Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
2-halobenzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating, dehalogenating, decarboxylating)
a 2-halobenzoate + NADH + H+ + O2 = catechol + a halide anion + NAD+ + CO2 [RN:
A multicomponent enzyme system composed of a dioxygenase component and an electron transfer component. The latter contains FAD. The enzyme, characterized from the bacterium Burkholderia cepacia 2CBS, has a broad substrate specificity. Substrates include 2-fluorobenzoate, 2-chlorobenzoate, 2-bromobenzoate, and 2-iodobenzoate, which are processed in this order of preference.
EC 22.214.171.124 created 1992, modified 2012
ec01120 Microbial metabolism in diverse environments
K08686 2-halobenzoate 1,2-dioxygenase large subunit
K20808 2-halobenzoate 1,2-dioxygenase small subunit
Fetzner S, Muller R, Lingens F.
Degradation of 2-chlorobenzoate by Pseudomonas cepacia 2CBS.
Fetzner S, Muller R, Lingens F
Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from Pseudomonas cepacia 2CBS.
Haak B, Fetzner S, Lingens F
Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS.
UM-BBD (Biocatalysis/Biodegradation Database): 126.96.36.199