KEGG   ENZYME: 1.14.12.24Help
Entry
EC 1.14.12.24               Enzyme                                 

Name
2,4-dinitrotoluene dioxygenase;
dntA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
2,4-dinitrotoluene,NADH:oxygen oxidoreductase (4,5-hydroxylating, nitrite-releasing)
Reaction(IUBMB)
2,4-dinitrotoluene + NADH + O2 = 4-methyl-5-nitrocatechol + nitrite + NAD+ [RN:R09233]
Reaction(KEGG)
Substrate
2,4-dinitrotoluene [CPD:C11006];
NADH [CPD:C00004];
O2 [CPD:C00007]
Product
4-methyl-5-nitrocatechol [CPD:C18315];
nitrite [CPD:C00088];
NAD+ [CPD:C00003]
Comment
This enzyme, characterized from the bacterium Burkholderia sp. strain DNT, is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin---NAD+ reductase), and an alpha3beta3 oxygenase. The enzyme forms a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It does not act on nitrobenzene. cf. EC 1.14.12.23, nitroarene dioxygenase.
History
EC 1.14.12.24 created 2015
Pathway
ec00633  Nitrotoluene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K14579  naphthalene 1,2-dioxygenase subunit alpha
K14580  naphthalene 1,2-dioxygenase subunit beta
Genes
PPI: ND055(nahAc) ND056(nahAd)
PSC: A458_06720 A458_06725
PSTU: UIB01_22625 UIB01_22630
PBM: CL52_14610 CL52_14615
PFK: PFAS1_28610 PFAS1_28615
ALT: ambt_17890 ambt_17895
RMN: TK49_07955
BMK: DM80_1443(ndoB) DM80_1444(ndoC)
PARB: CJU94_38660
PNA: Pnap_2484 Pnap_2485
 » show all
Taxonomy
Reference
1  [PMID:8759857]
  Authors
Suen WC, Haigler BE, Spain JC
  Title
2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase.
  Journal
J Bacteriol 178:4926-34 (1996)
DOI:10.1128/JB.178.16.4926-4934.1996
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.24
IUBMB Enzyme Nomenclature: 1.14.12.24
ExPASy - ENZYME nomenclature database: 1.14.12.24
BRENDA, the Enzyme Database: 1.14.12.24

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