KEGG   ENZYME: 1.14.12.26Help
Entry
EC 1.14.12.26               Enzyme                                 

Name
chlorobenzene dioxygenase;
TecA
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
chlorobenzene,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Reaction(IUBMB)
chlorobenzene + NADH + H+ + O2 = (1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R06856]
Reaction(KEGG)
R06856;
(other) R05429 R06831
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Substrate
chlorobenzene [CPD:C06990];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol [CPD:C12837];
NAD+ [CPD:C00003]
Comment
This bacterial enzyme is a class IIB dioxygenase, comprising three components - a heterodimeric terminal dioxygenase, a ferredoxin protein, and a ferredoxin reductase. The enzyme acts on a range of aromatic compounds, including mono-, di-, tri-, and tetra-chlorinated benzenes and toluenes.
History
EC 1.14.12.26 created 2018
Pathway
ec00361  Chlorocyclohexane and chlorobenzene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K03268  benzene/toluene/chlorobenzene dioxygenase subunit alpha
K16268  benzene/toluene/chlorobenzene dioxygenase subunit beta
Genes
PPF: Pput_2880 Pput_2881
PPX: T1E_4266(todC1) T1E_4267(todC2)
PMON: X969_10930 X969_10935
PMOT: X970_10585 X970_10590
BPT: Bpet3738 Bpet3739
Taxonomy
Reference
1  [PMID:8526500]
  Authors
Spiess E, Sommer C, Gorisch H
  Title
Degradation of 1,4-dichlorobenzene by Xanthobacter flavus 14p1.
  Journal
Appl Environ Microbiol 61:3884-8 (1995)
Reference
2  [PMID:9148781]
  Authors
Sommer C, Gorisch H
  Title
Enzymology of the degradation of (di)chlorobenzenes by Xanthobacter flavus 14p1.
  Journal
Arch Microbiol 167:384-91 (1997)
DOI:10.1007/s002030050459
Reference
3  [PMID:9249026]
  Authors
Beil S, Happe B, Timmis KN, Pieper DH
  Title
Genetic and biochemical characterization of the broad spectrum chlorobenzene dioxygenase from Burkholderia sp. strain PS12--dechlorination of 1,2,4,5-tetrachlorobenzene.
  Journal
Eur J Biochem 247:190-9 (1997)
Reference
4  [PMID:9791099]
  Authors
Beil S, Mason JR, Timmis KN, Pieper DH
  Title
Identification of chlorobenzene dioxygenase sequence elements involved in dechlorination of 1,2,4,5-tetrachlorobenzene.
  Journal
J Bacteriol 180:5520-8 (1998)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.26
IUBMB Enzyme Nomenclature: 1.14.12.26
ExPASy - ENZYME nomenclature database: 1.14.12.26
BRENDA, the Enzyme Database: 1.14.12.26

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