KEGG   ENZYME: 1.14.13.102Help
Entry
EC 1.14.13.102              Enzyme                                 

Name
psoralen synthase;
CYP71AJ1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(+)-marmesin,NADPH:oxygen oxidoreductase
Reaction(IUBMB)
(+)-marmesin + NADPH + H+ + O2 = psoralen + NADP+ + acetone + 2 H2O [RN:R08207]
Reaction(KEGG)
Substrate
(+)-marmesin [CPD:C09276];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
psoralen [CPD:C09305];
NADP+ [CPD:C00006];
acetone [CPD:C00207];
H2O [CPD:C00001]
Comment
This microsomal cytochrome P-450-dependent enzyme is specific for (+)-marmesin, and to a much lesser extent 5-hydroxymarmesin, as substrate. Furanocoumarins protect plants from fungal invasion and herbivore attack. (+)-Columbianetin, the angular furanocoumarin analogue of the linear furanocoumarin (+)-marmesin, is not a substrate for the enzyme but it is a competitive inhibitor.
History
EC 1.14.13.102 created 2007
Orthology
K21714  psoralen synthase
Reference
1  [PMID:17068340]
  Authors
Larbat R, Kellner S, Specker S, Hehn A, Gontier E, Hans J, Bourgaud F, Matern U.
  Title
Molecular cloning and functional characterization of psoralen synthase, the first committed monooxygenase of furanocoumarin biosynthesis.
  Journal
J Biol Chem 282:542-54 (2007)
DOI:10.1074/jbc.M604762200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.102
IUBMB Enzyme Nomenclature: 1.14.13.102
ExPASy - ENZYME nomenclature database: 1.14.13.102
BRENDA, the Enzyme Database: 1.14.13.102

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