KEGG   ENZYME: 1.14.13.111Help
Entry
EC 1.14.13.111              Enzyme                                 

Name
methanesulfonate monooxygenase (NADH);
mesylate monooxygenase;
mesylate,reduced-FMN:oxygen oxidoreductase;
MsmABC;
methanesulfonic acid monooxygenase;
MSA monooxygenase;
MSAMO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
methanesulfonate,NADH:oxygen oxidoreductase
Reaction(IUBMB)
methanesulfonate + NADH + H+ + O2 = formaldehyde + NAD+ + sulfite + H2O [RN:R09513]
Reaction(KEGG)
Substrate
methanesulfonate [CPD:C11145];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
formaldehyde [CPD:C00067];
NAD+ [CPD:C00003];
sulfite [CPD:C00094];
H2O [CPD:C00001]
Comment
A flavoprotein. Methanesulfonate is the simplest of the sulfonates and is a substrate for the growth of certain methylotrophic microorganisms. Compared with EC 1.14.14.5, alkanesulfonate monooxygenase, this enzyme has a restricted substrate range that includes only the short-chain aliphatic sulfonates (methanesulfonate to butanesulfonate) and excludes all larger molecules, such as arylsulfonates [1]. The enzyme from the bacterium Methylosulfonomonas methylovora is a multicomponent system comprising a hydroxylase, a reductase (MsmD) and a ferredoxin (MsmC). The hydroxylase has both large (MsmA) and small (MsmB) subunits, with each large subunit containing a Rieske-type [2Fe-2S] cluster. cf. EC 1.14.14.34, methanesulfonate monooxygenase (FMNH2).
History
EC 1.14.13.111 created 2009 as EC 1.14.14.6, transferred 2010 to EC 1.14.13.111, modified 2016
Pathway
ec00920  Sulfur metabolism
Orthology
K16968  methanesulfonate monooxygenase subunit alpha
K16969  methanesulfonate monooxygenase subunit beta
Genes
BCT: GEM_5811 GEM_5812
BUE: BRPE67_FCDS01800
PPK: U875_05270 U875_05275
PPNO: DA70_22340 DA70_22345
PRB: X636_04320 X636_04325
BPA: BPP0512 BPP0513
BBR: BB0517 BB0518
BBM: BN115_0496 BN115_0497
BBH: BN112_2903 BN112_2904
BBX: BBS798_0510 BBS798_0511
MPT: Mpe_A0919(msmS2) Mpe_A0920(msmB)
FIL: BN1229_v1_3548(msmB) BN1229_v1_3549(msmA)
FIY: BN1229_v1_2374(msmA) BN1229_v1_2375(msmB)
 » show all
Taxonomy
Reference
1  [PMID:10094704]
  Authors
de Marco P, Moradas-Ferreira P, Higgins TP, McDonald I, Kenna EM, Murrell JC
  Title
Molecular analysis of a novel methanesulfonic acid monooxygenase from the methylotroph Methylosulfonomonas methylovora.
  Journal
J Bacteriol 181:2244-51 (1999)
  Sequence
Reference
2  [PMID:8932698]
  Authors
Higgins TP, Davey M, Trickett J, Kelly DP, Murrell JC
  Title
Metabolism of methanesulfonic acid involves a multicomponent monooxygenase enzyme.
  Journal
Microbiology 142 ( Pt 2):251-60 (1996)
DOI:10.1099/13500872-142-2-251
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.111
IUBMB Enzyme Nomenclature: 1.14.13.111
ExPASy - ENZYME nomenclature database: 1.14.13.111
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.111
BRENDA, the Enzyme Database: 1.14.13.111

DBGET integrated database retrieval system