KEGG ENZYME: 1.14.12.3

ENZYME: 1.14.12.3

Entry

EC 1.14.12.3 Enzyme

Name

benzene 1,2-dioxygenase;
benzene hydroxylase;
benzene dioxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Sysname

benzene,NADH:oxygen oxidoreductase (1,2-hydroxylating)

Reaction(IUBMB)

benzene + NADH + H+ + O2 = cis-cyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R03543]

Reaction(KEGG)

R03543

Substrate

benzene [CPD:C01407];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

cis-cyclohexa-3,5-diene-1,2-diol [CPD:C04091];
NAD+ [CPD:C00003]

Comment

A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase and ferredoxin. Requires Fe2+.

History

EC 1.14.12.3 created 1972

Pathway

ec00361

Chlorocyclohexane and chlorobenzene degradation

ec00362

Benzoate degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K03268

benzene/toluene/chlorobenzene dioxygenase subunit alpha

K16268

benzene/toluene/chlorobenzene dioxygenase subunit beta

Genes

PPF:	Pput_2880 Pput_2881

PPX:	T1E_4266(todC1) T1E_4267(todC2)

PMON:

X969_10930 X969_10935

PMOT:

X970_10585 X970_10590

BPT:	Bpet3738 Bpet3739

Reference

1 [PMID:4298226]

Authors

Gibson DT, Koch JR, Kallio RE.

Title

Oxidative degradation of aromatic hydrocarbons by microorganisms. I. Enzymatic formation of catechol from benzene.

Journal

Biochemistry 7:2653-62 (1968)
DOI:10.1021/bi00847a031

Other DBs

ExplorEnz - The Enzyme Database:

1.14.12.3

IUBMB Enzyme Nomenclature:

1.14.12.3

ExPASy - ENZYME nomenclature database:

1.14.12.3

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.12.3

BRENDA, the Enzyme Database:

1.14.12.3

CAS:

9075-66-5

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