KEGG ENZYME: 1.14.13.107

ENZYME: 1.14.13.107

Entry

EC 1.14.13.107              Enzyme

Name

limonene 1,2-monooxygenase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

limonene,NAD(P)H:oxygen oxidoreductase

Reaction(IUBMB)

(1) (S)-limonene + NAD(P)H + H+ + O2 = 1,2-epoxymenth-8-ene + NAD(P)+ + H2O [RN:R09385 R09389];
(2) (R)-limonene + NAD(P)H + H+ + O2 = 1,2-epoxymenth-8-ene + NAD(P)+ + H2O [RN:R06398 R09393]

Reaction(KEGG)

R06398 R09385 R09389 R09393

Substrate

(S)-limonene [CPD:C00521];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007];
(R)-limonene [CPD:C06099]

Product

1,2-epoxymenth-8-ene;
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Comment

A flavoprotein (FAD). Limonene is the most widespread terpene and is formed by more than 300 plants. Rhodococcus erythropolis DCL14, a Gram-positive bacterium, is able to grow on both (S)-limonene and (R)-limonene as the sole source of carbon and energy. NADPH can act instead of NADH, although more slowly. It has not been established if the product formed is optically pure or a mixture of two enantiomers.

History

EC 1.14.13.107 created 2009

Pathway

ec00903

Limonene degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K14733

limonene 1,2-monooxygenase

Genes

MES:

Meso_3721

PLA:

Plav_1646

PMOB:

HG718_00680

PECT:

BN1012_Phect1006

RBS:	RHODOSMS8_02624(limB)

BJA:	bll0895(bll0895)

BJU:	BJ6T_09070

BJP:	RN69_04430

BRA:

BRADO0508

BBT:

BBta_7675

BRS:

S23_69110

AOL:

S58_71450

BRC:	BCCGELA001_35180

BRAD:

BF49_7289

BIC:	LMTR13_01245

BRO:	BRAD285_7739

BRK:	CWS35_04745

BOT:	CIT37_09865

BRQ:	CIT40_31045

BGQ:	X265_35500

BGZ:	XH91_33915

BSYM:

CIT39_30085

BBET:

F8237_13860

BARH:

WN72_47140

BVZ:	BRAD3257_8837(limB)

BEL:	BE61_10590(limB) BE61_31350

BDG:	LPJ38_03025

BSEP:

HAP48_0017150

BQB:	J4P68_0020695

BSEI:

KMZ68_25605

BBAN:

J4G43_051640

BCAN:

BcanWSM471_36345

BAUT:

QA635_41240

BBRA:

QA636_42310

BGK:	IC762_02665

BCOU:

IC761_35520

BPAH:

QA639_41415

BXN:	I3J27_38540

BROS:

QUH67_34165

BRAZ:

LRP30_43665

BOY:	LQG66_24000

BRAY:

ML401_33610

RBK:	E0H22_24760

RHOR:

ONR75_02725

TRB:	HB776_22705

TALZ:

RPMA_27520

APRA:

G3A50_17825

CMET:

K6K41_27575

RBM:

TEF_06950

CMB:	CSW64_14700

PZU:

PHZ_c1133

PHB:	HYN04_12230

CAUL:

KCG34_21615

TSV:	DSM104635_00473(limB_1)

RDE:

RD1_3871

RLI:	RLO149_c005640(limB)

RFU:	ROLI_011030(limB)

THAA:

CFI11_01855

YRH:	AABB31_09690

SPFD:

SPYCA_3648

SPHD:

HY78_18285 HY78_18375 HY78_18440

SPHM:

G432_04655

SPKC:

KC8_00760 KC8_08220

SPHA:

D3Y57_19625

SBIN:

SBA_ch1_21710

SRAD:

LLW23_09220 LLW23_09405

SSY:

SLG_34780

SPHB:

EP837_02941

SAMI:

SAMIE_1019300 SAMIE_1033590

SPAG:

sphantq_03283(limB_1)

SPHJ:

BSL82_10485 BSL82_10530

NTD:	EGO55_02800 EGO55_04330 EGO55_04790

CAEN:

K5X80_01000 K5X80_05470 K5X80_07285

RFL:

Rmf_17230

SRF:	LHU95_05900

SHUM:

STHU_16220

SVC:	STVA_13500

STEL:

STAQ_12270

MTAR:

DF168_00705(limB)

MTU:

Rv3618

MTV:

RVBD_3618

MTC:

MT3720

MRA:

MRA_3654

MTF:	TBFG_13649

MTB:	TBMG_03655

MTK:	TBSG_03680

MTZ:	TBXG_003627

MTG:	MRGA327_22290

MTI:	MRGA423_22835

MTE:	CCDC5079_3356

MTUR:

CFBS_3834

MTL:	CCDC5180_3308

MTO:	MTCTRI2_3683

MTD:

UDA_3618

MTN:	ERDMAN_3962

MTJ:	J112_19455

MTUB:

MT7199_3681

MTUC:

J113_25305

MTUE:

J114_19330

MTX:	M943_18580

MTUL:

TBHG_03556

MTUT:

HKBT1_3819

MTUU:

HKBT2_3827

MTQ:	HKBS1_3831

MCE:	MCAN_36301

MCQ:	BN44_120007

MCV:	BN43_90117

MCX:	BN42_90118

MCZ:	BN45_100130

MPA:	MAP_0445c MAP_1645

MAO:	MAP4_2193 MAP4_3422

MAVI:

RC58_10915 RC58_16975

MAVU:

RE97_10925 RE97_17010

MAV:	MAV_0538 MAV_2779

MIT:	OCO_04520 OCO_26110

MIA:	OCU_04550 OCU_25970

MID:	MIP_00873 MIP_03599 MIP_03607 MIP_03648

MYO:	OEM_04580 OEM_24600

MCHI:

AN480_02260 AN480_13590 AN480_13625 AN480_13760

MIR:	OCQ_04680 OCQ_24690

MMAL:

CKJ54_02180 CKJ54_11660 CKJ54_11695 CKJ54_11805

MLP:

MLM_2061

MMAN:

MMAN_12910 MMAN_47420

MSER:

MTY59_23550 MTY59_41620 MTY59_41690 MTY59_41930

MSA:	Mycsm_02075 Mycsm_05927

MUL:	MUL_2949 MUL_4194

MMI:	MMAR_2824 MMAR_5115

MMAE:

MMARE11_27450 MMARE11_49370

MLI:	MULP_02587 MULP_05376

MPSE:

MPSD_03650 MPSD_29110

MSHO:

MSHO_06160 MSHO_42350

MMC:

Mmcs_4772

MKM:

Mkms_4858

MJL:

Mjls_5158

MMM:	W7S_02200 W7S_12680

MKN:	MKAN_00970 MKAN_12460

MYV:	G155_01850 G155_26930

MYE:	AB431_26805

MHAD:

B586_04220

MSHG:

MSG_02711 MSG_04611

MFJ:	MFLOJ_05070 MFLOJ_40140

MSTO:

MSTO_06410 MSTO_44100

MSIM:

MSIM_28550 MSIM_43700 MSIM_50410

MSAK:

MSAS_08110 MSAS_29750

MKU:	I2456_13905 I2456_25950

MLW:	MJO58_14290 MJO58_25370

MXE:	MYXE_04650 MYXE_24370

MNV:	MNVI_12960 MNVI_42130

MPAG:

C0J29_16250 C0J29_28115

MNM:	MNVM_22970

MGOR:

H0P51_15250 H0P51_26100

MCOO:

MCOO_10060 MCOO_27810

MBAI:

MB901379_02609(limB_2) MB901379_04514(limB_7)

MSEO:

MSEO_15320 MSEO_35140

MHEK:

JMUB5695_00423 JMUB5695_02356

MGAU:

MGALJ_02000

MLJ:	MLAC_46780

MBRD:

MBRA_19490 MBRA_38220 MBRA_57940

MSHJ:

MSHI_38060

MMAM:

K3U93_13005 K3U93_22955

MSPG:

F6B93_11665 F6B93_20790

MOT:	LTS72_10090 LTS72_27590

MPAA:

MKK62_02170 MKK62_12710

MMEH:

M5I08_21760

MKY:	IWGMT90018_29100 IWGMT90018_58090

MADI:

A7U43_06315

MWU:	PT015_06215 PT015_15805

MYW:	BVC93_09640

MSM:	MSMEG_6107

MSG:	MSMEI_5948

MSB:	LJ00_30200

MSN:	LI99_30205

MSH:	LI98_30210

MVA:

Mvan_1925

MGI:

Mflv_4429

MSP:	Mspyr1_38290

MCB:	Mycch_1625

MNE:	D174_25035

MYN:	MyAD_24570

MFT:	XA26_26540 XA26_54530

MPHL:

MPHLCCUG_00069(limB_1)

MVQ:

MYVA_1767

MRH:	MycrhN_6318

MTHN:

4412656_02907(limB_3) 4412656_04146(limB_7)

MDU:	MDUV_25640

MAUU:

NCTC10437_01764(limB_3)

MMOR:

MMOR_47660

MAIC:

MAIC_04690

MALV:

MALV_17840 MALV_41540

MTY:	MTOK_21380

MPSC:

MPSYJ_46970

MARZ:

MARA_22400

MGAD:

MGAD_21990

MHEV:

MHEL_27150

MSAR:

MSAR_01880

MANY:

MANY_33580

MAUB:

MAUB_04320 MAUB_27850

MPOF:

MPOR_22530

MPHU:

MPHO_08430 MPHO_42500

MMUC:

C1S78_013955 C1S78_025995

MMAT:

MMAGJ_33030 MMAGJ_57470

MBOK:

MBOE_16540 MBOE_48350

MFG:	K6L26_04875 K6L26_19320

MSEI:

MSEDJ_05290

MFLV:

NCTC10271_00068(limB_1)

MCEE:

MCEL_32950

MMON:

EWR22_25790

MGO:	AFA91_09645 AFA91_10240

MHOL:

K3U96_02815 K3U96_26415

MSEN:

K3U95_16900 K3U95_26025

MPAE:

K0O64_26315

MRF:	MJO55_08735

MDF:	K0O62_26295

MCRO:

MI149_26615

MAUS:

JN090_08575

MFRE:

EXE63_18365

MAB:

MAB_0527c

MMV:	MYCMA_0262

MABB:

MASS_0495

MABL:

MMASJCM_0514

MCHE:

BB28_02480

MIZ:	BAB75_03225

MSTE:

MSTE_00475

MSAO:

MYCSP_02220

MSAL:

DSM43276_00436(limB_1)

MJD:	JDM601_3901

MTER:

4434518_03775(limB_5)

MMIN:

MMIN_12950

MHIB:

MHIB_31620

MHER:

K3U94_21270

MVM:	MJO54_21165

NCY:	NOCYR_3944(limB)

NSR:	NS506_03939(limB)

NOD:	FOH10_22590

NTC:	KHQ06_19600

NHU:	H0264_34215

NYA:	LTV02_38705

NYU:	D7D52_20465

RHA:	RHA1_ro09085 RHA1_ro11046(limB)

RER:

RER_46450

REY:

O5Y_21925

REB:	XU06_22040

RQI:	C1M55_23100 C1M55_23375

ROP:	ROP_46710(limB)

RHOD:

AOT96_03140 AOT96_03410

RKO:	JWS14_00135 JWS14_15610 JWS14_21745

RGO:	KYT97_16335 KYT97_19820 KYT97_29955

ROZ:	CBI38_33465

RPSK:

JWS13_23210 JWS13_31105 JWS13_31250

RANT:

RHODO2019_03425

RHOO:

RA302_22690

RHOJ:

JVH1_16620

RHOH:

CPI83_07045

REQ:

REQ_44050

PDEF:

P9209_05455 P9209_25285

PSOW:

ABI214_15190 ABI214_15635

GPO:	GPOL_c09000

GTA:	BCM27_13075 BCM27_17640 BCM27_22070

GRU:	GCWB2_12650(limB1)

GAV:	C5O27_15505

GOD:	GKZ92_11400

GOS:	QAD21_21030

GOJ:	GR168_20085

GAM:	GII34_20715

GJI:	H1R19_12205

GHN:	MVF96_21040

SCO:	SCP1.240c(mmyO)

SGRF:

SGFS_035490 SGFS_079850

SHY:

SHJG_4007

SHO:	SHJGH_3772

SRC:	M271_44285

SBH:

SBI_00943

SALS:

SLNWT_0923

SALU:

DC74_7652

SLX:	SLAV_32745(limB7)

SFK:

KY5_6779

SKA:	CP970_06780

STSU:

B7R87_29655

SXN:	IAG42_23100

SLON:

LGI35_34410

SKG:	KJK29_13150 KJK29_13510

SCT:	SCAT_0119(limB)

SCY:	SCATT_01270

KIT:	CFP65_0771

STRI:

C7M71_007320

SUBT:

KPL76_03175

NANO:

G5V58_08720

NAL:	B005_2518(limB)

SPIQ:

OHA34_04290

NFS:	OIE67_47310

GOB:

Gobs_2267

BSD:	BLASA_2848(limB) BLASA_3512(limB2)

MMAR:

MODMU_2453(limB)

SACG:

FDZ84_00685

AMYB:

BKN51_26440

AMYY:

YIM_29395(limB8)

AACD:

LWP59_06125

AROO:

NQK81_32835

ARHD:

VSH64_08020 VSH64_10315

ACYN:

ORV05_20875

ACOR:

LCL61_35475

AMYZ:

HUW46_00169(limB)

ACAB:

QRX50_38320

ANW:	QP939_15025

PDX:

Psed_5126

PSEA:

WY02_18195

PSEE:

FRP1_17430 FRP1_24600

PSEH:

XF36_23280

PSEQ:

AD006_03785 AD006_24885 AD006_28245 AD006_28495 AD006_32110

PECQ:

AD017_04475 AD017_11605 AD017_28640 AD017_28885

PAUT:

Pdca_46170

PBRO:

HOP40_17895

PPEL:

H6H00_08485 H6H00_21510 H6H00_29285 H6H00_30390

PSDS:

K1T35_36810 K1T35_42075

SESP:

BN6_21310(limB)

SSYI:

EKG83_15850

AHM:	TL08_15815

PMAD:

BAY61_15565

ACTY:

OG774_09055

VER:	HUT12_14515

AIH:	Aiant_03690

ACTR:

Asp14428_19790

ACTL:

L3i22_052630

AOU:	ACTOB_005289

DVC:	Dvina_12855 Dvina_24745 Dvina_26620

DAUR:

Daura_27010

DMAT:

Dmats_12415 Dmats_20355 Dmats_22065

AEY:	CDG81_10655 CDG81_21480

EKE:	EK0264_06915

ABAI:

IMCC26256_11725

SBAE:

DSM104329_00232(limB_1) DSM104329_00236(limB_2)

AYM:	YM304_25770(limB)

IAM:	HC251_22820 HC251_24280

DHI:	LH044_02130

TBH:	Tbon_00310

TFLA:

O0235_08360 O0235_12640

» show all

Reference

1 [PMID:10224006]

Authors

van der Werf MJ, Swarts HJ, de Bont JA

Title

Rhodococcus erythropolis DCL14 contains a novel degradation pathway for limonene.

Journal

Appl Environ Microbiol 65:2092-102 (1999)
DOI:10.1128/AEM.65.5.2092-2102.1999

Sequence

[ag:CAC20855]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.13.107

IUBMB Enzyme Nomenclature:

1.14.13.107

ExPASy - ENZYME nomenclature database:

1.14.13.107

UM-BBD (Biocatalysis/Biodegradation Database):

1.14.13.107

BRENDA, the Enzyme Database:

1.14.13.107

DBGET integrated database retrieval system