KEGG   ENZYME: 1.14.12.25Help
Entry
EC 1.14.12.25               Enzyme                                 

Name
p-cumate 2,3-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
4-isopropylbenzoate:oxygen 2,3-oxidoreductase
Reaction(IUBMB)
p-cumate + NADH + H+ + O2 = (2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate + NAD+ [RN:R05247]
Reaction(KEGG)
Substrate
p-cumate [CPD:C06578];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate [CPD:C06579];
NAD+ [CPD:C00003]
Comment
The enzyme, characterized from several Pseudomonas strains, is involved in the degradation of p-cymene and p-cumate. It comprises four components: a ferredoxin, a ferredoxin reductase, and two subunits of a catalytic component. The enzyme can also act on indole, transforming it to the water-insoluble blue dye indigo.
History
EC 1.14.12.25 created 2016
Pathway
ec00622  Xylene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K10619  p-cumate 2,3-dioxygenase subunit alpha
K16303  p-cumate 2,3-dioxygenase subunit beta
Genes
PAEV: N297_2148
PAEI: N296_2148
PAF: PAM18_2960
PNC: NCGM2_3063
PAEB: NCGM1900_4398
PDK: PADK2_15235
PSG: G655_14685
PRP: M062_10830
PAEP: PA1S_15265
PAEM: U769_14865
PAEL: T223_16545
PAEG: AI22_18505
PAEC: M802_2145
PAEO: M801_2147
PPX: T1E_4248(cbdC) T1E_4249
MHAS: MHAS_04025(bnzB) MHAS_04026(cbdA_2)
ROP: ROP_46330(cmtA2) ROP_46340(cmtA1)
RRT: 4535765_00187(cmtA1_1) 4535765_00188(cmtA2_1) 4535765_00374(cmtA2_2) 4535765_00375(cmtA1_2)
PDX: Psed_4289
 » show all
Taxonomy
Reference
1  [PMID:845117]
  Authors
DeFrank JJ, Ribbons DW
  Title
p-cymene pathway in Pseudomonas putida: initial reactions.
  Journal
J Bacteriol 129:1356-64 (1977)
Reference
2  [PMID:7204334]
  Authors
Wigmore GJ, Ribbons DW
  Title
p-Cymene pathway in Pseudomonas putida: selective enrichment of defective mutants by using halogenated substrate analogs.
  Journal
J Bacteriol 143:816-24 (1980)
Reference
3  [PMID:7592495]
  Authors
Eaton RW, Chapman PJ
  Title
Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids.
  Journal
J Bacteriol 177:6983-8 (1995)
DOI:10.1128/JB.177.23.6983-6988.1995
Reference
4  [PMID:8631713]
  Authors
Eaton RW.
  Title
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.
  Journal
J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.25
IUBMB Enzyme Nomenclature: 1.14.12.25
ExPASy - ENZYME nomenclature database: 1.14.12.25
BRENDA, the Enzyme Database: 1.14.12.25

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