEB: NCGM1900_4009(xylX) NCGM1900_4010(xylY)
PAEU: BN889_02741(xylY) BN889_02742(xylX)
PAEC: M802_2585(benB) M802_2586(benA)
PAEO: M801_2453(benB) M801_2454(benA)
PRE: PCA10_30930(benB) PCA10_30940(benA)
PCQ: PcP3B5_23330(cbdA) PcP3B5_23340(cbdB)
PPU: PP_3161(benA) PP_3162(benB)
PPX: T1E_1062(benB) T1E_1063(benA)
PPUN: PP4_25370(benA) PP4_25380(benB) PP4_48200(benB) PP4_48210(benA)
PFL: PFL_3857(xylY) PFL_3858(xylX)
PPRC: PFLCHA0_c39150(xylY2) PFLCHA0_c39160(xylX2)
PPRO: PPC_3953(xylYI) PPC_3954
PFE: PSF113_2896(benB) PSF113_2897(benA)
PEN: PSEEN3142(benB) PSEEN3143(benA)
PSA: PST_1667(benA) PST_1668(benB)
PSZ: PSTAB_1578(benA) PSTAB_1579(benB)
PSR: PSTAA_1695(benA) PSTAA_1696(benB)
PPUU: PputUW4_03256(benB) PputUW4_03257(benA)
PSV: PVLB_12215(benA) PVLB_12220(benB)
PKC: PKB_2100(xylX) PKB_2101(xylY)
PSES: PSCI_4808(xylYI) PSCI_4809
PSOS: POS17_3978(xylYI) POS17_3979
PSED: DM292_11235(benB) DM292_11240(benA)
AVN: Avin_08590(benA) Avin_08600(benB)
AVL: AvCA_08590(benA) AvCA_08600(benB)
AVD: AvCA6_08590(benA) AvCA6_08600(benB)
ACX: Achr_32830(benB) Achr_32840(benA)
PSYP: E5677_05460(benB) E5677_05465(benA)
ABM: ABSDF1497(benA)
ABY: ABAYE1704 ABAYE2555(benA) ABAYE2556(benB)
ABB: ABBFA_01573(antA_1) ABBFA_02333(cbdA) ABBFA_02334(cbdB_2)
ABZ: ABZJ_01350(benB) ABZJ_01351(benA) ABZJ_02153
ABAD: ABD1_12230(benB) ABD1_12240(benA) ABD1_18830
ACC: BDGL_000465(benB) BDGL_000466(benA)
ACI: ACIAD1436(benA) ACIAD1437(benB)
ASJ: AsACE_CH01474(benA) AsACE_CH01475(benB)
AID: CTZ23_07110(benB) CTZ23_07115(benA)
ADV: DJ533_10805(benA) DJ533_10810(benB)
AWU: BEN71_06060(benB) BEN71_06065(benA)
ACUM: C9E88_012195(benA) C9E88_012200(benB)
AGU: AS4_23670(benA) AS4_23680(benB)
ALW: FOB21_01925(benA) FOB21_01930(benB)
ADS: FPL17_10290(benA) FPL17_10295(benB)
MHC: MARHY0278 MARHY0279(benB)
MAD: HP15_1106
PSE: NH8B_1750(benA) NH8B_1751(benB)
RSC: RCFBP_11861(benB) RCFBP_11862(benA)
RSL: RPSI07_1886(benB) RPSI07_1887(benA)
RSY: RSUY_19260(cbdA) RSUY_19270(cbdB)
REH: H16_A1962(benB) H16_A1963(benA)
CNC: CNE_1c19320(benB) CNE_1c19330(benA)
CUH: BJN34_08560(benA) BJN34_08565(benB)
RME: Rmet_4882(benA) Rmet_4883(benB)
BMA: BMAA0186(benB) BMAA0187(benA)
BMV: BMASAVP1_1359(benB) BMASAVP1_1360(benA)
BML: BMA10229_1554(benB) BMA10229_1555(benA)
BMN: BMA10247_A0214(benB) BMA10247_A0215(benA)
BMAL: DM55_3531(benA) DM55_3532(benB)
BMAE: DM78_4625(benB) DM78_4626(benA)
BMAQ: DM76_4228(benA) DM76_4229(benB)
BMAF: DM51_3937(benB) DM51_3938(benA)
BMAZ: BM44_3493(benA) BM44_3494(benB)
BMAB: BM45_4329(benA) BM45_4330(benB)
BPS: BPSS1903(benA) BPSS1904(benB)
BPD: BURPS668_A2725(benA) BURPS668_A2726(benB)
BPSE: BDL_5309(benA) BDL_5310(benB)
BPSM: BBQ_4226(benB) BBQ_4227(benA)
BPSU: BBN_5368(benA) BBN_5369(benB)
BPSD: BBX_4474(benA) BBX_4475(benB)
BPZ: BP1026B_II2042(benB) BP1026B_II2043(benA)
BPK: BBK_4642(benA) BBK_4643(benB)
BPSH: DR55_5830(benB) DR55_5831(benA)
BPSA: BBU_4105(benB) BBU_4106(benA)
BPSO: X996_5628(benB) X996_5629(benA)
BUT: X994_5191(benB) X994_5192(benA)
BTQ: BTQ_3765(benB) BTQ_3766(benA)
BTJ: BTJ_4796(benB) BTJ_4797(benA)
BTZ: BTL_5582(benB) BTL_5583(benA)
BTD: BTI_5086(benB) BTI_5087(benA)
BTV: BTHA_4618(benA) BTHA_4619(benB)
BTHE: BTN_5253(benB) BTN_5254(benA)
BTHM: BTRA_5523(benB) BTRA_5524(benA)
BTHL: BG87_5598(benB) BG87_5599(benA)
BOK: DM82_6193(benA) DM82_6194(benB)
BOC: BG90_4470(benB) BG90_4471(benA)
BVE: AK36_5308 AK36_5309(benB)
BCJ: BCAS0495(benB) BCAS0496(benA)
BCEN: DM39_5091(cbdA) DM39_5092(benB)
BCEW: DM40_5134(benB) DM40_5135(cbdA)
BCEO: I35_7539(benB) I35_7540(benA)
BMK: DM80_4617(cbdA) DM80_4618(benB)
BMUL: NP80_3315(benB) NP80_3316
BCED: DM42_7059(benB) DM42_7060(cbdA)
BDL: AK34_5717(benB) AK34_5718(cbdA)
BUB: BW23_4427(benB) BW23_4428(benA)
BGO: BM43_5500(benB) BM43_5501(cbdA)
BUL: BW21_4621(benB) BW21_4622(benA)
BXE: Bxe_B0914(benB) Bxe_B0915(benA)
BXB: DR64_6235(benB) DR64_6236(benA)
PLG: NCTC10937_02994(antA_3)
CABA: SBC2_79720(cbdA) SBC2_79730(cbdB)
BPT: Bpet1398(benB) Bpet1399(benA)
AXX: ERS451415_00597(antA_1)
ACRA: BSY15_1218(benB) BSY15_1219
JAS: FJQ89_23190(benB) FJQ89_23195(benA)
HSE: Hsero_1308(cbeA) Hsero_1309(cbeB)
HRB: Hrubri_1093(cbeA2) Hrubri_1094(cbeB2) Hrubri_1134(cbeA) Hrubri_1135(cbeB)
MALI: EYF70_17210(benB) EYF70_17215(benA)
MUM: FCL38_01205(benA) FCL38_01210(benB)
AON: DEH84_15060(benA) DEH84_15065(benB)
AMIH: CO731_00668(cbdB) CO731_00669(cbdA)
SFD: USDA257_c54440(xylY) USDA257_c54450(cbdA)
RHK: Kim5_CH01087(benB) Kim5_CH01088(benA)
BOS: BSY19_2043(benB) BSY19_2044(benA)
MEE: DA075_16980(benB) DA075_16985(benA)
MTEA: DK419_08700(benA) DK419_08705(benB)
RUT: FIU92_22570(cbdB) FIU92_22575(cbdA)
JAN: Jann_1343
PZH: CX676_12375(benA) CX676_12380(benB)
PSF: PSE_2828(ben_A)
CID: P73_2151
LABT: FIU93_30120(cbdA)
ROT: FIV09_19515(cbdA) FIV09_19520(cbdB)
SPHR: BSY17_106(benB) BSY17_107
SMIC: SmB9_20300
RMUC: FOB66_02965(benA) FOB66_02970(benB)
AZL: AZL_a07340(benA) AZL_a07350(benB)
AZM: DM194_25870(benB) DM194_25875(benA)
MAV: MAV_5083
MIR: OCQ_16480
MTHN: 4412656_00399(antB)
MTER: 4434518_02877(cbdA) 4434518_02878(cbdB)
CGL: NCgl2320(Cgl2404) NCgl2321(Cgl2405)
CGB: cg2637(benA) cg2638(benB)
CGU: WA5_2320(BenA) WA5_2321(BenB)
CGM: cgp_2637(benA) cgp_2638
CMQ: B840_09740(benA) B840_09745(xylY)
NFA: NFA_28450
NFR: ERS450000_01244(antA)
RHA: RHA1_ro02384(benA) RHA1_ro02385(benB)
ROP: ROP_20980(benA) ROP_20990(benB)
RHB: NY08_2277
RRZ: CS378_14015(benA) CS378_14020(benB)
RHU: A3Q40_00992(cbdA) A3Q40_00993(cbdB)
RQI: C1M55_27395(benA) C1M55_27400(benB)
RRT: 4535765_04641(benA) 4535765_04642(benB)
RBY: CEJ39_00965(benA) CEJ39_00970(benB)
GPO: GPOL_c38890(benA) GPOL_c38900(benB)
GOC: CXX93_01335(benA)
GRU: GCWB2_11190(cbdB1) GCWB2_11195(cbdA2) GCWB2_20665(cbdA4) GCWB2_20670(cbdB3) GCWB2_22970(cbdA5)
GOM: D7316_01918(cbdA_1) D7316_01919(cbdB_1)
SALS: SLNWT_0066
ARN: CGK93_10495(benA) CGK93_10500(benB)
ARTP: E5206_02250(benA) E5206_02255(benB)
PSEY: GU243_07200(benA) GU243_07205(benB)
GLU: F0M17_16130(benB) F0M17_16135(benA)
KRS: EQG70_04355(benB) EQG70_04360(benA)
CIG: E7741_08345(benB) E7741_08350(benA)
RAIN: Rai3103_09960(benB)
NCA: Noca_0826
SEN: SACE_4378(benB) SACE_4379(benA)
SACE: GIY23_06600(benB) GIY23_06605(benA)
AMQ: AMETH_2178(benA) AMETH_2179(benB)
AMYC: CU254_24145(benB) CU254_24150(benA)
PDX: Psed_4287
PSEA: WY02_09515
 » show all
Reference
1  [PMID:214433]
  Authors
Yamaguchi M, Fujisawa H.
  Title
Characterization of NADH-cytochrome c reductase, a component of benzoate 1,2-dioxygenase system from Pseudomonas arvilla c-1.
  Journal
J Biol Chem 253:8848-53 (1978)
Reference
2  [PMID:7372624]
  Authors
Yamaguchi M, Fujisawa H.
  Title
Purification and characterization of an oxygenase component in benzoate 1,2-dioxygenase system from Pseudomonas arvilla C-1.
  Journal
J Biol Chem 255:5058-63 (1980)
Reference
3  [PMID:7130163]
  Authors
Yamaguchi M, Fujisawa H.
  Title
Subunit structure of oxygenase component in benzoate-1,2-dioxygenase system from Pseudomonas arvilla C-1.
  Journal
J Biol Chem 257:12497-502 (1982)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.10
IUBMB Enzyme Nomenclature: 1.14.12.10
ExPASy - ENZYME nomenclature database: 1.14.12.10
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.10
BRENDA, the Enzyme Database: 1.14.12.10
CAS: 9059-18-1

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