cnbA (gene name)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
nitrobenzene,NADH:oxygen oxidoreductase (1,2-hydroxylating, nitrite-releasing)
nitrobenzene + NADH + O2 = catechol + nitrite + NAD+ [RN:
This enzyme is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC
, ferredoxin---NAD+ reductase), and an alpha3beta3 oxygenase. The enzyme forms of a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It can typically act on many different nitroaromatic compounds, including chlorinated species. Enzymes found in different strains may have different substrate preferences. Requires Fe2+.
EC 188.8.131.52 created 2015
ec01120 Microbial metabolism in diverse environments
K14579 naphthalene 1,2-dioxygenase subunit alpha
K14580 naphthalene 1,2-dioxygenase subunit beta
PPI: ND055(nahAc) ND056(nahAd)
PFK: PFAS1_28610 PFAS1_28615
PSC: A458_06720 A458_06725
PSTU: UIB01_22625 UIB01_22630
PBM: CL52_14610 CL52_14615
ALT: ambt_17890 ambt_17895
BMK: DM80_1443(ndoB) DM80_1444(ndoC)
PARB: CJU94_38655 CJU94_38660 » show all
Parales JV, Parales RE, Resnick SM, Gibson DT
Enzyme specificity of 2-nitrotoluene 2,3-dioxygenase from Pseudomonas sp. strain JS42 is determined by the C-terminal region of the alpha subunit of the oxygenase component.
Lessner DJ, Johnson GR, Parales RE, Spain JC, Gibson DT
Molecular characterization and substrate specificity of nitrobenzene dioxygenase from Comamonas sp. strain JS765.
Liu H, Wang SJ, Zhang JJ, Dai H, Tang H, Zhou NY
Patchwork assembly of nag-like nitroarene dioxygenase genes and the 3-chlorocatechol degradation cluster for evolution of the 2-chloronitrobenzene catabolism pathway in Pseudomonas stutzeri ZWLR2-1.
Singh D, Kumari A, Ramaswamy S, Ramanathan G
Expression, purification and substrate specificities of 3-nitrotoluene dioxygenase from Diaphorobacter sp. strain DS2.