KEGG   ENZYME: 1.14.12.12Help
Entry
EC 1.14.12.12               Enzyme                                 

Name
naphthalene 1,2-dioxygenase;
naphthalene dioxygenase;
naphthalene oxygenase;
NDO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
naphthalene,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Reaction(IUBMB)
naphthalene + NADH + H+ + O2 = (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+ [RN:R02968]
Reaction(KEGG)
Substrate
naphthalene [CPD:C00829];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(1R,2S)-1,2-dihydronaphthalene-1,2-diol [CPD:C04314];
NAD+ [CPD:C00003]
Comment
This enzyme is a member of the ring-hydroxylating dioxygenase (RHD) family of bacterial enzymes that play a critical role in the degradation of aromatic compounds, such as polycyclic aromatic hydrocarbons [5]. This enzyme comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.3, ferredoxin---NAD+ reductase), an iron-sulfur oxygenase, and ferredoxin. Requires Fe2+.
History
EC 1.14.12.12 created 1992
Pathway
ec00624  Polycyclic aromatic hydrocarbon degradation
ec00626  Naphthalene degradation
ec00642  Ethylbenzene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K14579  naphthalene 1,2-dioxygenase subunit alpha
K14580  naphthalene 1,2-dioxygenase subunit beta
Genes
PSC: A458_06720 A458_06725
PSTU: UIB01_22625 UIB01_22630
PBM: CL52_14610 CL52_14615
ALT: ambt_17890 ambt_17895
RMN: TK49_07955
BMK: DM80_1443(ndoB) DM80_1444(ndoC)
PNA: Pnap_2484 Pnap_2485
AJS: Ajs_3092 Ajs_3093
Taxonomy
Reference
1  [PMID:6874638]
  Authors
Ensley BD, Gibson DT.
  Title
Naphthalene dioxygenase: purification and properties of a terminal oxygenase component.
  Journal
J Bacteriol 155:505-11 (1983)
Reference
2  [PMID:234247]
  Authors
Jeffrey AM, Yeh HJ, Jerina DM, Patel TR, Davey JF, Gibson DT.
  Title
Initial reactions in the oxidation of naphthalene by Pseudomonas putida.
  Journal
Biochemistry 14:575-84 (1975)
Reference
3  [PMID:9634695]
  Authors
Kauppi B, Lee K, Carredano E, Parales RE, Gibson DT, Eklund H, Ramaswamy S
  Title
Structure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenase.
  Journal
Structure 6:571-86 (1998)
DOI:10.1016/S0969-2126(98)00059-8
Reference
4  [PMID:10692370]
  Authors
Parales RE, Lee K, Resnick SM, Jiang H, Lessner DJ, Gibson DT
  Title
Substrate specificity of naphthalene dioxygenase: effect of specific amino acids at the active site of the enzyme.
  Journal
J Bacteriol 182:1641-9 (2000)
DOI:10.1128/JB.182.6.1641-1649.2000
Reference
5  [PMID:17014090]
  Authors
Jouanneau Y, Meyer C, Jakoncic J, Stojanoff V, Gaillard J
  Title
Characterization of a naphthalene dioxygenase endowed with an exceptionally broad substrate specificity toward polycyclic aromatic hydrocarbons.
  Journal
Biochemistry 45:12380-91 (2006)
DOI:10.1021/bi0611311
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.12
IUBMB Enzyme Nomenclature: 1.14.12.12
ExPASy - ENZYME nomenclature database: 1.14.12.12
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.12
BRENDA, the Enzyme Database: 1.14.12.12
CAS: 9074-04-8

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