KEGG ENZYME: 1.14.12.11

ENZYME: 1.14.12.11

Entry

EC 1.14.12.11               Enzyme

Name

toluene dioxygenase;
toluene 2,3-dioxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Sysname

toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating)

Reaction(IUBMB)

toluene + NADH + H+ + O2 = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R03559]

Reaction(KEGG)

R03559;
(other) R09151 R09152

Substrate

toluene [CPD:C01455];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol [CPD:C04592];
NAD+ [CPD:C00003]

Comment

A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase, and a ferredoxin. Some other aromatic compounds, including ethylbenzene, 4-xylene and some halogenated toluenes, are converted into the corresponding cis-dihydrodiols.

History

EC 1.14.12.11 created 1992

Pathway

ec00623

Toluene degradation

ec00625

Chloroalkane and chloroalkene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology